作者: Qu, Qianwei ; Cui, Wenqiang ; Zheng, Yadan ; Yuan, Zhongwei ; Li, Yanhua ; Zhu, Zhenxin ; Dong, Chunliu ; Huang, Xingyu ; Yuan, Shuguang ; Dong, Yue ; Chen, Xueying

Due to the large amount of antibiotics used for human therapy, agriculture, and even aquaculture, the emergence of multidrug-resistant Streptococcus suis (S. suis) led to serious public health threats. Antibiotic-assisted strategies have emerged as a promising approach to alleviate this crisis. Here, the polyphenolic compound gallic acid was found to enhance sulfonamides against multidrug-resistant S. suis. Mechanistic analysis revealed that gallic acid effectively disrupts the integrity and function of the cytoplasmic membrane by dissipating the proton motive force of bacteria. Moreover, we found that gallic acid regulates the expression of dihydrofolate reductase, which in turn inhibits tetrahydrofolate synthesis. As a result of polypharmacology, gallic acid can fully restore sulfadiazine sodium activity in the animal infection model without any drug resistances. Our findings provide an insightful view into the threats of antibiotic resistance. It could become a promising strategy to resolve this crisis.

2020-12-01·Talanta2区 · 化学

Exploration of synthesizing fluorescent silicon nanoparticles and label-free detection of sulfadiazine sodium

2区 · 化学

Article

作者: Feng, Feng ; Bai, Yunfeng ; Su, Qian ; Yang, Xiaoming ; Xiao, Jimei

Herein, silica nanoparticles (SiNPs) with blue-fluorescence have been originally synthesized through one facile hydrothermal way, and this kind of SiNPs were water-soluble with the relative quantum yield of around 6%. Meanwhile, N-(triethoxysilylpropyl) urea severed as the silica source, while potassium hydrogen phthalate as the doping reagent. Also, SiNPs exhibited the acceptable stability and excitation-dependent fluorescence property. Moreover, their surfaces of the obtained SiNPs were equipped with multiple functional groups including -Si-O-Si-, -Si-H, -COOH, -NH₂ and -OH. Importantly, the fluorescence of SiNPs could be specifically quenched by sulfadiazine sodium (SD-Na), thus achieving a label-free detection of SD-Na, which displayed a wide linear response in the range of 0.8 μM-800 μM with a detection limit of 1.02 μM. Additionally, we explored the mechanism of SiNPs sensing SD-Na on the basis of aggregation-induced quenching. To be specific, the particle size of SiNPs increased from 29.9 nm to 203.7 nm induced by the electrostatic interactions between SiNPs and SD-Na, which was further confirmed by high resolution transmission electron microscopy. Consequently, the proposed strategy here broadened the ways of assaying sulfadiazine sodium.

2019-10-01·Antimicrobial Agents and Chemotherapy2区 · 医学

Sulfadiazine Sodium Ameliorates the Metabolomic Perturbation in Mice Infected with Toxoplasma gondii

2区 · 医学

Article

作者: Zhou, Chun-Xue ; Zhu, Xing-Quan ; Cong, Hua ; Gan, Yun ; Elsheikha, Hany M. ; Chen, Xiao-Qing ; Liu, Qing

In this study, we analyzed the global metabolomic changes associated withToxoplasma gondiiinfection in mice in the presence or absence of sulfadiazine sodium (SDZ) treatment. BALB/c mice were infected withT. gondiiGT1 strain and treated orally with SDZ (250 μg/ml in water) for 12 consecutive days. Mice showed typical manifestations of illness at 20 days postinfection (dpi); by 30 dpi, 20% had survived and developed latent infection.

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