Homocysteine, inflammatory factors, safety study of scutellarin for injection in patients with acute cerebral infarction - a single center, randomized, open label clinical trial

开始日期2021-07-19

申办/合作机构

云南省第一人民医院

100 项与 Scutellarin 相关的临床结果

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100 项与 Scutellarin 相关的转化医学

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100 项与 Scutellarin 相关的专利（医药）

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1,894

项与 Scutellarin 相关的文献（医药）

2025-06-01·Phytomedicine

Scutellaria baicalensis Extracts Restrict Intestinal Epithelial Cell Ferroptosis by Regulating Lipid Peroxidation and GPX4/ACSL4 in Colitis

Article

作者: Kang, An ; Zhang, Jingyan ; Fang, Dan ; Tan, Bingyan ; Wu, Hong ; Han, Tai ; Hu, Bing ; Cai, Hong

BACKGROUNDFerroptosis in colonic epithelial cells has been implicated in the development of ulcerative colitis (UC) and the accompanying gut leakage. Scutellaria baicalensis Georgi (Scu) is widely used herb medicine for alleviating UC.PURPOSEWe aimed to clarify the therapeutic effect of Scu on UC by inhibiting intestinal epithelial cell ferroptosis and explore its regulatory mechanisms on lipid peroxidation and the GPX4/ACSL4 pathways.METHODSUPLC-Q-TOF/MS was employed to analyze chemicals in the herbal extract and the colonic exposure of prototypes in Scu-treated mice. Additionally, the main compounds were quantified using HPLC-UV. The ameliorative effects of Scu were comprehensively explored in a UC mouse model established by feeding with dextran sulfate sodium (DSS). HPLC-MS based metabolomic studies were conducted to identify the differential metabolites in colon tissues from Scu or vehicle treated UC mice. Network pharmacology was conducted for target prediction and potential pathway analysis. In conjunction with these bioinformatic analyses, we performed RT-qPCR, immunofluorescence, immunohistochemistry and immunoblotting to elucidate the regulatory mechanisms of Scu on ferroptosis-related pathways in both in vivo and in vitro models.RESULTS78 chemical constituents in Scu were characterized, with 42 detected in the colonic tissues of Scu-treated mice. Scu could alleviate UC related symptoms in mice, including increased colon length and decreased pathological score. Furthermore, Scu inhibited pro-inflammatory cytokines and mediators, while improving gut barrier function by increasing the expression of ZO-1 and Occludin at both mRNA and protein levels. Based on metabolomic studies, a total of 71 differential metabolites exhibited a reversal trend following Scu administration. These findings, combined with results from network pharmacology, suggest that arachidonic acid (AA) metabolism and ferroptosis may serve as potential pathways for Scu intervention in UC. Further experiments indicated that the amelioratory actions of Scu on ferroptosis partially contributed to its modulation on lipid peroxidation and its regulatory influence on the GPX4/ASCL4 axis to ameliorate UC. When AA was administered at the same time as concurrently with Scu, the regulatory effects of Scu on ferroptosis, GPX4/ASCL4 axis, and its protective effects against UC were significantly reduced. Moreover, the inhibitory effect of Scu on ferroptosis was weakened when we knocked down GPX4 or overexpressed ACSL4 in vitro.CONCLUSIONThe ameliorative effect of Scu in UC is closely related to the regulation of lipid peroxidation and GPX4/ASCL4 mediated intestinal epithelial ferroptosis.

2025-04-22·Current Pharmaceutical Design

Unraveling the Therapeutic Potential of Scutellarin for Clear Cell Renal Cell Carcinoma: A Comprehensive Molecular Analysis

Article

作者: Bai, Yangyang ; Guo, Yilin ; Chen, Ruiting ; Sun, Jijian ; Liu, Ranlu

Background:Clear cell renal cell carcinoma (ccRCC), the most common subtype of renal cell carcinoma, is a significant global health issue. Despite advancements in surgery and systemic therapies, drug resistance remains a challenge, and more effective treatments are needed. Scutellarin, a natural flavonoid with anticancer properties, is a promising therapeutic option for ccRCC.Methods:This present study identified the potential target genes of scutellarin by searching four databases and utilized the TCGA-KIRC and GSE53757 datasets to identify ccRCC features genes. Protein-protein interaction networks and molecular complex detection analyses determined the hub genes through which scutellarin acts on ccRCC. Differential expression, receiver operating characteristic analysis, survival, and immune cell infiltration analyses were conducted successively on these hub genes in tumor and normal tissues to verify their clinical significance. The intracellular mechanism of the hub genes was explored using a single-cell dataset (GSE222703) to elucidate the intracellular pathway through which scutellarin exerts its anti-ccRCC effects. At last, molecular docking and molecular dynamics simulations were performed to confirm the stability of the receptor protein of the hub gene binding to scutellarin.Results:158 scutellarin targets were collected and identified through database searches. Analyzing the TCGA-KIRC and GSE53757 data separately identified finally 132 ccRCC feature genes through differential expression analysis and WGCNA. Protein-protein interaction network and molecular complex detection analyses revealed 26 hub genes potentially involved in hinge pathways of scutellarin in ccRCC. Differential expression analysis revealed significant differences in the expression of these hub genes between tumor and normal tissues. Receiver operating characteristic analysis demonstrated the fine diagnostic efficacy of these hub genes. Survival analysis indicated that the hub genes TYMS and CDCA2 were associated with a better prognosis, whereas the remaining hub genes had a poorer prognosis. Enrichment analysis revealed that hub genes mainly involved oxidative stress and cell cycle regulation. Single-cell RNA sequencing analysis suggested that most hub genes exert their effects on T helper cells. Molecular docking results showed stable docking of hub genes with scutellari, except for SPAG5 and ASPM. Molecular dynamics simulations of the most stable docking sites, KIF20A, TYMS, and KIF18B, indicated stable complex formation compared with that of the internal reference protein GAPDH.Conclusion:This integrated study provides a comprehensive analysis of the molecular targets and pathways affected by scutellarin in ccRCC. The identified hub genes and their related pathways present exciting prospects for therapeutic intervention and highlight the potential of scutellarin as a novel treatment for ccRCC. Additional research is necessary to investigate the precise molecular mechanisms and therapeutic advantages of scutellarin in preclinical and clinical contexts.

2025-04-01·Phytochemical Analysis

Dual Assay Validation of Rosmarinus officinalis Extract as an Inhibitor of SARS‐CoV‐2 Spike Protein: Combining Pseudovirus Testing, Yeast Two‐Hybrid, and UPLC‐Q Exactive Orbitrap‐MS Profiling

Article

作者: Tian, Chenjing ; Duan, Siyan ; Zu, Duntao ; Yang, Jianni ; Zhou, Haibo ; He, Qinghui ; Li, Shengrong ; Luo, Rufeng ; Yan, Shunxin ; Huang, Dingqiang ; Chen, Wenlin ; Yuan, Yujia ; Huang, Yujing ; Zheng, Junxia ; Lin, Fulong

ABSTRACTIntroductionThis study evaluates the effectiveness of Traditional Chinese Medicine (TCM) extracts in blocking the interaction between the SARS‐CoV‐2 Spike protein and human ACE2 receptor, utilizing a dual‐method approach to explore the antiviral potential of natural compounds.ObjectivesThis work aims to evaluate the capability of TCM extracts in inhibiting the SARS‐CoV‐2 Spike protein and ACE2 receptor interaction using advanced biochemical assays.MethodsA dual‐method screening approach was utilized, beginning with a pseudovirus assay to assess the inhibition capabilities of TCM extracts in vitro, followed by a split‐ubiquitin yeast two‐hybrid (Y2H) system to validate interactions in live cells. Active compounds were characterized and quantified using UPLC‐Q‐Exactive‐Orbitrap‐MS.ResultsAmong the 91 TCM extracts tested, Rosmarinus officinalis exhibited the most potent inhibition in both pseudovirus and Y2H assays, significantly reducing viral entry and disrupting the Spike–ACE2 interaction. Comprehensive chemical profiling via UPLC‐Q‐Exactive‐Orbitrap‐MS identified 132 compounds, including phenolics, flavonoids, and terpenoids.ConclusionThis research validates the use of TCM extracts in viral inhibition strategies, demonstrating the utility of integrating traditional remedies with modern scientific approaches to discover new therapeutic agents.

100 项与 Scutellarin 相关的药物交易

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