CTBP1

更新于：2025-05-07

基本信息

别名

BARS、C-terminal binding protein 1、C-terminal-binding protein 1

+ [2]

简介

Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.

关联

100 项与 CTBP1 相关的临床结果

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100 项与 CTBP1 相关的转化医学

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0 项与 CTBP1 相关的专利（医药）

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767

项与 CTBP1 相关的文献（医药）

2025-12-31·The Journal of Maternal-Fetal & Neonatal Medicine

Evaluating the healing effects of docosahexaenoic acid in neonates with bilirubin-induced brain injury

Article

作者: Shao, Peng ; Pan, Li ; Li, Yuxia ; Wang, Jun ; Tang, Yue ; Zhang, Jincan ; Liu, Peng ; Hao, Wei

BACKGROUNDNeonatal brain injury due to bilirubin toxicity presents critical need for effective healing treatments. Docosahexaenoic acid (DHA), having neuroprotective properties, offers potential therapeutic benefits in promoting brain repair and recovery.OBJECTIVESThis study focused on evaluating the healing capabilities of DHA in neonatal brains damaged by bilirubin-induced injury, with particular attention to its role in enhancing brain tissue repair mechanisms.METHODSEmploying the bilirubin encephalopathy model in neonatal Sprague-Dawley rats and neuronal cell cultures, we investigated the therapeutic impact of DHA.RESULTSThe study measured improvements in brain tissue integrity, assessed bilirubin levels, analyzed gene and protein expressions pertinent to the brain's recovery process. DHA administration resulted in significant repair in neonatal brains, evidenced by reduction in bilirubin levels and restoration of normal brain tissue architecture.CONCLUSIONMolecular analysis indicated the distinct modulation of the CTBP1/miR-155-5p/KDM5A pathway, critical for cellular repair processes and marked decrease in markers of cellular damage and stress.

2025-03-01·Cancer Genomics - Proteomics

Correlation of GD2 Biosynthesis Enzymes With Cancer Stem Cell Markers in Human Breast Cancer

Article

作者: Jiang, Wen G ; Rizk, Mariam ; Mokbel, Kefah ; O'Neill, Niamh S ; Martin, Tracey A ; Li, Amber X

BACKGROUND/AIMThe disialoganglioside GD2 has been shown to promote cell proliferation, migration, tumor and metastasis through specific signaling pathways in tumor cells originating from the neuroectoderm, including melanomas, neuroblastomas, glioblastomas, and breast carcinomas. GD2 has therefore emerged as a potential diagnostic biomarker in early malignancy as evidenced by the high specificity of its expression in tumor cells. Furthermore, recent findings show that GD2 might also act as a novel cancer stem cell (CSC) marker. Our study aimed to investigate the relationship between GD2 and 34 recognized CSC markers in human breast cancer.MATERIALS AND METHODSWe analyzed the relationship between the mRNA expression profiles of three key enzymes involved in the biosynthesis of GD2 - B4GalT5, B4GALNT1, and ST8SIA1 - and 34 CSC markers in 91 human breast cancer tissue samples.RESULTSAll three enzymes had positive and statistically significant correlation between each other with p<0.0001. Furthermore, each enzyme was found to have highly significant correlations with 15 CSC markers associated with aggressive cancer behavior: BMI1, CX43, ALCAM (CD166), Podoplanin, CD29, CD24, CD49f, IL8RA, NGFR, hTERT, Nestin, OCT4, CTBP, PSCA and Myc.CONCLUSIONThese findings lend further support to the growing evidence that GD2 is a potential biomarker of CSCs and epithelial-mesenchymal transition (EMT) in human breast cancer that can be amenable to therapeutic targeting.

2025-03-01·International Journal of Biological Macromolecules

Proteomic analysis of the nonstructural protein 2-host protein interactome reveals a novel regulatory role of SH3 domain-containing kinase-binding protein 1 in porcine reproductive and respiratory syndrome virus replication and apoptosis

Article

作者: Chen, Ruiai ; Rong, Fang ; Lv, Yadi ; Wen, Lianghai ; Dai, Guo ; Liu, Yufu ; Luo, Qiong ; Liu, Ding Xiang

Virus-host protein interaction is critical for successful completion of viral replication cycles. As the largest nonstructural protein (NSP) of porcine reproductive and respiratory syndrome virus (PRRSV), NSP2 plays multiple and critical roles in viral replication, antiviral immunity, cellular tropism and virulence. An interactome of this protein with host proteins would be instrumental in full understanding of these multifunctional roles. In this study, we report the identification of 120 NSP2-interacting host proteins by co-immunoprecipitation coupled liquid chromatography mass spectrometry, via rescuing and utilizing a recombinant PRRSV expressing an HA-tagged NSP2. By comparing and subtracting with cells infected with parental virus, a comprehensive interactome was constructed. Bioinformatics analysis revealed that these host factors are mainly involved in translation regulation, metabolism, signal transduction and innate immunity signaling pathways. Selection of five host proteins (CtBP1, CtBP2, HSPA2, PPP1CA, SH3KBP1) for further verification and characterization confirmed their interactions with NSP2 and differential effects on PRRSV replication. Intriguingly, interaction of NSP2 and SH3KBP1 led to specific cleavage of SH3KBP1, antagonizing its pro-apoptotic activity. Taken together, this study provides overarching views on the NSP2-host interactome, paving a solid foundation for functional studies of host proteins in PRRSV biology and their potential as targets for novel therapeutics development.

项与 CTBP1 相关的新闻（医药）

2024-06-11

·生物探索

Nat Genet | 清华大学刘念团队发现转座子LINE-1转录选择性激活远程基因表达

引言二十世纪五十年代，Barbara McClintock在玉米中发现一类能在基因组中移动、改变自身位置的DNA序列。当时，人们普遍认为基因在染色体上只能是固定不变的。这一惊世骇俗的发现打破了人们的认知，然而却并未获得认可。直到三十多年后，她和这些可以在基因组中移动的DNA序列终于等到了1983年诺贝尔生理学或医学奖——迟来的肯定。这些序列就是——转座子。人类基因组中有接近一半的序列由转座子构成，其中，LINE-1（Long interspersed nuclear element-1）占据了人类~17%的基因组序列，它也是目前人体内唯一活跃且具有自主逆转录转座能力的逆转录转座子。尽管在体细胞中LINE-1表达通常受到抑制，但在人和小鼠早期胚胎发育的合子基因组激活（zygotic genome activation，ZGA）过程中LINE-1却高表达。这不仅说明LINE-1的表达受到极其严格和精确的调控，而且暗示LINE-1的表达在关键生理过程具有重要作用。然而，关于LINE-1表达是如何被调控的，LINE-1在基因调控中的功能以及ZGA中的作用还知之甚少。 2024年6月7日17时，清华大学生命学院刘念团队在 Nature Genetics 期刊发表了题为：LINE-1 transcription activates long-range gene expression 的研究论文。该研究提供了第一个关于LINE-1表达调控的全基因组筛选结果，系统性的鉴定并验证了LINE-1作为增强子的功能和作用，揭示了LINE-1在长程基因激活中的广泛影响，并阐释了LINE-1转录如何帮助植入前胚胎中ZGA过程的成功进行，深化了我们对LINE-1表达在哺乳动物发育和疾病中的调节和功能作用的理解。刘念课题组长期从事逆转录转座子调控和功能研究。在之前的工作中，系统鉴定了LINE-1转座过程的调控因子（Liu et al. Nature 2018），并发现LINE-1可以作为拓扑结构域边界（Hong et al. Molecualr Cell 2024）。这项研究利用LINE-1 5'UTR驱动的报告基因在人类细胞中进行全基因组CRISPR-Cas9筛选，鉴定了171个LINE-1表达抑制因子和332个激活因子。这些调控因子参与功能多样的调控通路。有意思的是，很多的LINE-1表达调控因子与增强子调控和功能相关（KMT2D、CTBP1、METTL3、METTL14等），这提示LINE-1可能具有增强子活性。通过系统挖掘全基因组自转录活性调控区测序（STARR-seq）数据，研究团队发现LINE-1 5'UTR具有增强子特征，可以通过不依赖其位置或方向的方式激活基因表达。刘念团队进一步揭示了LINE-1在哺乳动物早期胚胎发育中的生物学功能。他们发现，LINE-1在小鼠早期2细胞阶段就开始表达，并且与minor ZGA基因（如Obox4、Zscan4等）接触并激活。抑制LINE-1转录或敲除LINE-1 RNA都能显著降低ZGA基因的表达，并阻碍小鼠胚胎发育在2-4细胞阶段。有趣的是，与恒河猴相比，人类基因组中特异存在的、进化上较年轻的LINE-1 PA1-5元件显著增强了人早期胚胎中ZGA基因的表达，说明LINE-1在基因组上的持续插入已经改变了灵长类动物ZGA基因表达的调控网络。由于LINE-1的活跃转座使其仍在人基因组中扩增，因此LINE-1可能在人类基因组中持续增添新的顺式DNA调控元件，进而影响着人类的多种疾病和发育过程。 L1的转录水平被功能多样的基因所精密调控，也决定着L1选择性地接触并激活远程基因表达的功能，影响着早期胚胎发育中的ZGA，也可能影响衰老、癌症和神经系统性疾病等（左）。L1转录激活了小鼠早期胚胎发育中的第一波转录行为（minor ZGA）（右）。（Credit: Nature Genetics）总而言之，该研究提供了功能多样的LINE-1表达调控因子，发现并验证了LINE-1 5' UTR作为增强子在早期胚胎发育阶段通过转录依赖的方式直接与ZGA基因相互作用激活基因表达。该工作为研究LINE-1的调控网络提供了丰富的资源，揭示了LINE-1表达调控的复杂性和多样性；解释了为什么LINE-1在胚胎发育过程中被激活，为理解LINE-1在基因组中的功能提供了新的视角。原文链接 https://www.nature.com/articles/s41588-024-01789-5 责编|探索君排版|探索君文章来源|“生物世界” End 往期精选围观一文读透细胞死亡(Cell Death) | 24年Cell重磅综述（长文收藏版）热文 Cell | 是什么决定了细胞的大小？热文 Nature | 2024年值得关注的七项技术热文 Nature | 自身免疫性疾病能被治愈吗？科学家们终于看到了希望热文 CRISPR技术进化史 | 24年Cell综述

基因疗法临床研究