Article
作者: Kumar, Ashok ; Gulati, Baldev R ; Tripathi, Bhupendra N ; Kumar, Ram ; Yousuf, Raja Wasim ; Bashir, Arif ; Kachhawaha, Subhash ; Barua, Sanjay ; Verma, Assim ; Dutt, Triveni ; Yadav, Vijaypal ; Tiwari, Hari Audh ; Vyas, Vithilesh ; Khandelwal, Nitin ; Riyesh, Thachamvally ; Munjal, Vijay Kumar ; Singh, Lokender ; Kumar, Amit ; Andrabi, Anjum ; Koul, Abhimanyu ; Chaudhary, Sarjeet ; Pathak, Anubha ; Gurav, Amol ; Bhardwaj, Bhupendra ; Nandi, Sukdeb ; Gupta, Madhurendu K ; Kumar, Sanjay ; Misri, Jyoti ; Gaur, Mitesh ; Bhati, Adrish ; Singh, Karam Pal ; Kaul, Rakesh ; Kumar, Naveen ; Godara, Bhagraj ; Pal, Yash ; Mehta, Vishal ; Kumar, Rajender ; Gautam, Siddharth ; Kumar, Garvit ; Chander, Yogesh ; Dedar, Ramesh Kumar ; Sharma, Deepak Kumar ; Mohanty, Ashok Kumar
Lumpy skin disease (LSD) was reported for the first time in India in 2019 and since then, it has become endemic. Since a homologous (LSD-virus based) vaccine was not available in the country, goatpox virus (GPV)-based heterologous vaccine was authorized for mass immunization to induce protection against LSD in cattle. This study describes the evaluation of safety, immunogenicity and efficacy of a new live-attenuated LSD vaccine developed by using an Indian field strain, isolated in 2019 from cattle. The virus was attenuated by continuous passage (P = 50) in Vero cells. The vaccine (50th LSDV passage in Vero cells, named as Lumpi-ProVacInd) did not induce any local or systemic reaction upon its experimental inoculation in calves (n = 10). At day 30 post-vaccination (pv), the vaccinated animals were shown to develop antibody- and cell-mediated immune responses and exhibited complete protection upon virulent LSDV challenge. A minimum Neethling response (0.018% animals; 5 out of 26,940 animals) of the vaccine was observed in the field trials conducted in 26,940 animals. There was no significant reduction in the milk yield in lactating animals (n = 10108), besides there was no abortion or any other reproductive disorder in the pregnant animals (n = 2889). Sero-conversion was observed in 85.18% animals in the field by day 30 pv.