Article
作者: Li, Dan ; Storm, Nadia ; Freeman, Mason W ; Charles, Richelle C ; Maciorowski, Dawid ; Lemaitre, Julien ; Hudspeth, Elissa ; Qi, Wenbin ; Kobayashi, Dione T ; Hauser, Blake M ; Bhatt, Urja ; Kim, Maya ; Griffiths, Anthony ; Dai, Wenlong ; Maisonnasse, Pauline ; Vandenberghe, Luk H ; Chauhan, Ruchi ; Yaghmour, Sam ; LaRocque, Regina C ; Hérate, Cécile ; van der Werf, Sylvie ; Zabaleta, Nerea ; Chapon, Catherine ; Honko, Anna ; Johnson, Rebecca I ; Michalson, Kristofer T ; Cavarelli, Mariangela ; Sanmiguel, Julio ; Feldman, Jared ; Gallouët, Anne-Sophie ; Betts, Michael R ; McGlynn, Marion ; Le Grand, Roger ; Sheridan, Abigail ; Naninck, Thibaut ; Chichester, Jessica A ; Dyer, Cecilia D ; Pampena, M Betina ; Kahlaoui, Nidhal ; Price, Brian ; Cucalon, Allison ; Ryan, Edward T ; Estelien, Reynette ; Wilson, James M ; Zinn, Eric ; Marlin, Romain ; Knox, James J ; Diop, Cheikh ; Schmidt, Aaron G ; Contreras, Vanessa ; Hodge, Robert ; Tsong Fang, Raphaël Ho ; Ryan, Aisling ; Dereuddre-Bosquet, Nathalie
The SARS-CoV-2 pandemic has affected more than 185 million people worldwide resulting in over 4 million deaths. To contain the pandemic, there is a continued need for safe vaccines that provide durable protection at low and scalable doses and can be deployed easily. Here, AAVCOVID-1, an adeno-associated viral (AAV), spike-gene-based vaccine candidate demonstrates potent immunogenicity in mouse and non-human primates following a single injection and confers complete protection from SARS-CoV-2 challenge in macaques. Peak neutralizing antibody titers are sustained at 1 year and complemented by functional memory T cell responses. The AAVCOVID vector has no relevant pre-existing immunity in humans and does not elicit cross-reactivity to common AAVs used in gene therapy. Vector genome persistence and expression wanes following injection. The single low-dose requirement, high-yield manufacturability, and 1-month stability for storage at room temperature may make this technology well suited to support effective immunization campaigns for emerging pathogens on a global scale.