Hinokiflavone

Abstract: Some active substances in Phyllanthus emblica not only endow Phyllanthus emblica with specific biol. functions but also provide its astringency.It is not a good choice to directly remove these active substances to improve the astringency.In order to clarify the astringent active substances in Phyllanthus emblica and their interaction mechanism with oral protein, the composition and structure of the active substances in Phyllanthus emblica were identified by UHPLC-Q-exactive-orbitrap-MS technol., and the main astringent substances were identified.Combining turbidity and electronic tongue measurement technol. by tracking the morphol. changes of the main astringent substances and the products of protein, a method for studying the interaction between the astringent substances of Phyllanthus emblica and protein in vitro was established.Further, the interaction mechanism between the astringent substance of Phyllanthus emblica and protein was analyzed at the mol. level by fluorescence scanning and CD.The results showed that 29 active substances were identified from Phyllanthus emblica by accurate quality measurement and comparison, and ellagic acid was identified as the main astringent active component in Phyllanthus emblica.On this basis, according to the relationship between the astringent taste of ellagic acid and the turbidity of ellagic acid/amylase mixture, a method for in vitro study of Phyllanthus emblica was successfully developed (R² = 0.9954), which provided a premise for in vitro study on the formation and determination of astringent taste of Phyllanthus emblica.Fluorescence quenching and CD anal. showed that ellagic acid induced the conformational change of protein mol. and revealed that the interaction between ellagic acid and protein of Phyllanthus emblica was the main cause of astringency.The results provide inspiration for exploring the astringent components of Phyllanthus emblica and the formation mechanism of astringency.

Ferroptosis, a cell death regulation process dependent on iron levels, represents a promising therapeutic target in cancer treatment. However, the scarcity of potent ferroptosis inducers hinders advancement in this area. This study addresses this gap by screening the PubChem database for compounds with favorable ADMET properties to identify potential GPX4 inhibitors. A structure-based virtual screening was conducted to compare binding affinities of selected compounds to that of RSL3. The candidates-isochondrodendrine, hinokiflavone, irinotecan, and ginkgetin-were further analyzed through molecular dynamics (MD) simulations to assess their stability within the GPX4-ligand complexes. The computed binding free energies for RSL3, isochondrodendrine, hinokiflavone, irinotecan and ginkgetin were -80.12, -107.31, -132.03, and -137.52 and -91.11 kJ/mol, respectively, indicating their significantly higher inhibitory effects compared to RSL3. These findings highlight the potential for developing novel GPX4 inhibitors to promote ferroptosis, warranting further experimental validation.