作者: Mannino, Deborah ; Esposito, Emanuela ; Forte, Stefano ; Colarossi, Lorenzo ; Casili, Giovanna ; Capra, Anna Paola ; Paterniti, Irene ; Basilotta, Rossella ; Lanza, Marika ; Giosa, Domenico ; Filippone, Alessia ; Sciacca, Dorotea

Anaplastic thyroid carcinoma (ATC) is the most aggressive type of thyroid cancer, characterized by rapid growth and invasion and poor prognosis. Due to its rarity and aggressive nature, ATC is a difficult condition to treat, thus knowledge of the mechanisms underlying its progression represents important research challenges. Benzyl isothiocyanate (BITC) is a natural compound that has shown promising anticancer properties. The aim of this study was to evaluate the antitumor effect of BITC in ATC, highlighting signaling pathways involved in BITC mechanism of action. This work included in vitro and in vivo studies. Results obtained indicate that BITC, both in vitro and in vivo, has the potential to slow the progression of ATC through interactions with autophagy, reduction in epithelial-mesenchymal transition (EMT) and attenuation of inflammation. In conclusion, this study identifies BITC as a compound worth further investigation for the development of new treatment strategies for this aggressive form of thyroid cancer.

2024-09-01·International Journal of Biological Macromolecules

An antibacterial film using κ-carrageenan loaded with benzyl isothiocyanate nanoemulsion: Characterization and application in beef preservation

Article

作者: Du, Xia-Xin ; Bi, Jing-Ran ; Hao, Hong-Shun ; Ge, Zi-Tong ; Hou, Hong-Man ; Zhang, Gong-Liang

Benzyl isothiocyanate (BITC) is a naturally active bacteriostatic substance and κ-carrageenan (KC) is a good film-forming substrate. In the present study, a nanoemulsion incorporating BITC was fabricated with a particle size of 224.1 nm and an encapsulation efficiency of 69.2 %. Subsequently, the acquired BITC nanoemulsion (BITC-NE) was incorporated into the KC-based film, and the light transmittance of the prepared composite films was lower than that of the pure KC film. Fourier transform infrared spectroscopy and scanning electron microscopy revealed that BITC-NE was compatible with the KC matrix. BITC-NE incorporation enhanced the tensile strength of the KC-based films by 33.7 %, decreased the elongation at break by 33.8 %, decreased the water vapor permeability by 60.1 %, increased the maximum thermal degradation temperature by 48.8 %, and decreased the oxygen permeability by 42 % (p < 0.05). Furthermore, the composite films showed enhanced antimicrobial activity against Staphylococcus aureus, Salmonella typhimurium, and Pseudomonas fluorescens. The developed KC-based composite films were applied to wrap raw beef, which significantly delayed the increase in total viable count, total volatile base nitrogen content, and thiobarbituric acid reactive substances, and prolonged the shelf-life of the raw beef by up to 10 days. These results indicated that the composite films prepared by incorporating BITC nanoemulsions into KC matrices have great antimicrobial application potential.

2024-09-01·Cancer Medicine

A new mechanism of cancer initiation that involves the transformation of hepatocytes into preneoplastic single hepatocytes and minifoci positive for glutathione S‐transferase P‐form (GST‐P) in rat livers: 3D analysis using a vibratome

Review

作者: Satoh, Kimihiko

AbstractBackgroundCancer initiation has long been “unknowable” in biology and medicine. In 1987, however, Moore and our research group observed single hepatocytes and minifoci that were strongly positive for glutathione S‐transferase P‐form (GST‐P) in the rat liver as early as 2 to 3 days after initiation by diethylnitrosamine prior to the induction of GST‐P+ foci and nodules. The induction of GST‐P+ single hepatocytes, precursors of GST‐P+ foci and nodules, was considered genetic. But, the details of the induction mechanism have remained unclear despite various examinations over a long period.MethodsMale Sprague‐Dawley rats (aged 6 weeks) were fed a basal diet containing either benzyl isothiocyanate (BITC, 0.5% by wt) or 2‐acetylaminofluorene (AAF, 0.04%) adlibitum for appropriate time intervals. All animals were anesthetized and euthanized. The livers obtained were excised, cut into 3‐ to 4‐mm‐thick slices and fixed in cold acetone at 4 °C. The liver specimens were then sliced into 25‐µm‐thick sections in PBS using an automated microtome (Vibratome 1500 Sectioning System, Vibratome Products, NY, USA). Immunocytochemical staining was performed in free solution, and the results were examined via digital light microscopy (Coolscope, Nikon, Tokyo).Results3D analysis using a vibratome showed that GST‐P is rapidly excreted into the bile of the liver of animals in response to strong carcinogenic stress caused by promoters or initiators. “Rapid biliary excretion of GST‐P” was widely and commonly observed in all hepatocytes, GST‐P+ single hepatocytes, minifoci, foci and nodules under appropriate conditions. Surprisingly, on the basis of these key findings, a new mechanism of cancer initiation involving the transformation of hepatocytes into GST‐P+ single hepatocytes and minifoci in animal livers was identified. In addition, the initiation process was determined to be nongenetic because mutation is an invisible rare event.ConclusionsThis short review describes several details about breakthrough findings on cancer initiation in rat livers, the application of 3D analysis to other cancers and the importance in the genetic analysis in malignant diseases.

100 项与 Benzyl isothiocyanate 相关的药物交易

登录后查看更多信息