Previous research shows that gypenoside could keep blood lipid and blood sugar under control in an effective way. In this study, we intend to explore whether gypenosides have clinical improvement effects on metabolic diseases such as diabetes, hyperlipidemia, and non-alcoholic fatty liver and their cardiovascular protective potential. It is designed to recruit 150 patients with metabolic syndrome diagnosed who meet the criteria for enrollment, and randomly divide them into three groups. Gynostemma powder, with two different saponins content (12% and 8%) from Pingli County, Shaanxi Province, and spinach powder with almost no saponins were used to conduct the intervention on patients. Patients will be followed up regularly at the beginning of each month from the first enrollment until the third month. At each follow-up, data of patient's anthropometric indicators as well as clinical inspection indicators related to metabolism (blood routine, liver function, etc.) will be recorded. In addition, patient's blood, hair, urine, and stool samples will be collected to further explore the mechanism of diseases.

开始日期2022-03-01

申办/合作机构

西安交通大学第一附属医院

NCT02976766 / Terminated临床2期IIT

Gypenosides Treatment for Optic Neuritis: a Randomised, Double-blind, Placebo-controlled Trial

The purpose of this study is to determine whether gypenosides are neuroprotective in patients with acute optic neuritis.

开始日期2017-02-04

申办/合作机构

广西医科大学第一附属医院

100 项与绞股蓝提取物相关的临床结果

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100 项与绞股蓝提取物相关的转化医学

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100 项与绞股蓝提取物相关的专利（医药）

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184

项与绞股蓝提取物相关的文献（医药）

2025-01-01·JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

To explore the mechanism of gypenosides in the treatment of liver injury in rats based on GC-MS metabolomics and bile acid metabolism pathway

Review

作者: Chen, Yu ; Yue, Rong ; Zhang, Zhiru ; Wang, Miao ; Wang, Yibo ; Ma, Lizhou

Gynostemma pentaphyllum is a herbaceous vine of Cucurbitaceae family, and its principal pharmacological components, gypenosides (GPs), have been proved to be effective in various liver diseases. However, the mechanisms of GPs on liver injury are still to be studied for further. This investigation utilized the CCl₄-induced liver injury rat model (LI) to comprehensively explore the mechanism of action of GPs in the treatment of chemical liver injury by comparing the metabolomic changes in four groups rats. In this study, the therapeutic efficacy of GPs in a liver injury rat model induced by weekly gavage of CCl₄ was evaluated by inflammatory factors, oxidative damage indexes, and histopathological sections. Then, GC-MS technology was used to identify the metabolic profile of GPs in treating liver injury. Finally, the content variation of metabolites (BAs and SCFAs) was measured to elucidate the mechanism of GPs in the treatment of CCl₄-induced liver injury. After 8 weeks of administration, GPs effectively reduced the degree of LI and appeared a substantial tendency of reversing in the levels of MDA, GSH, CYP7E1, CYP7A1 and CYP27A1. Untargeted metabolomics suggested that GPs may play a role in BAs and SCFAs metabolism. Targeted metabolomics and ELISA confirmed the key role of GPs in increasing SCFAs levels and regulating BAs metabolism. Overall, this study indicated that GPs can alleviate CCl₄-induced liver injury. And GPs may exert beneficial effects on LI by affecting their metabolites (SCFAs and BAs).

2025-01-01·JOURNAL OF ETHNOPHARMACOLOGY

Gypenosides alleviate oxidative stress in the hippocampus, promote mitophagy, and mitigate depressive-like behaviors induced by CUMS via SIRT1

Article

作者: Sun, Jianfei ; Li, Yingfeng ; Wu, Ning ; Li, Jingqi ; Rao, Hui ; Liu, Yuhang ; Xiu, Jiangfan

ETHNOPHARMACOLOGICAL RELEVANCE:

The use and efficacy of Gynostemma [Gynostemma pentaphyllum (Thunb.) Makino], a versatile traditional Chinese herb, was first documented in the renowned pharmacopoeia, "Compendium of Materia Medica". Gypenosides (Gps), saponin components are the primary constituents responsible for its biological activities and clinical effects, which include antioxidant, immunoregulatory, antitumor, and neuroprotective properties. Pharmacological studies have shown that Gps has the potential to combat depression. However, the exact molecular mechanisms underlying its antidepressant effects remain unclear.

AIM OF THE STUDY:

This study aims to elucidate the mechanisms underlying the antidepressant effects of Gps through antioxidative stress, utilizing an integrated approach that includes network pharmacology, molecular simulations, and experimental validation.

MATERIALS AND METHODS:

Sprague-Dawley rats were subjected to chronic unpredictable mild stress (CUMS) and were orally administered doses of Gps (50 and 100 mg/kg) and fluoxetine (10 mg/kg). The regulatory effects of Gps on depression-like behaviors in CUMS rats and their impact on oxidative stress levels in the hippocampus region were evaluated. Network pharmacology was used to investigate the mechanisms by which Gps affects oxidative stress in depression, and was accompanied by molecular docking and dynamics simulations. CUMS rats were treated orally with Gps (100 mg/kg) and injected with EX527 for rescue experiments to validate the role of SIRT1 in antioxidative stress and evaluate the impact of Gps on mitophagy.

RESULTS:

Gps ameliorated depression-like behaviors induced by CUMS in rats. The improvements observed included an increased sucrose preference, reduced immobility time in the tail suspension and forced swim tests, and an increased movement distance in the open-field test. Additionally, Gps effectively reduced reactive oxygen species, malondialdehyde, and 8-hydroxy-2'-deoxyguanosine levels in the hippocampus, while increasing the contents of ATP, catalase, superoxide dismutase, and glutathione, indicating an increased capacity for antioxidative stress in the hippocampus. Furthermore, Gps increased the number of neuronal cells in the hippocampal CA1 region and the level of mitochondrial autophagy, with SIRT1 as a potential key target. Inhibition of SIRT1 expression by exposure to EX527 reversed the beneficial effects of Gps, further validating the critical role of SIRT1 in the regulation of oxidative stress and improving depression-like behavior.

CONCLUSION:

Gps improved the antioxidative stress capacity of the hippocampus and promoted mitophagy in CUMS rats through SIRT1, thus protecting hippocampal neurons and improving depression-like behavior.

2024-09-18·JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Gypenoside LXXV Alleviates Colitis by Reprograming Macrophage Polarization via the Glucocorticoid Receptor Pathway

Article

作者: Wu, Wenjing ; Wan, Mengqi ; Zhou, Yifa ; Cheng, Hairong ; Zhu, Xuepeng ; Qu, Xian ; Hu, Chenxing

An imbalance in the macrophage phenotype is closely related to various inflammatory diseases. Here, we discovered that gypenoside LXXV (GP-75), a type of saponin from Gynostemma pentaphyllum, can reprogram M1-like macrophages into M2-like ones. On a mechanistic level, GP-75 inhibits NF-κB-COX2 signaling by targeting the glucocorticoid receptor (GR). Administration of GP-75, either orally or by intraperitoneal injection, significantly alleviates ulcerative colitis in mice, a pathogenesis associated with macrophage polarization. Clodronate liposomes, which deplete macrophages in mice, as well as GR antagonist RU486, abrogate the anticolitis effect of GP-75, thus confirming the pivotal role of macrophages in GP-75 function. We also showed that GP-75 has no toxicity in mice. Overall, this is the first report that demonstrates the effect of GP-75 on macrophage reprograming and as an agent against colitis. Because G. pentaphyllum is gaining popularity as a functional food, our findings offer new perspectives on the use of gypenosides as potential nutraceuticals for medical purposes.

项与绞股蓝提取物相关的新闻（医药）

2024-08-11

·蒲公英Ouryao

【新疆】金箔、绞股蓝、驼峰油、乳鸽肉质量标准发布

来源：新疆药监局编辑：wangxinglai2004 近日，新疆自治区药监局发布了4份自治区地区性民间习用药材（中药饮片）质量标准，分别为金箔、绞股蓝、驼峰油、乳鸽肉，自发布之日起实施。根据《中华人民共和国药品管理法》《药品标准管理办法》新疆维吾尔自治区药品监督管理局组织制订了金箔、绞股蓝、驼峰油、乳鸽肉新疆维吾尔自治区地区性民间习用药材(中药饮片)标准，现予以发布，自发布之日起实施。金箔、绞股蓝、驼峰油、乳鸽肉的同一药材国家药品标准实施后，我局制定的地区性民间习用药材标准自行废止。 01标准号：2024YC-0009 金箔 Jinbo AURUMFOIL 本品为自然元素铜族矿物自然金经加工锤成的薄片，主要含自然金(Au)。将适量黄金放入多层叠好的纸层中，用木槌在上面上时间反复锤打，锤成薄片，即为金箔。【性状】本品呈正方形、长方形或不规则形薄片，大小不一。表面呈金黄色。具极强的金属光泽。不透明。体轻，较柔韧。无气味。【鉴别】取本品适量，置酒精灯上灼烧，皱缩，不成灰，不变色。【性味与归经】辛、苦，平。归心、肝经。【功能与主治】镇心，安神，解毒。治惊痫、癫狂，心悸，疮毒。【用法与用量】可入糖膏、蜜膏、片、丸散剂等，一般多作丸药挂衣。外用适量，研末撒患处。【注意事项】阳虚气陷，下痢清冷者忌服。【贮藏】置干燥处。 02标准号：2024YC-0010 绞股蓝 Jiaogulan HERBAGYNOSTEMMATISPENTAPHYLLI 本品为葫芦科植物绞股蓝Gynostemmapentaphyllum(Thunb.)Makino的干燥地上部分。夏、秋茎叶茂盛时，采割地上部分，除去杂质，水洗，干燥或切段、阴干。【性状】本品卷曲成团或成长段状。茎被短柔毛或近无毛，呈黄绿色或褐绿色，直径1-3mm，具细纵棱线，质韧，不易折断；卷须侧生于叶柄基部。叶互生，薄纸质或膜质，皱缩，易碎落，完整叶展开呈鸟足状复叶，边缘有锯齿。气微，微苦。【鉴别】（1）本品茎横切面：表皮细胞1列，扁平，外壁角质增厚，有时可见多细胞非腺毛；角隅处有厚角组织，常为4-6列细胞；皮层内有数个大小不等的维管束，断续排列成环；韧皮部外缘围绕有半月形纤维束；木质部导管圆形，多单个存在。髓部薄壁细胞中含淀粉粒。本品粉末灰绿色。腺毛头多由4个细胞组成，腺柄由1-2个细胞组成。非腺毛有多细胞和单细胞两种，多细胞非腺毛表面具角质线状纹理。气孔不定式，副卫细胞4-6个。具缘纹孔、螺纹或环纹导管。纤维多碎断，有的内含淀粉粒。淀粉粒椭圆形或不规则形，脐点点状、短缝状或人字形，层纹不明显。石细胞棕黄色，单个或成群散在，呈类方形、类长方形或纤维状，壁厚，层纹明显，孔沟清晰。（2）取本品粉末2g，加甲醇30ml，加热回流2小时，放冷，滤过，滤液蒸干，残渣加水20ml使溶解，用乙醚振摇提取2次，每次10ml，弃去乙醚液，水液用水饱和的正丁醇振摇提取3次，每次20ml，合并正丁醇提取液，蒸干，残渣加甲醇2ml使溶解，作为供试品溶液。另取绞股蓝对照药材2g，同法制成对照药材溶液。照薄层色谱法（《中国药典》2020年版四部通则0502）试验，吸取上述两种溶液各5μl，分别点于同一硅胶G薄层板上，以环己烷-丙酮（2:1）为展开剂，展开，取出，晾干，喷以10%硫酸乙醇溶液，在105℃加热至斑点显色清晰，分别置日光和紫外光灯（365nm）下检视。供试品色谱中，在与对照药材色谱相应的位置上，显相同颜色的斑点或荧光斑点。【检查】水分不得过13.0%（《中国药典》2020年版四部通则0832第二法）。总灰分不得过13.0%（《中国药典》2020年版四部通则2302）。酸不溶性灰分不得过2.0%（《中国药典》2020年版四部通则2302）。【浸出物】照水溶性浸出物测定法（《中国药典》2020年版四部通则2201）项下的冷浸法测定，不得少于14.0%。饮片【炮制】除去杂质，洗净，切段，干燥。【性状】本品为不规则的段，根茎、茎、叶、花混合。根茎细，圆柱形。茎细弱，具纵棱，黄绿色或黄白色，卷须生于叶腋，纤细，2歧或单一。叶片多皱缩或破碎，薄纸质或膜质，完整者通常由5-7小叶组成鸟趾状复叶，小叶片边缘具锯齿，黄绿色，两面疏被短毛，有极短的小叶柄。气微，味微苦、微甘。【鉴别】【检查】同药材。【浸出物】照水溶性浸出物测定法（《中国药典》2020年版四部通则2201）项下的冷浸法测定，不得少于13.0%。【性味与归经】苦、微甘、寒。归肺、脾、肾经。【功能与主治】清热解毒，止咳祛痰，益气养阴。用于胸膈痞闷，痰阻咳嗽，心悸气短，眩晕头痛，健忘耳鸣，自汗乏力。【用法与用量】6～10g。【贮藏】置通风干燥处。 03标准号：2024YC-0011 驼峰油 Tuofengyou ADEPSCAMELE تۆگەلوكىسىمېيى 本品为双峰驼CamelusbactrianusL.驼峰脂肪，经炼制，滤过，冷却后而成的脂肪油。【性状】本品呈均匀脂状，乳白色，触之松软细腻，有微弱的特殊膻味。本品不溶于水，易溶于热乙醇，在石油醚（60~90℃）能缓慢溶解。熔点应为37～43℃（《中国药典》2020年版四部通则0612熔点测定第二法）。【检查】脂肪与脂肪油测定照脂肪与脂肪油测定法（《中国药典》2020年版四部通则0713）测定。酸值应不大于5.0 皂化值160.0～242.0 碘值25.0～57.0 【炮制】将驼峰脂切块，文火熬制至油渣呈干瘪黄色，滤过即得。【性质】味甘，性温。【功能与主治】补虚润燥，祛风活血，消肿解毒。主治体虚劳乏，肌肤不仁，皮肤瘙痒，筋肉挛急，疮疡肿毒，痔漏。【用法与用量】外用适量涂敷。【贮藏】置阴凉干燥处。 04标准号：2024YC-0012 乳鸽肉 Rugerou MUSCULUSCOLUMBAE باچكاگۆشى 本品为鸠鸽科动物原鸽、家鸽和岩鸽的幼鸽除去头、爪、毛、内脏及肛门的新鲜全体。将30日龄内，重量300-500克的幼鸽屠宰，放血后除去头、爪、毛、内脏及肛门。切块鲜用或冷冻备用。【性状】本品为鲜品或冷冻品，呈不规则块状，皮肤淡黄色，具突起的毛孔；肌肉绛红色，具光泽，纤维状，肉质细腻；皮下脂肪少，白色至淡黄色。气微腥，无异味。【鉴别】（1）本品粉末（切块，混匀，绞碎）暗棕色至红棕色。肌纤维长条形，直径5~37µm，成束状或片状散在，淡黄色或近无色，有细密的波状纹理。脂肪油滴近无色，散在。（2）取本品，切块，混匀，绞碎，取约2g，加40%乙醇10ml，超声处理20分钟，滤过，取滤液作为供试品溶液。另取丙氨酸对照品，加40%乙醇制成每1ml含0.5mg的溶液，作为对照品溶液。照薄层色谱法（《中国药典》2020年版四部通则0502）试验，吸取供试品溶液4μl，对照品溶液1μl，分别点于同一硅胶G薄层板上，以正丁醇-冰醋酸-水（4:2:0.3）为展开剂，展开，取出，晾干，喷以茚三酮试液，在105℃加热至斑点显色清晰。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的斑点。【炮制】将30日龄内，重量300-500克的幼鸽屠宰，放血后除去头、爪、毛、内脏及肛门，切块鲜用或冷冻备用。【性质】二级干热，味咸。【功能与主治】生干生热，祛寒强筋，燥湿健肌，补虚强身，散气止痛。主治湿寒性或黏液质性疾病，如瘫痪，面瘫，颤抖症，全身虚弱，陈旧性头痛，关节痛，两胁痛等。【用法与用量】内服：根据病情食用或入制剂中。【贮藏】冷冻保存。

申请上市上市批准

2024-07-01

·蒲公英Ouryao

【新疆】绞股蓝、驼峰油、乳鸽肉药材标准公示

来源：新疆药监局编辑：wangxinglai2004 近日，新疆自治区药监局发布了3份地区性民间习用药材标准公示，涉及品种为绞股蓝、驼峰油、乳鸽肉，公示期同为1个月。 01 公示通知原文我局拟制定绞股蓝、驼峰油、乳鸽肉地区性民间习用药材质量标准，为确保标准的科学性、合理性和适用性，现公示征求社会各界意见(详见附件),公示期为一个月。请相关单位认真研核，若有异议，请及时来函提交反馈意见，并附相关说明、实验数据和联系方式，来函需加盖公章，收文单位为“自治区药品监督管理局”同时将公函扫描件电子版发送至指定邮箱，公示期满未回复意见即视为对公示标准草案无异议。联系人：刘雪婧电话：0991-4336165 电子邮件：xjzccXYYC2024@163.com 地址：新疆乌鲁木齐市新市区西八家户路518号邮编：830054 02 质量标注公示稿乳鸽肉 Rugerou MUSCULUSCOLUMBAE باچكاگۆشى 本品为鸠鸽科动物原鸽ColumbaliviaGmelin.、家鸽ColumbaliviadomestiraGmelin.和岩鸽ColumbarupestrisPallas.的幼鸽除去头、爪、毛、内脏及肛门的新鲜全体。将30日龄内，重量300-500克的幼鸽屠宰，放血后除去头、爪、毛、内脏及肛门。切块鲜用或冷冻备用。【性状】本品为鲜品或冷冻品，呈不规则块状，皮肤淡黄色，具突起的毛孔；肌肉绛红色，具光泽，纤维状，肉质细腻；皮下脂肪少，白色至淡黄色。气微腥，无异味。【鉴别】（1）本品粉末（切块，混匀，绞碎）暗棕色至红棕色。肌纤维长条形，直径5~37µm，成束状或片状散在，淡黄色或近无色，有细密的波状纹理。脂肪油滴近无色，散在。（2）取本品，切块，混匀，绞碎，取约2g，加40%乙醇10ml，超声处理20分钟，滤过，取滤液作为供试品溶液。另取丙氨酸对照品，加40%乙醇制成每1ml含0.5mg的溶液，作为对照品溶液。照薄层色谱法（《中国药典》2020年版通则0502）试验，吸取供试品溶液4μl，对照品溶液1μl，分别点于同一硅胶G薄层板上，以正丁醇-冰醋酸-水（4:2:0.3）为展开剂，展开，取出，晾干，喷以茚三酮试液，在105℃加热至斑点显色清晰。供试品色谱中，在与对照品色谱相应的位置上，显相同颜色的斑点。【炮制】将30日龄内，重量300-500克的幼鸽屠宰，放血后除去头、爪、毛、内脏及肛门，切块鲜用或冷冻备用。【性质】二级干热，味咸。【功能与主治】生干生热，祛寒强筋，燥湿健肌，补虚强身，散气止痛。主治湿寒性或黏液质性疾病，如瘫痪，面瘫，颤抖症，全身虚弱，陈旧性头痛，关节痛，两胁痛等。【用法与用量】内服：根据病情食用或入制剂中。【贮藏】冷冻保存。驼峰油 Tuofengyou ADEPSCAMELE تۆگەلوكىسىمېيى 本品为双峰驼CamelusbactrianusL.驼峰脂肪，经炼制，滤过，冷却后而成的脂肪油。【性状】本品呈均匀脂状，乳白色，触之松软细腻，有微弱的特殊膻味。本品不溶于水，易溶于热乙醇，在石油醚（60~90℃）能缓慢溶解。熔点应为37～43℃（《中国药典》2020年版通则0612熔点测定第二法）。【检查】脂肪与脂肪油测定照脂肪与脂肪油测定法（《中国药典》2020年版通则0713）测定。酸值应不大于5.0 皂化值160.0～242.0 碘值25.0～57.0 【炮制】将驼峰脂切块，文火熬制至油渣呈干瘪黄色，滤过即得。【性质】味甘，性温。【功能与主治】补虚润燥，祛风活血，消肿解毒。主治体虚劳乏，肌肤不仁，皮肤瘙痒，筋肉挛急，疮疡肿毒，痔漏。【用法与用量】外用适量涂敷。【贮藏】置阴凉干燥处。绞股蓝 Jiaogulan HERBAGYNOSTEMMATISPENTAPHYLLI 本品为葫芦科植物绞股蓝Gynostemmapentaphyllum(Thunb.)Makino的干燥地上部分。夏、秋茎叶茂盛时，采割地上部分，除去杂质，水洗，干燥或切段、阴干。【性状】本品卷曲成团或成长段状。茎被短柔毛或近无毛，呈黄绿色或褐绿色，直径1-3mm，具细纵棱线，质韧，不易折断；卷须侧生于叶柄基部。叶互生，薄纸质或膜质，皱缩，易碎落，完整叶展开呈鸟足状复叶，边缘有锯齿。气微，微苦。【鉴别】（1）本品茎横切面：表皮细胞1列，扁平，外壁角质增厚，有时可见多细胞非腺毛；角隅处有厚角组织，常为4-6列细胞；皮层内有数个大小不等的维管束，断续排列成环；韧皮部外缘围绕有半月形纤维束；木质部导管圆形，多单个存在。髓部薄壁细胞中含淀粉粒。本品粉末灰绿色。腺毛头多由4个细胞组成，腺柄由1-2个细胞组成。非腺毛有多细胞和单细胞两种，多细胞非腺毛表面具角质线状纹理。气孔不定式，副卫细胞4-6个。具缘纹孔、螺纹或环纹导管。纤维多碎断，有的内含淀粉粒。淀粉粒椭圆形或不规则形，脐点点状、短缝状或人字形，层纹不明显。石细胞棕黄色，单个或成群散在，呈类方形、类长方形或纤维状，壁厚，层纹明显，孔沟清晰。（2）取本品粉末2g，加甲醇30ml，加热回流2小时，放冷，滤过，滤液蒸干，残渣加水20ml使溶解，用乙醚振摇提取2次，每次10ml，弃去乙醚液，水液用水饱和的正丁醇振摇提取3次，每次20ml，合并正丁醇提取液，蒸干，残渣加甲醇2ml使溶解，作为供试品溶液。另取绞股蓝对照药材2g，同法制成对照药材溶液。照薄层色谱法（《中国药典》2020年版通则0502）试验，吸取上述两种溶液各5l，分别点于同一硅胶G薄层板上，以环己烷-丙酮（2:1）为展开剂，展开，取出，晾干，喷以10%硫酸乙醇溶液，在105℃加热至斑点显色清晰，分别置日光和紫外光灯（365nm）下检视。供试品色谱中，在与对照药材色谱相应的位置上，显相同颜色的斑点或荧光斑点。【检查】水分不得过13.0%（《中国药典》2020年版通则0832第二法）。总灰分不得过13.0%（《中国药典》2020年版通则2302）。酸不溶性灰分不得过2.0%（《中国药典》2020年版通则2302）。【浸出物】照水溶性浸出物测定法（《中国药典》2020年版通则2201）项下的冷浸法测定，不得少于14.0%。饮片【炮制】除去杂质，洗净，切段，干燥。【性状】本品为不规则的段，根茎、茎、叶、花混合。根茎细，圆柱形。茎细弱，具纵棱，黄绿色或黄白色，卷须生于叶腋，纤细，2歧或单一。叶片多皱缩或破碎，薄纸质或膜质，完整者通常由5-7小叶组成鸟趾状复叶，小叶片边缘具锯齿，黄绿色，两面疏被短毛，有极短的小叶柄。气微，味微苦、微甘。【鉴别】【检查】同药材。【浸出物】照水溶性浸出物测定法（《中国药典》2020年版通则2201）项下的冷浸法测定，不得少于13.0%。【性味与归经】苦、微甘、寒。归肺、脾、肾经。【功能与主治】清热解毒，止咳祛痰，益气养阴。用于胸膈痞闷，痰阻咳嗽，心悸气短，眩晕头痛，健忘耳鸣，自汗乏力。【用法与用量】6～10g。【贮藏】置通风干燥处。

100 项与绞股蓝提取物相关的药物交易

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