作者: Staples, J. Erin ; Panella, Amanda J. ; Laven, Janeen ; Gibney, Katherine B. ; Sejvar, James J. ; Basile, Alison J. ; Fischer, Marc ; Prince, Harry E.

ABSTRACT.West Nile virus (WNV) IgM antibodies typically indicate a recent infection. However, WNV IgM antibodies can remain detectable for months to years following illness onset. We found that 23% (11/47) of samples tested with a WNV ELISA and 43% (20/47) of samples tested with WNV microsphere immunoassay (MIA) at 16–19 months following WNV illness onset were positive for IgM antibodies. The proportion of samples testing positive for WNV IgM by ELISA decreased over time, but 5% (2/44) of individuals remained positive at 60–63 months after their acute illness and 4% (2/50) were WNV IgM equivocal at 72–81 months. Testing by MIA showed the same general trend of decreased proportion positive over time though the rates of positivity were higher at most time points compared with the ELISA, including 6% (3/50) of participant’s samples identified as IgM positive by MIA at 72–81 months post their acute illness. With the MIA, there also was a high proportion of samples with nonspecific results at each time point; average of 23% across all time points. Clinicians and public health officials should consider these findings along with clinical and epidemiologic data when interpreting WNV IgM antibody test results.

2021-09-20·Journal of Clinical Microbiology2区 · 医学

Trichomonas vaginalis Detection in Urogenital Specimens from Symptomatic and Asymptomatic Men and Women by Use of the cobas TV/MG Test

2区 · 医学

Article

作者: Eisenberg, David ; McGowin, Chris L. ; Ermel, Aaron ; Marlowe, Elizabeth M. ; Kaplan, Clair ; Chavoustie, Steven ; Krishnamurthy, Smitha ; Bertuzis, Rasa ; Nye, Melinda B. ; Rao, Arundhati ; Chan, Philip A. ; Mena, Leandro ; Pacheco, Sixto ; Xiao, Li ; Mohan, Ruchika ; Waites, Ken B. ; Van Der Pol, Barbara ; Arcenas, Rodney ; Taylor, Stephanie N.

2021-05-01·Diagnostic Microbiology and Infectious Disease4区 · 医学

Relationship between DiaSorin Liaison Treponema pallidum antibody indices and confirmatory assay results in the reverse syphilis testing algorithm

4区 · 医学

Article

作者: Schwab, Dale A ; Prince, Harry E ; Marlowe, Elizabeth M

Published studies show that >99% of sera reactive in the reverse syphilis testing algorithm (RSTA) screening assay with an index above an assay-specific threshold confirm as reactive, with either a rapid plasma reagin-reactive (RPR^R) or RPR-nonreactive/Treponema pallidum particle agglutination-reactive (TPPA^R) result. However, the relationship between screen indices and confirmatory patterns has not been characterized. We thus assessed confirmatory testing results for 577 sera submitted for RSTA testing and a screen-reactive result in the DiaSorin Liaison assay. The median screen index was significantly higher for RPR^R samples than TPPA^R samples (55.6 versus 10.4), and the proportion with indices >28.3 (median for all 577 samples) was significantly higher for RPR^R versus TPPA^R samples (82% versus 26%). However, RPR^R titers did not significantly correlate with screen indices (R² = 0.02). These findings demonstrate a significant relationship between RSTA screen indices and confirmatory assay results. The clinical utility of this relationship requires further study.

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