2012-07-01·European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery3区 · 医学

Tissue repair driven by two different mechanisms of growth factor plasmids VEGF and NGF in mice auricular cartilage: regeneration mediated by administering growth factor plasmids

3区 · 医学

Article

作者: Daniela Pinterova ; Martin Cegan ; Katarina Kolostova ; Lenka Ceganova ; Vladimir Bobek ; Oliver Taltynov ; Marie Jirkovska

The focus of this study was to compare the role of nerve growth factor (NGF) and vascular endothelial growth factor (VEGF) in the regeneration of experimental skin and cartilage trauma. The role of VEGF in this process is known since decade; the NGF participation on this process has been first discussed within the spinal cord injury repair. We hypothesized that both VEGF and NGF induce angiogenesis and take part on the repair process. The angiogenesis response and the cartilage regeneration after phVEGF(165) plasmid and rat pcNGF plasmid administration were investigated using BALB/c mice. PhVEGF(165) and pcNFG were injected into the right mice ear and plain vector injection into the left ear the day before trauma. The next day, all mice were ear-punched, resulting in 2-mm diameter puncture through the center of both pinnae. In BALB/c mouse strain, a significantly faster cartilage repair was observed after phVEGF(165) and pcNGF injection into punched ear area in comparison to the control group. It has been shown that the healing process is after VEGF and NGF injection driven differentially. In case of VEGF is the cartilage wound repaired by induction of new chondrocytes differentiation. In the case of NGF, the regeneration is supported by immature leukocytes attracted into the punched area. The leukocytes induct angiogenesis so far indirectly by inflammation. The NGF-induced inflammation environment may be a part of mosaic creating the complete picture of regeneration.

2008-10-01·Diabetic medicine : a journal of the British Diabetic Association2区 · 医学

Skin capillary permeability in the diabetic foot with critical limb ischaemia: the effects of a phVEGF165 gene product

2区 · 医学

Article

作者: Y. Kusumanto ; Andries Smit ; van der Johannes Hoeven ; de Karina Leeuw ; Nanno Mulder ; P. Oomen ; Geesiena Hospers

Abstract:

Aims To measure capillary permeability, assessed by skin capillary sodium fluorescein (NaF) leakage, in patients with diabetes mellitus with critical limb ischaemia (DM‐CLI) and to compare the effects of vascular endothelial growth factor (VEGF) with those of placebo.Methods NaF leakage was assessed in 17 patients with DM‐CLI, in 24 diabetes mellitus (DM) patients without clinical signs of macrovascular disease or neuropathy (DM‐C) and in 22 healthy control subjects. The 17 DM‐CLI patients were randomized to receive phVEGF165 gene product (n = 11) or placebo (n = 6). Measurements were repeated after 28 days.Results DM‐CLI patients had a longer dye arrival time (DAT), but NaF leakage was similar to control subjects, while capillary permeability was increased in DM‐C compared with control subjects. Leakage curve rose in patients receiving VEGF and fell in those receiving placebo, 28 days after administration. The decrease in DAT in the VEGF group was not significant, whilst DAT rose in the placebo group. Perfusion pressures were similar in the two groups.Conclusion No increase in capillary leakage in DM‐CLI was found, probably because an increased capillary filtration coefficient is counterbalanced by a marked fall in perfusion pressures. Increased capillary leakage may be one explanation for oedema formation after VEGF treatment.

2008-07-01·JACC. Cardiovascular imaging1区 · 医学

Radiolabeled Arginine-Glycine-Aspartic Acid Peptides to Image Angiogenesis in Swine Model of Hibernating Myocardium

1区 · 医学

Article

作者: Lorraine Schofield ; Athena Poppas ; Roland Haubner ; Tammy Donahay ; Lynne L. Johnson ; Mark Bouchard

OBJECTIVES:

Our aim was to image angiogenesis produced by endomyocardial injection of phVEGF165 in a swine model of hibernating myocardium using [123I]Gluco-arginine-glycine-aspartic acid (RGD) targeting the alphavbeta3 integrins.

BACKGROUND:

A noninvasive test to monitor the efficacy of therapy inducing angiogenesis is needed. The interaction between extracellular matrix and endothelial cells in sprouting capillaries is effected primarily by alphavbeta3 integrins that bind through RGD motifs.

METHODS:

At 21 +/- 4 days, after left circumflex coronary artery ameroid constrictor placement, 8 swine received endomyocardial injection of 1.2 mg phVEGF165 divided into 6 sites and 6 swine received saline (S) using nonfluoroscopic 3-dimensional endocardial mapping system (Noga)-guided delivery. After 20 +/- 6 days, 13 animals were injected with 6.4 +/- 1.7 mCi [123I]Gluco-RGD, 1 VEGF (vascular endothelial growth factor)-injected animal with I-123-labeled peptide control, and all animals with 2.5 +/- 0.4 mCi of Tl-201 and underwent single-photon emission computed tomography imaging. Blood flow and echocardiographic measurements were made at both time points and tissue analyzed for fibrosis and capillary density by lectin staining.

RESULTS:

Hibernating myocardium in the ameroid constrictor territory at time of injections was documented by reduced wall thickening compared with remote. Ratio of myocardial blood flow in left circumflex coronary artery/left anterior descending coronary artery territories increased by 15 +/- 11% in the VEGF animals and fell 13 +/- 12% in S-injected (p < 0.01). There was a small increase in wall thickening in constrictor territory after VEGF (8 +/- 17%) while in S-injected animals wall thickening fell by 23 +/- 31% (p = 0.01 vs. VEGF). Lectin staining as percent positive tissue staining for ameroid territory was higher in VEGF-injected compared with S-injected animals (2.5 +/- 1.5% vs. 0.87 +/- 0.52%, p = 0.01). Focal uptake of [123I]Gluco-RGD corresponding to Tl-201 defects was seen in VEGF-injected but not in S-injected animals. [123I]Gluco-RGD uptake in the ameroid territory as percent injected dose correlated with lectin staining (R2 = 0.80, p = 0.002).

CONCLUSIONS:

These data suggest that single-photon emission computed tomography imaging of radiolabeled RGD peptides may be a useful noninvasive method to monitor therapy that induces angiogenesis in the heart.

100 项与 VEGF-2 gene therapy(Steward St. Elizabeth's Medical Center of Boston, Inc.) 相关的药物交易

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适应症	最高研发状态	国家/地区	公司	日期
糖尿病性神经病变	临床2期	-	Steward St. Elizabeth's Medical Center of Boston, Inc.	-
糖尿病性神经病变	临床2期	-	Northwest University	-

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研究	分期	人群特征	评价人数	分组	结果	评价	发布日期


Phase III Comparison of Intramuscular Delivery of Naked Plasmid Encoding Vascular Endothelial Growth Factor with Placebo in Diabetic Patients with Critical Limb Ischaemia (ASGCT2002)	临床3期	慢性肢体威胁性缺血 diabetic retinopathy \| history of malignancy	-	Naked Plasmid Encoding Vascular Endothelial Growth Factor (phVEGF165)	鑰簾壓餘鏇簾鏇襯糧夢(遞觸選夢壓鑰獵顧壓構) = Complications were limited to transient lower extremity edema in three patients 鹹糧餘齋鬱製艱艱衊鑰 (鬱鏇夢鹽觸積築鏇觸襯 ) 更多	积极	2002-05-01