Bioactivity profiling of Sanghuangporus lonicerinus : antioxidant, hypoglycaemic, and anticancer potential via in-vitro and in-silico approaches

Article

作者: Abdullaev, Ikram ; Petri, Edward ; Gafforov, Yusufjon ; Ghosh, Soumya ; Lim, Young Won ; Yarasheva, Manzura ; Rašeta, Milena ; Živanović, Nemanja ; Bekić, Sofija ; Chen, Jia Jia ; Abbasi, Arshad Mehmood ; Mišković, Jovana ; Wan-Mohtar, Wan Abd Al Qadr Imad ; Rapior, Sylvie ; Abdullaev, Bekhzod

This study investigates the mycochemical profile and biological activities of hydroethanolic (EtOH), chloroform (CHCl₃), and hot water (H₂O) extracts of Sanghuangporus lonicerinus from Uzbekistan. Antioxidant capacity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), NO, and FRAP assays, and in vitro hypoglycaemic effects were evaluated through α-amylase and α-glucosidase inhibition. Antiproliferative potential was explored by analysing the binding affinities of EtOH and H₂O extracts to estrogen receptor α (ERα), ERβ, androgen receptor (AR), and glucocorticoid receptor (GR), with molecular docking providing structural insights. LC-MS/MS analysis revealed solvent-dependent phenolic profiles, with the EtOH extract containing the highest total phenolic content (143.15 ± 6.70 mg GAE/g d.w.) and the best antioxidant capacity. The EtOH extract showed significant hypoglycaemic effects, with 85.29 ± 5.58% inhibition of α-glucosidase and 41.21 ± 0.79% inhibition of α-amylase. Moderate ERβ binding suggests potential for estrogen-mediated cancer therapy, while strong AKR1C3 inhibition by the EtOH extract supports its therapeutic potential.

2025-10-01·Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

Natural products as probes for the spectral analysis and sensitive detection of metal-containing ionic liquids

Article

作者: Feng, Xueting ; Yao, Shun ; Mahmood, Subhan ; Nie, Lirong ; Li, Xinlu ; Zhou, Gaojin

A new analytical method was developed for the rapid detection of metal-containing ionic liquids (MCILs) using the natural products (NPs) as fluorescent probes. Based on electrostatic potential (ESP) distribution and conductor-like screening model for real solvents (COSMO-RS) simulation, the possible interactions between quercetin, emodin, curcumin, esculetin and 1-butyl-3-methylimidazole tetrachloroferrate ([Bmim][FeCl₄]) were predicted, and the ultraviolet response, concentration titration experiments and fluorescence response of NPs and [Bmim][FeCl₄] were studied. Both theoretical and experimental studies indicated that esculetin showed the best corresponding performance to [Bmim][FeCl₄] and then was selected as fluorescent probe molecule for the detection of a series of similar ionic liquids containing Zn²⁺, Cu²⁺ and Al³⁺. The linear range of this analytical method towards [Bmim][FeCl₄] was 5 ∼ 30 μM (R² = 0.9957) with the relative standard deviation of 2.1 % and the limit of detection (LOD) of 1.08 μM. Recoveries, measured in the standard ethanol solution with the added concentration of 5 μM, were determined as 98.1 ∼ 102.5 % by the standard addition method. According to all the studied results, the probe of natural product possessed good selectivity, repeatability, and stability, which could be fixed onto filter paper to detect [Bmim][FeCl₄] in more intuitive way.

2025-07-01·Journal of Pharmaceutical and Biomedical Analysis

An integrated analysis strategy for characterization of chromones and coumarins from Saposhnikovia divaricata (Turcz.) Schischk. by UHPLC-QTOF-MS

Article

作者: Kuang, Hai-Xue ; Hao, Zhi-Chao ; Liu, Shu ; Sun, Yan ; Sui, Yao-Xin ; Zhang, Li-Li ; Li, Yan-Ying ; Chen, Zhi-Jiang ; Jiang, Peng ; Guan, Wei ; Yang, Bing-You ; Wang, Yu-Qing ; Liu, Si-Tong ; Chen, Qing-Shan ; Liu, Yan

This study aimed to employ a comprehensive data screening strategy to identify the chromones and coumarins present in Saposhnikovia divaricata (SD). Initially, the five-point mass defect filter (MDF) method was utilized to screen the respective three subclasses of chromones and coumarins for MS¹. In comparison to the traditional MDF method, the number of interference peaks was reduced from 3462 to 1053, representing a decrease of 69.58 %. Then, diagnostic fragment ion filtering (DFIF) was used to screen product ions selected by MDF method, which was based on the fragmentation rules of each subclass to screen whether it met the conditions. Finally, we characterized 94 compounds from SD, including 40 chromones and 54 coumarins, among them, 82 chromones and coumarins were identified by combining the above two methods, 12 coumarins were identified by combining MDF and references, they were classified into other coumarins. Twenty one compounds were identified for the first time from SD, and 3 chromones and 7 coumarins were unknown compounds. Through untargeted metabolomics analysis of SD samples from 12 different regions, significant differences were found in SD samples from different areas, and 20 differential metabolites were distinguished, including 13 chromones and 7 coumarins. This study established for the first time a comprehensive strategy combining MDF, DFIF, and untargeted metabolomics to evaluate SD quality. The results indicated that this method is an efficient, accurate, and promising approach for classifying and exploring compounds in complex natural product systems, providing a basis for evaluating the quality of SD from different sources.

100 项与 Esculetin 相关的药物交易

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适应症	最高研发状态	国家/地区	公司	日期
动脉粥样硬化	临床前	-	CSIR-Indian Institute of Chemical Biology	2024-05-17
动脉粥样硬化	临床前	-	Academy of Scientific & Innovative Research	2024-05-17