Vitamin D receptors are expressed in activated different immune cells. It is not known, which immune cell type is targeted by exogenous vitamin D. Here, vitamin D-deficient individuals will receive once 100.000 I.U. vitamin D3 either intramuscular or subcutaneous in a double-blind placebo controlled setting. Immune cells will be monitored from the blood over time.

开始日期2013-02-01

申办/合作机构

Charité - Universitätsmedizin Berlin

100 项与胆维丁相关的临床结果

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100 项与胆维丁相关的转化医学

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100 项与胆维丁相关的专利（医药）

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项与胆维丁相关的文献（医药）

2024-04-01·European Journal of Lipid Science and Technology

Previtamin D₂, vitamin D₂, and vitamin D₄ amounts in different mushroom species irradiated with ultraviolet (UV) light and occurrence of structurally related photoproducts

作者: Sommer, Katrin ; Hillinger, Marissa ; Vetter, Walter

AbstractMushrooms are rich in ergosterol and ergosta‐5,7‐dienol, which can be partly converted into vitamin D2 and D4 through ultraviolet (UV) light exposure. Typically, mushrooms have very low vitamin D contents, but it can be increased by UV irradiation. This process generates additional photoisomers scarcely studied in mushrooms due to analytical challenges. Here, we developed a new solid phase extraction (SPE) method to separate vitamin D2, vitamin D4, and other tri‐ and pentacyclic photoisomers from the much higher abundant ergosterol. Subsequent GC/MS analysis enabled the detection of ten photoisomers in eight UV‐treated mushroom species, including vitamin D2 (previtamin D2, tachysterol2, two suprasterol2 and trans‐vitamin D2 isomers) and vitamin D4 (previtamin D4). Quantitated vitamin D2 contents of 10–540 µg/100 g dry weight agreed well with the sparse literature data available for the investigated mushroom species. In addition, previtamin D2 (nd–1950 µg/100 g dry weight) and vitamin D4 (10–140 µg/100 g dw) were quantified in the samples. The content and photoproduct compositions varied considerably between different mushroom species.Practical applications: The novel SPE method can be applied to study the vitamin D and photoisomer content of mushrooms.

2021-01-01·Problems of Biological Medical and Pharmaceutical Chemistry

Vitamin D and its status in vegetarians and vegans

作者: Ranjit, R. ; Galchenko, A.V.

Vitamin D is an essential hydrophobic micronutrient.There are at least seven different forms of vitamin D, however, only D₂ and D³ are considered to be its biol. active forms in the human body.Besides its primary functions of calcium homeostasis and bone metabolism, vitamin D exerts a number of addnl. biol. effects.For instance, it is a powerful immunomodulator.Lack of vitamin D is primarily associated with osteoporosis and rickets.Moreover, vitamin D deficiency can aggravate the risks of cardiovascular diseases, autoimmune diseases, and even tumor genesis.At the same time, vitamin D can be extremely toxic if taken in excess.As a consequence of vitamin D hypervitaminosis, severe impairment in calcium metabolism may occur and can result in neuropsychiatric abnormalities, gastrointestinal, renal, or cardiovascular disorders.The human body mainly obtains vitamin D₃ by endogenously biosynthesizing it from cholesterol in the skin in presence of UV radiation.The richest food source of vitamin D₃ is oily sea fish.Much smaller amounts of calciferol are found in dairy products, eggs, or liver.Mushrooms are also capable of accumulating vitamin D under the influence of sunlight, but they store vitamin D in its less active form - vitamin D₂.Despite the presence of different forms of vitamin D in various foods, it is extremely difficult to compensate for its need if relied only on food, even for people who regularly include fish in their diet.And while vegetarians and vegans consume significantly less vitamin D and generally have lower serum concentrations, the entire population of extratropical regions is highly susceptible to calciferol deficiency, regardless of their diet.Therefore, it seems reasonable to regularly screen the vitamin D supply, especially for people living in high latitudes, no matter which diet they follow.

2018-04-01·World Journal of Microbiology and Biotechnology4区 · 工程技术

Rate-limiting steps in the Saccharomyces cerevisiae ergosterol pathway: towards improved ergosta-5,7-dien-3β-ol accumulation by metabolic engineering

4区 · 工程技术

Article

作者: Tang, Xiao-Ling ; Zheng, Ren-Chao ; Ke, Xia ; Ma, Bin-Xiang ; Zheng, Yu-Guo

Ergosterol is the predominant nature sterol constituent of plasma membrane in Saccharomyces cerevisiae. Herein, the biosynthetic pathway of ergosterol was proposed to be metabolically engineered for the efficient production of ergosta-5,7-dien-3β-ol, which is the precursor of vitamin D4. By target disruption of erg5, involved in the end-steps of post-squalene formation, predominantly accumulated ergosta-5,7-dien-3β-ol (4.12 mg/g dry cell weight). Moreover, the rate-limiting enzymes of ergosta-5,7-dien-3β-ol biosynthesis were characterized. Overexpression of Hmg1p led to a significant accumulation of squalene, and induction of Erg1p/Erg11p expression raised the yield of both total sterols and ergosta-5,7-dien-3β-ol with no obvious changes in growth behavior. Furthermore, the transcription factor allele upc2-1 was overexpressed to explore the effect of combined induction of rate-limiting enzymes. Compared with an obviously enhanced yield of ergosterol in the wild-type strain, decreases of both the ergosta-5,7-dienol levels and the total sterol yield were found in Δerg5-upc2-1, probably due to the unbalanced NADH/NAD⁺ ratio observed in the erg5 knockouts, suggesting the whole-cell redox homeostasis was also vital for end-product biosynthesis. The data obtained in this study can be used as reference values for the production of sterol-related intermediates involved in the post-squalene biosynthetic pathway in food-grade S. cerevisiae strains.

100 项与胆维丁相关的药物交易

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