作者: Zheng, Liping ; Zhao, Han ; Zhou, Xiumei ; Zhu, Yingwei ; Fang, Hongming ; Xia, Pengguo ; Qin, Yuan ; Wang, Yigang ; Chen, Xindong ; Xiang, Zhongyi ; Huang, Biao ; Hong, Jianfeng

To establish a rapid and highly sensitive assay for tumor-associated trypsinogen-2 (TAT-2) based on the time-resolved fluorescence immunoassay (TRFIA) and evaluate its potential clinical value in patients with lung cancer. The double-antibody sandwich method was used in detecting TAT-2 antigen concentrations, and two types of TAT-2 antibodies (coating antibodies and Eu³⁺ labeled antibodies) were used. A TAT-2-TRFIA method was then established, evaluated, and used in detecting the serum TAT-2 levels of healthy subjects and patients with lung cancer. The linear range of the TAT-2-TRFIA method was 1.53-300 ng/mL, the intra-assay coefficient of variation (CV) were between 1.67% and 8.42%, and the inter-assay CV were between 4.29% and 11.44%. The recovery rates of TAT-2-TRFIA were between 99.17% and 107.06%. The cross-reactivities of trypsin and T-cell immunoglobulin mucin 3 were 0.02% and 0.82%, respectively. The serum TAT-2 levels of patients with lung cancer were higher than those of healthy subjects (P < 0.001). Combined with TAT-2, the sensitivity and specificity of CEA and CA-125 for lung cancer improved significantly. Conclusion: We successfully established a highly sensitive TAT-2-TRFIA method, which was able to facilitate the timely diagnosis of lung cancer.

2016-04-01·Asian Pacific journal of tropical medicine4区 · 医学

In vivo anticancer activity of maesopsin 4- O - β -glucoside isolated from leaves of Artocarpus tonkinensis A. Chev. Ex Gagnep

4区 · 医学

ArticleOA

作者: Thao, Do Thi ; Delfino, Domenico V ; Anh, Nguyen Thi Hoang ; Mai, Le Phuong ; Thien, Dao Duc ; Van Sung, Tran ; Thuy, Trinh Thi ; Tam, Nguyen Thanh ; Nga, Nguyen Thi ; Cuc, Nguyen Thi ; Quang Hung, Tran

OBJECTIVE:

To investigate the antitumor effect of maesopsin 4-O-β-glucoside (TAT2) isolated from the leaves of Artocarpus tonkinensis (A. tonkinensis) A. Chev. ex Gagnep.

METHODS:

The antitumor activity of TAT2 was evaluated in Lewis lung carcinoma (LLC) tumor-bearing mice. BALB/c mice had tumors induced by implantation with 2 × 10(6) LLC cells into the subcutaneous right posterior flank. Tumor-bearing mice were treated orally with a range of doses of TAT2 and a standard drug, doxorubicin. Animals were observed for tumor growth and mortality rate. Blood was collected to determine hematological and biochemical parameters.

RESULTS:

TAT2 was isolated from an ethanolic extract of A. tonkinensis leaves. Its structure was determined by MS and NMR spectroscopy, and identified as TAT2. The compound did not show acute toxicity at the highest dose tested (2000 mg/kg body weight). TAT2 exhibited antitumor activity by decreasing tumor growth, increasing the survival rate, and ameliorating some hematological and biochemical parameters at doses of 100 and 200 mg/kg body weight (P < 0.05).

CONCLUSIONS:

These results indicate that TAT2 possesses clear antitumor activity. Due to its bioavailability and low toxicity, and the fact that it could be isolated in a large scale from A. tonkinensis leaves, the compound shows promise as a potential anticancer drug.

2011-06-01·Journal of chemotherapy (Florence, Italy)4区 · 医学

Maesopsin 4-O-β-_D-Glucoside, a Natural Compound Isolated from the Leaves ofArtocarpus tonkinensis, Inhibits Proliferation and Up-Regulates HMOX1, SRXN1 and BCAS3 in Acute Myeloid Leukemia

4区 · 医学

Article

作者: Pierangeli, S. ; Pettorossi, V. ; Liberati, A. M. ; Pozzesi, N. ; Thuy, T. T. ; Sung, T. V. ; Ninh, P. T. ; Delfino, D. V. ; Vacca, C. ; Falchi, L. ; Martelli, M. P. ; Riccardi, C.

The leaves of Artocarpus tonkinensis are used in Vietnamese traditional medicine for treatment of arthritis, and the compound maesopsin 4-O-β-D-glucoside (TAT-2), isolated from them, inhibits the proliferation of activated T cells. Our goal was to test the anti-proliferative activity of TAT-2 on the T-cell leukemia, Jurkat, and on the acute myeloid leukemia, OCI-AML. TAT-2 inhibited the growth of OCI-AML (and additional acute myeloid leukemia cells) but not Jurkat cells. Growth inhibition was shown to be due to inhibition of proliferation rather than increase in cell death. Analysis of cytokine release showed that TAT-2 stimulated the release of TGF-β, yet TGF-β neutralization did not reverse the maesopsin-dependent effect. Gene expression profiling determined that maesopsin modulated 19 identifiable genes. Transcription factor CP2 was the gene most significantly modulated. Real-time PCR validated that up-regulation of sulphiredoxin 1 homolog (SRXN1), hemeoxygenase 1 (HMOX1), and breast carcinoma amplified sequence 3 (BCAS3) were consistently modulated.

100 项与 TAT-2 相关的药物交易

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