Background:Fungi found in unique and competitive environments are abundant in bioactive compounds.Objective:The objective of this study is to isolate and identify secondary metabolites from fungi of unique ecological niches and evaluate their cytotoxicity.Methods:The compounds were isolated and purified using silica gel column chromatography, Sephadex LH-20 gel chromatography, and semi-preparative high-performance liquid chromatography (HPLC). The structures of the isolated compounds were determined using NMR and MS. The cytotoxic activities of these compounds were tested by the MTS assay.Results:Three diphenyl ethers, dechlorodihydromaldoxin (1), violaceol-I (2) and violaceol-II (3), one quinolinone compound, 2-(2-heptenyl)-3-methyl-4(1H)-quinolinone (4), and one α-pyrone nafuredin (5) were isolated from the fermented extracts of Pseudogymnoascus roseus S161. Compound 1 showed modest cytotoxicity against two human tumor cell lines A549 and MCF-7, with IC50 values of 87.12 and 51.07 µM, respectively.Conclusion:Five compounds were isolated from the fungus P. roseus S161. Compound 1 showed moderate cytotoxicity. This study provided a basis for the development of antitumor drugs.

2024-06-01·Fitoterapia

Colletodiol derivatives of the endophytic fungus Trichocladium sp.

Article

作者: Mándi, Attila ; Simons, Viktor E ; van Geelen, Lasse ; Gebhard, Christina ; Frank, Marian ; Coort, Annika ; Kurtán, Tibor ; Tran-Cong, Nam ; Kalscheuer, Rainer ; Albrecht, Dorothea

The OSMAC (one strain many compounds) concept is a cultivation-based approach to increase the diversity of secondary metabolites in microorganisms. In this study, we applied the OSMAC-approach to the endophytic fungus Trichocladium sp. by supplementation of the cultivation medium with 2.5% phenylalanine. This experiment yielded five new compounds, trichocladiol (1), trichocladic acid (2), colletodiolic acid (3), colletolactone (4) and colletolic acid (5), together with five previously described ones (6-10). The structures were elucidated via comprehensive spectroscopic measurements, and the absolute configurations of compound 1 was elucidated by using TDDFT-ECD calculations. For formation of compounds 3-5, a pathway based on colletodiol biosynthesis is proposed. Compound 6 exhibited strong antibacterial activity against methicillin-resistant Staphylococcus aureus with a minimal inhibitory concentration (MIC) of 0.78 μM as well as a strong cytotoxic effect against the human monocytic cell line THP1 with an IC₅₀ of 0.7 μM. Compound 8 showed moderate antibacterial activity against Mycobacterium tuberculosis with a MIC of 25 μM and a weak cytotoxic effect against THP1 cells with an IC₅₀ of 42 μM.

2019-09-01·Tetrahedron

Caryophyllene sesquiterpenes, chromones and 10-membered macrolides from the marine-derived fungus Pseudopestalotiopsis sp. PSU-AMF45

作者: Sarinya Hadsadee ; Haryadi N. Putra ; Siriporn Jungsuttiwong ; Vatcharin Rukachaisirikul ; Jariya Sakayaroj ; Souwalak Phongpaichit ; Sita Preedanon ; Saowanit Saithong

Three new caryophyllene sesquiterpenes, pestalotiopsins I-K, 1 new chromone, pseudopestalone, and 1 new 10-membered macrolide, decarestrictine Q, were isolated from the fermentation broth of the marine-derived fungus Pseudopestalotiopsis sp. PSU-AMF45 along with 12 known compoundsTheir structures were identified by spectroscopic data including 1D and 2D NMR.The absolute configuration of pestalotiopsin I was assigned based on single-crystal X-ray diffraction crystallog. whereas that of the secondary alc. moiety in pestalotiopsin J was established by Mosher's method.For pseudopestalone, the absolute configuration was determined by ECD spectrum associated with TD-DFT calculationThe antimicrobial activity of the isolated compounds was evaluated.

100 项与 RES 1214-1 相关的药物交易

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