Introduction:More than 20 protozoan species of Leishmania are responsible for
causing Leishmaniasis, an infection spread by blood-feeding phlebotomine sandflies. A narrow
pool of drugs is currently available rendering the current drug stratagem to treat this infection .
Development of novel, less toxic, and more effective regimens is thus a need of the hour. Design
and synthesis of benzo[d]imidazole carboxamides as agents to combat Leishmaniasis are
also required.Methods:14 benzo[d]imidazole carboxamides were synthesized and gauged against L. donovani
promastigotes and intramacrophage amastigote forms. All of the tested compounds exhibited
significant anti-promastigote properties with IC50 well below 10 uM. Compounds 4a,
4b, and 4d, showing the highest anti-parasitic activity against promastigote forms (IC50 0.91-
1.33 μM), were also found to be associated with better anti-leishmanial potential (IC50 0.78-
1.67 μM) against the intramacrophage amastigotes comparable to Amphotericin-B (0.13 μM),
a drug used for Leishmaniasis. Compound (4a), namely N-(2-(trifluoromethyl)-1Hbenzo[
d]imidazol-5-yl)benzo[d][1,3]-5-carboxamide-dioxole, was found to be most potent
against L. donovani amastigotes among all the tested compounds, and demonstrated better antileishmanial
properties (IC50 0.78 μM) when compared to the standard. Compound 4a was also
assessed for its toxicity profile against THP-1 human monocytic cells. To establish the molecular
target(s) in silico, molecular docking studies were performed against cysteine protease,
a putative virulence factor of Leishmania parasites, and nucleoside diphosphate kinase, an enzyme
with a critical role in nucleotide recycling, also associated with resistance in Leishmania
strains. Compound 4a showed better binding affinity than the standard to these targets; furthermore,
the molecular dynamic simulation studies further affirmed the stability of compound
4a, within the active site of the targets. In vitro, cysteine protease inhibitory activity
(IC50 8.53 μM) using Bz-Arg-AMC hydrochloride fluorogenic peptide substrate established the
promising potential of 4a as a cysteine protease inhibitor.Result:Computational ADMET analysis indicated appropriate pharmacokinetic profile and
physicochemical characteristics for all members of the synthesized library.Conclusion:Both in vitro and in silico studies indicate that the synthesized imidazole carboxamides
can act as potent hits and that N-(2-(trifluoromethyl)-1H-benzo[d]imidazol-5-
yl)benzo[d][1,3]-5-carboxamide-dioxole 4a can be an effective hit molecule which can be further
developed into potent lead molecule (s) to fight Leishmania donovani.