作者: Hanway, Tim R. ; Gillespie, Jonathan C. ; Serrano, Eric R. ; Hodges, Falyn L. ; Wallace, William A. ; Raub, Betsy J. ; Moon, Jordan R. ; McCoun, Julie A.

Tocotrienols and tocopherols are lipid‐soluble chemicals that make up vitamin E. Vitamin E can be subcategorized as (α‐tocopherol, β‐tocopherol, γ‐tocopherol, and δ‐tocopherol) and four primary tocotrienols (α‐tocotrienol, β‐tocotrienol, γ‐tocotrienol, δ‐tocotrienol). Tocotrienols receive less recognition for their effects on health compared to their counterparts, tocopherols. The purpose of this study was to investigate the pharmacokinetics and bioavailability of a powdered, sublingual form of DT3 in healthy men and women. We hypothesized that there would be an increase in plasma DT3 followed by a decrease after reaching peak concentration over an 8‐hour period. Volunteers (m=1, f=4; age: 36.6 +/‐ 10 years, 169 +/‐ 6 cm, 77 +/‐ 14.6 kg) were administered a 40mg of DT3 powder after fasting for 8 hours and given a meal consisting of carbohydrates, proteins, and fats after 4 hours. Volunteers were asked to keep the powder under their tongues for 30 seconds or until the powder was completely dissolved. Blood samples were collected at 0, 30, 45, 60, 90, 120, 180, 240, 360, and 480 minutes. Blood samples collected were placed in the centrifuge to separate plasma from blood. The plasma collected was stored at ‐80°F in a cryogenic freezer. DT3 concentrations were measured from plasma using protein precipitation followed by liquid‐liquid extraction. Analytes were separated by HPLC with the extracts assayed against a calibration curve. DT3 bioavailability was assessed using the parameters of peak plasma concentration (Cmax), time to reach peak plasma concentration (Tmax) and total area under the plasma concentration‐time curve (AUC). Plasma concentration of DT3 reached 47.1 ng/ml (Cmax) 360 minutes after administration (Tmax). DT3 concentration decreased between 360 and 480 minutes. The 0‐8 h AUC reached 10,093 ng/ml*min. Ingesting a 40mg dose of DT3 dissolved orally and then swallowed appears to produce a slow progressive upward absorption trend over the time span of 6 hours after. Following the 6‐hour increase in DT3 concentration a subsequent decrease in plasma DT3 was observed. Optimal dosing and timing of DT3 when dissolved in the mouth may enhance the efficacy of δ‐tocotrienols on health.

2003-01-01·Neuroscience letters4区 · 医学

Vitamin E isoforms α-tocotrienol and γ-tocopherol prevent cerebral infarction in mice

4区 · 医学

Article

作者: Kazuhisa Matsunaga ; Katsunori Iwasaki ; Michihiro Fujiwara ; Jiro Takata ; Nobuaki Egashira ; Kenichi Mishima ; Yoshiharu Karube ; Takamitsu Tanaka ; Ryoji Hidaka ; Fengling Pu

Alpha-tocopherol and its derivatives have been shown to be effective in reducing cerebral ischemia-induced brain damage. However, the effects of other vitamin E isoforms have not been characterized. In the present study, we investigated the effects of six different isoforms of vitamin E on the ischemic brain damage in the mice middle cerebral artery (MCA) occlusion model. All vitamin E isoforms were injected i.v., twice, immediately before and 3 h after the occlusion. Alpha-tocopherol (2 mM), alpha-tocotrienol (0.2 and 2 mM) and gamma-tocopherol (0.2 and 2 mM) significantly decreased the size of the cerebral infarcts 1 day after the MCA occlusion, while gamma-tocotrienol, delta-tocopherol and delta-tocotrienol showed no effect on the cerebral infarcts. These results suggest that alpha-tocotrienol and gamma-tocopherol are potent and effective agents for preventing cerebral infarction induced by MCA occlusion.

MOLECULES

Bioaccessibility of Tocols in Commercial Maize Hybrids Determined by an In Vitro Digestion Model for Poultry

Article

作者: Pirgozliev, Vasil ; Kljak, Kristina ; Međimurec, Tatjana ; Kiš, Goran ; Grbeša, Darko ; Zurak, Dora ; Gunjević, Veronika

Despite the high proportion of maize grain in animal diets, the contribution made by maize phytochemicals is neglected. Tocols and their contribution to the vitamin E content of animal diets are one example, exacerbated by sparse information on the tocol bioaccessibility of commercial hybrids. In this study, the contents of individual and total tocols and their bioaccessibility were determined in the grain samples of 103 commercial hybrids using a standardized INFOGEST digestion procedure. In the studied hybrids, total tocol content ranged from 19.24 to 54.44 µg/g of dry matter. The contents of micellar α-, γ-, δ-tocopherols, γ-tocotrienol, and total tocols correlated positively with the corresponding contents in the grain samples of the studied hybrids. In contrast, a negative correlation was observed between the bioaccessibility of γ- tocopherol, α- and γ-tocotrienol, and total tocols, along with the corresponding contents in the grain of studied hybrids. The highest bioaccessibility was exhibited by γ-tocotrienol (532.77 g/kg), followed by δ-tocopherol (529.88 g/kg), γ-tocopherol (461.76 g/kg), α-tocopherol (406.49 g/kg), and α-tocotrienol (359.07 g/kg). Overall, there are significant differences in the content and bioaccessibility of total and individual tocols among commercial maize hybrids, allowing the selection of hybrids for animal production based not only on crude chemical composition but also on the content of phytochemicals.

100 项与 β-Tocotrienol 相关的药物交易

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