Formaldehyde, as a frequently used compound in many applications, crosses the blood‐brain barrier and leads to hippocampal cell death and memory impairment. This study investigates the effects of ethanolic extract of Matricaria chamomilla (MC) on passive avoidance learning induced by damaged hippocampal cells and evaluates the antioxidant traits of MC. The male Wistar rats were divided into six (6 in each) groups: control (10 mg/kg normal saline), 200 (200 mg/kg MC extract), 500 (500 mg/kg MC extract), F (10 mg/kg formaldehyde), F200 (10 mg/kg formaldehyde and 200 mg/kg MC extract), and F500 (10 mg/kg formaldehyde and 500 mg/kg MC extract). Shuttle box assay was used for evaluation of passive avoidance learning. The apoptosis rate of hippocampal tissue, malondialdehyde (MDA) free radicals, and total antioxidant capacity was evaluated to determine the positive effect of the ethanolic extract of MC. We found that the ethanolic extract of MC reduced the cell death, time spent in a dark room, and MDA free radicals in the hippocampus, leading to increased total antioxidant capacity in this region. In conclusion, the ethanolic extract of MC could ameliorate formaldehyde‐induced memory damage through decreasing cell death and MDA activity of the hippocampal region and increasing total antioxidant capacity.

2016-03-01·Theriogenology2区 · 农林科学

Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle

2区 · 农林科学

Article

作者: Baruselli, P S ; Vieira, L M ; Rodrigues, C A ; Marques, K N G ; Sá Filho, M F ; Castro Netto, A ; Mapletoft, R J ; Bó, G A ; Silveira, C R A ; Bragança, L G M ; Freitas, B G ; Guerreiro, B M

Plasma FSH profiles, in vitro embryo production (IVP) after ovum pickup (OPU), and establishment of pregnancy with IVP embryos were compared in untreated Holstein oocyte donors and those superstimulated with multiple injections or a single intramuscular (IM) injection of porcine FSH (pFSH) in hyaluronan (HA). Plasma FSH profiles were determined in 23 heifers randomly allocated to one of four groups. Controls received no treatment, whereas the F200 group received 200 mg of pFSH in four doses, 12 hours apart. The F200HA and F300HA groups received 200- or 300-mg pFSH in 5 mL or 7.5 mL, respectively of a 0.5% HA solution by a single IM injection. Plasma FSH levels were determined before the first pFSH treatment and every 6 hours over 96 hours. All data were analyzed by orthogonal contrasts. Circulating FSH area under curve (AUC) in pFSH-treated animals was greater than that in the control group (P = 0.02). Although the AUC did not differ among FSH-treated groups (P = 0.56), the total period with elevated plasma FSH was greater in the F200 group than in the HA groups (P < 0.0001). However, the F300HA group had a greater AUC than the F200HA group (P = 0.006), with a similar total period with elevated plasma FSH (P = 0.17). The IVP was performed in 90 nonlactating Holstein cows randomly allocated to one of the four treatment groups as in the first experiment. A greater proportion of medium-sized (6-10 mm) follicles was observed in cows receiving pFSH, regardless of the treatment group (P < 0.0001). Also, numbers of follicles (P = 0.01), cumulus-oocyte complexes (COCs) retrieved (P = 0.01) and matured (P = 0.02), cleavage rates (P = 0.002), and blastocysts produced per OPU session (P = 0.06) were greater in cows receiving pFSH, regardless of the treatment group. Cows in the F200HA group had a greater recovery rate (P = 0.009), number of COCs cultured (P = 0.04), and blastocysts produced per OPU session (P = 0.06) than cows in the F300HA group. Similar pregnancy rates were observed 50 to 60 days after transferring IVP embryos from donors in the different treatment groups (P > 0.05). In conclusion, a single IM injection of pFSH combined in 0.5% HA resulted in similar plasma FSH profiles as twice-daily pFSH treatments. Treatment of nonlactating donors with pFSH, with or without HA, resulted in increased IVP over untreated controls. A single dose of 200 mg of pFSH in 0.5% HA resulted in greater IVP than 300-mg pFSH in HA. Finally, pregnancy rates with IVP embryos were similar, regardless donor treatment.

2009-04-01·Journal of Biomedical Materials Research Part B: Applied Biomaterials3区 · 工程技术

Cell adhesion and proliferation are reduced on stainless steel coated with polysaccharide‐based polymeric formulations

3区 · 工程技术

Article

作者: Pasquale P. Vicario ; Zichun Lu ; Tom Schottman ; Irina Grigorian ; Zheng Wang

AbstractHydromer's polymeric formulations F200™ and F202™ were evaluated after application to a synthetic substrate for effects on cell adhesion and proliferation. A significant reduction in cell adhesion was observed when cells grown on medical‐grade stainless steel coated with these polymers were stained and examined under a fluorescence microscope. This reduction in cell adhesion/proliferation was confirmed when cells were isolated and analyzed by the MTS cell proliferation assay. The rate of growth of cells on F200‐ and F202‐coated stainless steel monitored over a period of 7 days was significantly less than that observed on uncoated stainless steel, suggesting that the rate of growth of cells was reduced. The adhesion/proliferation of human umbilical vein endothelial cells (HUVEC) to coated substrates was also decreased significantly, indicating that the reduction in cell adhesion/proliferation is not restricted to only fibroblasts. Additional studies have indicated that the adhesion/proliferation of murine fibroblasts and human endothelial cells to stainless coated with a modified formulation exhibiting a high degree of lubricity was also significantly reduced. This lubricious formulation was also observed to be effective in reducing platelet adhesion, data supporting the view that lubricity also contributes to a reduction in cell and platelet adhesion. The application of these polymeric coatings on devices designed for medical implantation may not only prevent thrombus formation but may also retard the process of restenosis. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009

100 项与 F-200 (Shanghai Genomics) 相关的药物交易

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