ELOA

更新于：2025-05-07

基本信息

别名

ELOA、Elongin 110 kDa subunit、elongin A

+ [7]

简介

As part of a multisubunit complex composed of elongin BC complex (ELOB and ELOC), elongin A/ELOA, RBX1 and CUL5; polyubiquitinates monoubiquitinated POLR2A. SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).

关联

100 项与 ELOA 相关的临床结果

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100 项与 ELOA 相关的转化医学

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0 项与 ELOA 相关的专利（医药）

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项与 ELOA 相关的文献（医药）

2025-03-01·Journal of Biological Chemistry

TFII-I/GTF2I regulates globin gene expression and stress response in erythroid cells

Article

作者: Strouboulis, John ; Perez, Leonardo ; Nar, Rukiye ; Gibbons, Matthew D ; Qian, Zhijian ; Bungert, Jörg

Transcription factor TFII-I/GTF2I is ubiquitously expressed and has been shown to play a role in the differentiation of hematopoietic cells and in the response to various cellular stressors. We previously demonstrated that TFII-I acts as a repressor of adult β-globin gene transcription and positively regulates the expression of stress response proteins, including ATF3. Here we analyzed the function of TFII-I in TF-1 cells during erythroid differentiation and in response to cellular stress, including unfolded protein response, hypoxia, and oxidative stress. Ablation of TFII-I leads to mild changes in the cell cycle and proliferation of TF-1 cells. Importantly, TFII-I deficiency increased the expression of the adult β-globin gene with a concomitant reduction in the expression of the fetal γ-globin genes during erythropoietin-mediated erythroid differentiation of TF-1 cells. Furthermore, TFII-I regulates genes involved in stress response, including CHOP, Elongin A, ATF3, ATF4, and Grp78, and participates in the apoptotic response to stressors. In summary, the data provide further support for the role of TFII-I in stress response and the regulation of globin genes.

2024-04-30·The FASEB Journal

TCEB3 initiates ovarian cancer apoptosis by mediating ubiquitination and degradation of MCL‐1

Article

作者: Li, Yun ; Xu, Yingjie ; Cai, Ying ; Yang, Wen ; Huang, Masha

AbstractPlatinum resistance remains a major contributor to the poor prognosis of ovarian cancer. Anti‐apoptotic protein myeloid cell leukemia‐1 (MCL‐1) has emerged as a promising target for overcoming drug resistance, but different cancer cells utilize distinct protein degradation pathways to alter MCL‐1 level. We systematically investigated E3 ligases to identify novel candidates that mediate platinum resistance in ovarian cancer. Transcription Elongation Factor B (TCEB3) has been identified as a novel E3 ligase recognition subunit that targets MCL‐1 in the cytoplasm during platinum treatment other than its traditional function of targeting the Pol II in the nuclear compartment. TCEB3 expression is downregulated in platinum‐resistant cell lines and this low expression is associated with poor prognosis. The ubiquitination of MCL‐1 induced by TCEB3 leads to cell death in ovarian cancer. Moreover, platinum treatment increased the cytoplasm proportion of TCEB3, and the cytoplasm localization of TCEB3 is important for its targeting of MCL‐1. This study emphasizes the dual function of TCEB3 in homeostasis maintenance and in cell fate determination under different conditions, and provides a new insight into drug resistance in ovarian cancer.

2023-12-01·Nature Structural & Molecular Biology

Structure of the transcribing RNA polymerase II–Elongin complex

Article

作者: Dienemann, Christian ; Dybkov, Olexandr ; Chen, Ying ; Kokic, Goran ; Urlaub, Henning ; Cramer, Patrick

AbstractElongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to transcribing Pol II. The structures show that Elongin subunit ELOA binds the RPB2 side of Pol II and anchors the ELOB–ELOC subunit heterodimer. ELOA contains a ‘latch’ that binds between the end of the Pol II bridge helix and funnel helices, thereby inducing a conformational change near the polymerase active center. The latch is required for the elongation-stimulatory activity of Elongin, but not for Pol II binding, indicating that Elongin functions by allosterically regulating the conformational mobility of the polymerase active center. Elongin binding to Pol II is incompatible with association of the super elongation complex, PAF1 complex and RTF1, which also contain an elongation-stimulatory latch element.

项与 ELOA 相关的新闻（医药）

2022-11-28

·生物谷

Molecular Cancer: 长非编码RNA DLGAP1-AS2通过调节Trim21/ELOA/LHPP轴促进结直肠癌的发生和转移

结直肠癌(CRC)是全球第三大常见恶性肿瘤，也是全球第二大癌症相关死亡原因。结直肠癌的发病率不断增加，据估计，2020年约有190万例新发结直肠癌病例，93.5万人死亡。结直肠癌(CRC)是全球第三大常见恶性肿瘤，也是全球第二大癌症相关死亡原因。结直肠癌的发病率不断增加，据估计，2020年约有190万例新发结直肠癌病例，93.5万人死亡。CRC的分子发病机制尚未完全阐明，在提高CRC患者的生存率方面也取得了有限的成功。因此，需要不断的努力来阐明潜在的分子机制和识别新的治疗靶点。图片来源：https://doi.org/10.1186/s12943-022-01675-w 近日，来自江南大学附属医院的研究者们在Molecular Cancer杂志上发表了题为“Long noncoding RNA DLGAP1-AS2 promotes tumorigenesis and metastasis by regulating the Trim21/ELOA/LHPP axis in colorectal cancer”的文章，该研究表明DLGAP1-AS2是一种很有前途的预后生物标志物，它的发现揭示了结直肠癌分子发病机制的新维度，并为结直肠癌的治疗提供了一个新的靶点。长非编码RNA(LncRNAs)推动了研究，重点是它们作为癌基因或参与癌症发生的肿瘤抑制因子的作用。然而，大多数LncRNAs在结直肠癌(CRC)中的功能和机制仍不清楚。研究者用定量RT-PCR方法检测DLGAP1-AS2在多个结直肠癌队列中的表达。通过一系列体外和体内实验评价DLGAP1-AS2对结直肠癌生长和转移的影响。此外，通过RNA下拉、RNA免疫沉淀、RNA测序、荧光素酶测定、染色质免疫沉淀和挽救实验，揭示了DLGAP1-AS2在结直肠癌中的作用机制。研究者发现DLGAP1-AS2通过与Elongin A(Elongin A，Elongin A)的物理相互作用促进肿瘤的发生和转移，并通过促进含有21(Trim21)的三个基序(Trim21)介导的泛素化修饰和ELOA的降解来抑制其蛋白质稳定性，从而促进肿瘤的发生和转移。特别是，研究者发现DLGAP1-AS2通过抑制ELOA介导的LHPP的转录激活，从而阻断LHPP依赖的AKT信号通路，从而降低LHPP的表达。此外，研究者还证明了DLGAP1-AS2被切割和多聚腺苷化特异性因子(CPSF2)和切割刺激因子(CSTF3)结合并稳定。 CPSF2和CSTF3可提高DLGAP1-AS2在结直肠癌细胞中的稳定性图片来源：https://doi.org/10.1186/s12943-022-01675-w 综上所述，研究者证明了DLGAP1-AS2通过促进肿瘤生长和转移而在结直肠癌中作为致癌的lncRNA发挥作用。机制上，DLGAP1-AS2通过促进Trim21介导的泛素化和ELOA的降解来抑制ELOA蛋白的稳定性。研究者还证明了ELOA通过与其启动子结合来增强LHPP的转录，从而揭示了DLGAP1-AS2/Trim21/ELOA/LHPP在结直肠癌中的一个新的调节轴。此外，研究者还发现CPSF2和CSTF3与DLGAP1AS2结合并增加其稳定性。这些数据为结直肠癌的发生提供了洞察力，并表明DLGAP1-AS2是一种有前途的预后生物标志物和结直肠癌的治疗靶点（生物谷 Bioon.com）参考文献 Xue Wang et al. Long noncoding RNA DLGAP1-AS2 promotes tumorigenesis and metastasis by regulating the Trim21/ELOA/LHPP axis in colorectal cancer. Mol Cancer. 2022 Nov 14;21(1):210. doi: 10.1186/s12943-022-01675-w.

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