Five human neuroblastoma cell lines were examined for expression of a human melanosome-associated antigen (HMSA). Only cell line SK-N-SH reacted with a monoclonal antibody, HMSA-2, shown to recognize melanosomal glycoproteins. To further characterize the melanocytic lineages of SK-N-SH, three morphologically distinct clones designated SK-N-SH-N (neuroblast type), SK-N-SH-F (fibroblast type), and SK-N-SH-EP (epithelial type) were established by colony formation cloning. By fluorescence-activated cell sorter analysis and tyrosinase assay, we found that only SK-N-SH-EP and SK-N-SH-F reacted with HMSA-2 and had tyrosinase activity. These results suggest that epithelial-type and fibroblast-type cells appear to possess the melanocytic potential, but not neuroblast-type cells. Furthermore, SK-N-SH-EP was found to spontaneously convert to neuroblast-type or fibroblast-type cells, whereas SK-N-SH-N and SK-N-SH-F clones have remained morphologically stable. Our results suggest that at least one neuroblastoma cell line, SK-N-SH, may be an excellent model for investigating clonal maturation and the melanocytic differentiation of neuroblastoma.

1988-09-01·Cancer1区 · 医学

Specification and use of a mouse monoclonal antibody raised against melanosomes for the histopathologic diagnosis of amelanotic malignant melanoma

1区 · 医学

Article

作者: Kazuo Maeda ; Kowichi Jimbow ; Kaori Maeda

The positive reactivity and specificity of a mouse monoclonal antibody (MoAb) human melanosome-specific antigen (HMSA) 2 raised against the melanosomal protein with amelanotic malignant melanoma on routine paraffin sections is reported. MoAb HMSA-2 identified cytoplasmic antigen with the following specifications: (1) a sharp heterogeneous expression in melanoma cells (acral lentiginous melanoma [ALM], 11 of 14; superficial spreading melanoma [SSM], 13 of 14; nodular melanoma [NM], one of three; and lentigo maligna melanoma [LMM], zero of two), whereas a diffuse homogeneous expression in cells of benign pigmented melanocytic nevi; and (2) an intense expression on amelanotic melanoma cells as opposed to a weak or negative expression on highly melanotic cells. The positive reactivity of MoAb HMSA-2 with amelanotic melanomas was exemplified by two shave-biopsy specimens of amelanotic subungual and plantar lesions, and by two cases of axillary and cervical amelanotic nodes that were left undiagnosed on routine hematoxylin and eosin (H & E) sections because of lack of melanin pigments. These were found, after diagnosis with MoAb HMSA-2, to possess the regressed primary lesions (both ALM).

1987-12-01·Journal of Investigative Dermatology1区 · 医学

Development of MoAb HMSA-3 and HMSA-4 Against Human Melanoma Melanosomes and Their Reactivities on Formalin-Fixed Melanoma Tissues

1区 · 医学

Article

作者: Kaori Yamana ; Kowichi Jimbow ; Kazuo Maeda

To elucidate the nature of melanosomal protein in normal and malignant melanocytes, two mouse monoclonal antibodies (MoAbs), designated MoAb HMSA-3 and MoAb HMSA-4, were developed by the solubilized melanosomes of human malignant melanoma. The specificity of the two MoAbs was characterized immunohistochemically by comparison with that of MoAb HMSA-2 in various forms of melanoma tissues. MoAb HMSA-3 and MoAb HMSA-4 were IgM, k subclass while MoAb HMSA-2 was IgG1, k subclass. The three MoAbs possessed many similarities; (a) positive reactivity in formalin-fixed and paraffin-processed specimens, (b) identification of cytoplasmic antigen(s) in melanoma cells, (c) negative reactivity with normal epidermal melanocytes on paraffin-sections, and (d) intense reaction with amelanotic melanoma cells, particularly in superficial spreading and acral lentiginous melanoma and in metastatic lymph nodes. The three MoAbs, however, identified the different cells on the same serial sections, suggesting that the three may recognize the different epitopes. Thus in 32 cases of primary and metastatic melanomas examined, one of the three MoAbs always showed a positive reactivity, though the other two were negatively or weakly reacted. Our study indicated that the melanosomal protein may provide a unique source to develop MoAbs which identify malignant melanocytes on routine paraffin sections.

100 项与 Apta-2 相关的药物交易

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