Developing an accurate, sensitive and visual strategy for quickly identifying biomarkers α-ketoglutaric acid (α-KA) is crucial for early disease diagnosis. Herein, we introduce a new fluorescence material, Eu³⁺@COMOC-4, synthesized by post-synthesis modification of a lanthanide-functionalized metal-organic frameworks (MOFs). This material serves as a visual fluorescence sensor to detect α-KA, with a distinct fluorescence response. Particularly, the sensor exhibits many advantages, such as good selectivity, high sensitivity and quick response. The quenching mechanism between the sensor and α-KA was also illustrated in detail. More significantly, a smartphone-integrated test paper fluorescence sensing platform was further developed for α-KA detection, which enables sensitive, real-time, and visual detection of α-KA. This work provides a sensitive and real-time biomarker detection strategy, which is expected to expand the application of MOFs in significant fields.

2025-01-01·Food Chemistry

Natural α-glucosidase inhibitors from Aquilaria sinensis leaf-tea: Targeted bio-affinity screening, identification, and inhibition mechanism

Article

作者: Fei, Tao ; Lin, Xue ; Liu, Yingxin ; Li, Fangliang ; Wang, Lu ; Liu, Zhonghua

Aquilaria sinensis leaves have long been consumed as a popular replacement tea for lowering postprandial blood glucose levels, but their specific functional components remain unclear. In this study, Aquilaria sinensis leaf-tea 70 % ethanol extract (ALTE) exhibited excellent anti-α-glucosidase activity (IC₅₀ = 6.93 ± 1.91 μg/mL) and promoted glucose consumption ability in 3 T3-L1 preadipocyte cells. Subsequently phenolic compositions of ALTE were identified for the first time. After that, five potential α-glucosidase inhibitors (α-GIs) including cynaroside-3,5-diglucose, malvidin 3-glucose, epicatechin, epigallocatechin gallate, and dihydromyricetin in ALTE were screened using a targeted bio-affinity ultrafiltration-HPLC/MS method. Moreover, these five α-GIs all showed good anti-α-glucosidase effects and glucose consumption-promoting ability. Furthermore, the binding properties and inhibition mechanisms of five α-GIs to α-glucosidase were further analyzed via enzyme inhibition kinetics, molecular docking, and molecular dynamics simulation. This study confirms that Aquilaria sinensis leaf-tea is effective in preventing post-hyperglycemia in vitro models, suggesting potential for future research in human trials.

2024-12-01·Meat Science

Effects of feeding patterns on production performance, lipo-nutritional quality and gut microbiota of Sunit sheep

Article

作者: Duan, Yan ; Zhang, Taiwu ; Jin, Ye ; Xie, Junkang ; Zhai, Maoqin ; Kang, Letian ; Yang, Le ; Wang, Weihao ; Zhao, Xin ; Liu, Ting

This study aimed to investigate the impact of feeding patterns on the production performance, lipo-nutritional quality, and gut microbiota of Sunit sheep. A total of 24 sheep were assigned to two groups: confinement feeding (CF) and pasture feeding (PF) groups. After 90 days, the CF group exhibited significantly increased average daily gain, carcass weight, backfat thickness, and intramuscular fat content of the sheep, whereas the PF group showed significantly increased pH_24h and decreased L^∗ value and cooking loss of the longissimus lumborum (LL) muscle (P < 0.05). In the PF group, the contents of linoleic, α-linolenic, and docosahexaenoic acids were considerably higher and the n-6/n-3 polyunsaturated fatty acid ratio was significantly lower (P < 0.05). Furthermore, the triglyceride, cholesterol, and nonesterified fatty acid levels in the serum of the CF group significantly increased, whereas the enzyme contents of fatty acid synthase (FASN) and hormone-sensitive lipase (HSL) in the LL muscle of the PF group were markedly elevated (P < 0.05). The PF group also showed altered expression of lipid metabolism-related genes, including upregulated FASN, HSL, fatty acid binding protein 4 (FABP4), and peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) (P < 0.05). Meanwhile, differences were observed in the abundance of key bacteria and microbiota functions between the groups. Correlation analysis revealed that production performance and lipid metabolism may be related to the differential effects of bacteria. In conclusion, the transition in the feeding patterns of Sunit sheep caused changes in the gut microbial community and lipid metabolism level in the muscle as well as differences in fat deposition and meat quality.

100 项与 α-Glucosidase(Pharming Technologies BV) 相关的药物交易

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