Dual functional antibacterial nanofiber membranes: Polyhexamethylene biguanide-integrated alginate-chitosan-dye modified polyamide 56 for single-use biomedical applications

Article

作者: Chiu, Chen-Yaw ; Chen, Kuei-Hsiang ; Le, Quang-Vinh ; Wang, Chi-Yun ; Prapainainar, Paweena ; Liao, Ting-Cjia ; Liu, Bing-Lan ; Hanh, Nguyen The Duc ; Srinophakun, Penjit ; Ng, I-Son ; Chang, Yu-Kaung

Polyamide 56 (PA56) nanofiber membranes were functionalized with alginate (AG), chitosan (CS), reactive dyes (RG19, RR141), and poly(hexamethylene biguanide) (PHMB) to develop multifunctional antimicrobial membranes for single-use applications. The resulting membranes, PA56-AG-CS-RG19-PHMB and PA56-AG-CS-RR141-PHMB, achieved high antibacterial efficiencies of 97.12 % and 90.65 %, respectively, against Escherichia coli, demonstrating potent bacterial inhibition. The antimicrobial performance results from a synergistic dual mechanism. Chitosan disrupts bacterial adhesion and biofilm formation through electrostatic interactions, while PHMB compromises membrane integrity and interferes with intracellular processes. This combined action enhances bactericidal efficacy. The functionalization strategy also maintained excellent biocompatibility, with minimal cytotoxicity observed in L929 fibroblasts. Optimized concentrations of AG and CS were systematically evaluated to ensure balanced antibacterial performance and mechanical stability. These membranes, designed for single-use, exhibited significantly reduced antibacterial activity upon reuse, supporting their intended application. Overall, the integration of chemical and physical antimicrobial strategies within a nanofiber matrix presents a novel and effective approach. This strategy enables the development of next-generation materials suitable for real-world single-use biomedical applications, including wound dressings, food packaging, and protective textiles.

2018-07-03·Journal of toxicology and environmental health. Part A3区 · 医学

In vitrocharacterization of cutaneous immunotoxicity of immortalized human keratinocytes (HaCaT) exposed to reactive and disperse textile dyes

3区 · 医学

Article

作者: Gonçalves, Jenifer Pendiuk ; Grummt, Tamara ; Potrich, Francine Bittencourt ; Oliveira, Carolina Camargo de ; Winnischofer, Sheila Maria Brochado ; Trindade, Edvaldo da Silva ; Sehr, Andrea ; de Oliveira, Danielle Palma ; Leme, Daniela Morais ; Jacomasso, Thiago

Several synthetic dyes are used by textile industry for supplying the market of colored clothes. However, these chemicals have been associated with a variety of adverse human health effects, including textile dermatitis. Thus, there is a growing concern to identify textile dyes potentially as skin immunotoxicants. The aim of this in vitro study was to characterize the immunotoxic potential of reactive (Reactive Green 19 [RG19], Reactive Blue 2 [RB2], Reactive Black 5 [RB5]) and disperse (Disperse Red 1 [DR1]) textile dyes using a dermal cell line. For this purpose, a cell-based approach was conducted with immortalized human keratinocytes (KC) (HaCaT) using selected biomarkers of cutaneous inflammation including modulation of matrix metalloproteinases (MMP), oxidative stress such as reactive oxygen species (ROS) generation, and inflammatory cytokine profile. DR1 was the only dye able to trigger an immune response such as release of IL-12 cytokine, a potent co-stimulator of T helper 1 cell, which may be considered as a skin immunotoxicant. The reactive dyes including RB5 that were previously reported as skin sensitizers failed to induce inflammatory reactions under the conditions tested. The reactive dyes studied may pose a risk to human KC by induction of effects related to modulation of MMP-2 (RB5) and -9 (RB5 and RB2) and generation of ROS (RG19 and RB2). Thus, all these dyes need to be used with caution to avoid undesirable effects to consumers who may be exposed dermally.

2017-09-01·Journal of environmental management2区 · 环境科学与生态学

Effect of chelating agents on Reactive Green 19 decolorization through Fe0-activated persulfate oxidation process

2区 · 环境科学与生态学

Article

作者: Zhang, Sida ; Ding, Feng ; Zhao, Tongjun ; Chen, Hong ; Liu, Na

The effect of chelating agents on the decolorization of Reactive Green 19 (RG19) through Fe⁰ activated persulfate (PS/Fe⁰) process was investigated. Though other previous studies reported that chelating agent can enhance the degradation of organic contaminant in Fenton-like system, our finding showed that the presence of chelating agents would chelate free Fe²⁺ and minimize free Fe²⁺ concentration, which resulted in the retardation of RG19 decolorization. RG19 decolorization decreased to 7%, 21%, and 15% in the presence of sodium citrate, sodium EDTA, and sodium oxalate, compared with control test (without chelating agent, 99%) within 10 min. The degradation efficiencies decreased with increasing chelating agent concentrations because of complex formation with Fe²⁺. Higher PS concentration, Fe⁰ dosage, and temperature had an obvious enhancement for RG19 decolorization efficiency. Elucidation of RG19 containing sodium EDTA degradation pathways indicated that PS/Fe⁰ process could degrade RG19 to carboxylic acids and inorganic salts efficiently. The presence of sodium EDTA had no influence on by-products, and EDTA just played a chelating agent function for chelating Fe ions.

100 项与 RG-019 相关的药物交易

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