Five human neuroblastoma cell lines were examined for expression of a human melanosome-associated antigen (HMSA). Only cell line SK-N-SH reacted with a monoclonal antibody, HMSA-2, shown to recognize melanosomal glycoproteins. To further characterize the melanocytic lineages of SK-N-SH, three morphologically distinct clones designated SK-N-SH-N (neuroblast type), SK-N-SH-F (fibroblast type), and SK-N-SH-EP (epithelial type) were established by colony formation cloning. By fluorescence-activated cell sorter analysis and tyrosinase assay, we found that only SK-N-SH-EP and SK-N-SH-F reacted with HMSA-2 and had tyrosinase activity. These results suggest that epithelial-type and fibroblast-type cells appear to possess the melanocytic potential, but not neuroblast-type cells. Furthermore, SK-N-SH-EP was found to spontaneously convert to neuroblast-type or fibroblast-type cells, whereas SK-N-SH-N and SK-N-SH-F clones have remained morphologically stable. Our results suggest that at least one neuroblastoma cell line, SK-N-SH, may be an excellent model for investigating clonal maturation and the melanocytic differentiation of neuroblastoma.

1987-12-01·Talanta2区 · 化学

Analysis of the CCRMP Oka-2 rare-earth reference mineral of the britholite—apatite series by electrothermal atomic-absorption and inductively-coupled plasma atomic-emission spectrometry

2区 · 化学

Article

作者: Gupta, J G

The lanthanides and yttrium in the Canadian Certified Reference Materials Project (CCRMP) new rare-earth mineral reference material, Oka-2, were determined by electrothermal atomic-absorption and inductively-coupled plasma atomic-emission spectrometry (ICPAES) after sample decomposition with acids and separation of the rare-earth metals from phosphate and other matrix elements by precipitation as fluorides and oxalates. Thorium, yttrium and the common major and minor elements were determined by ICPAES after sample decomposition by fusion with lithium meta- and tetraborates and dissolution of the melt in a mixture of dilute nitric acid and ethylenediaminetetra-acetic acid solution. For comparison purposes, silicon, phosphorus, calcium, magnesium and iron were also determined by other methods. The results obtained are compared with other CCRMP values and with those obtained previously for a similar rare-earth mineral from the same geographical area. Oka-2 is considered to be a thorian intermediate member of the britholite-apatite series.

1982-03-01·Transfusion3区 · 医学

Red blood cell concentrates stored at 4 degrees C for 34 days in CPDA- 1, CPDA-2, or CPDA-3 anticoagulant-preservative, biochemically modified, and frozen and stored in the polyvinyl chloride plastic primary collection bag with 40% W/V glycerol at -80 degrees C

3区 · 医学

Article

作者: Melaragno, A. ; Gray, A. ; Valeri, C. R. ; Dennis, R. C. ; Valeri, D. A. ; Emerson, C. P.

Human red blood cell concentrates drawn from normal healthy volunteers with hematocrits of 85 volume% were stored in CPDA-1, CPDA-2, or CPDA-3 (citrate-phosphate-dextrose-adenine) anticoagulant at 4° for 35 days.After liquid storage the red blood cell concentrates were biochem. treated with a solution containing 100 mM pyruvate, 100 mM inosine, 100 mM phosphate, and 5 mM adenine to increase their 2,3-DPG and ATP levels which had deteriorated during storage at 4°.The rejuvenated red blood cells were frozen with 40% (weight/volume) glycerol in the poly(vinyl chloride) plastic primary collection bag and were stored at -80° for ≤3 mo.After thawing, the red blood cells were washed and stored at 4° for 24 h prior to autologous transfusion.The CPDA-1-stored red blood cells had a 24-h posttransfusion survival of 82%, and the index of therapeutic effectiveness (ITE) was 75%.The CPDA-2-stored red blood cells had a survival of 85%, with an ITE of 79%.The survival of the CPDA-3-stored red blood cells was 90%, and the ITE was 84%.All units had improved O delivery capacity, with 2,3-DPG and ATP levels of ∼150% of normal after thawing and storage at 4° for 24 h.

100 项与 Apta-2 相关的药物交易

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