This work addresses the challenge of detecting trace levels of antidiabetic drugs like metformin (Met) by developing novel surface molecular-imprinted fluorinated covalent organic framework nanocatalytic probes (MoS₂@FCOF and SNP@FCOF) leveraging the piezo-triboelectric effect. Probes were synthesized on sulfur nanoparticles (SNP) or molybdenum disulfide (MoS₂) substrates using Met as the template molecule, with 4',4''-(1,3,5-triazine-2,4,6-triyl)trianiline, terephthalaldehyde, and 2,3,5,6-tetrafluoroterephthalaldehyde as monomers. Specific recognition of Met was achieved through spatially matched molecularly imprinted cavities engineered within the FCOF probe. It was found that the MoS₂@FCOF and SNP@FCOF probes exhibit strongly ultrasonic piezo-triboelectric nanocatalytic activity for the oxidizing 3,3',5,5'-tetramethylbenzidine (TMB) that can be monitored with surface-enhanced Raman scattering (SERS) and fluorescence (FL) techniques. The intrinsic piezo-triboelectric nanocatalytic properties were systematically investigated through slope method and slope ratio procedures. The nanocatalytic performance enhanced 2030 % by means of piezo-triboelectric hybrid nanogenerator (PTEG). Based on this strategy, a dual-mode SERS/FL method was developed for 0.02-2.0 nM and 0.05-1.8 nM Met, with detection limits of 0.008 nM and 0.016 nM respectively. This method is also applicable for the determination of biguanide drugs such as phenformin, buformin, and proguanil, with detection limits of 0.12, 0.16, and 0.23 nM, respectively. The nanoprobes demonstrated high selectivity (imprinting factor = 6.91) against 1000-fold concentration interferents, achieving satisfactory recoveries (92.1 %-107.2 %) in river water, urine and pharmaceutical formulations analysis.

2025-01-01·Endocrine Metabolic & Immune Disorders-Drug Targets

Sustainable Synthesis of Guanidine Derivatives and Computational Assessment of their Antidiabetic Efficacy

Article

作者: Rudithongru, Lemzila ; Nakro, Vevosa ; Ao, Imkongyanger ; Richa, Kikoleho ; Ao, Ketiyala ; Lotha, Tsenbeni N. ; Namsa, Nima D. ; Pankaj, Pranay Punj ; Jamir, Latonglila ; Ritse, Vimha ; Sinha, Upasana Bora

Background::

Type 2 Diabetes Mellitus (T2DM) represents a significant and pressing worldwide health concern, necessitating the quest for enhanced antidiabetic pharmaceuticals. Guanidine derivatives, notably metformin and buformin, have emerged as pivotal therapeutic agents for T2DM management.

Aims::

The present study introduces an efficient one-pot synthesis method for the production of symmetrical guanidine compounds and subsequently, their evaluation as potential T2DM agents.

Methods::

This synthesis involves the reaction of isothiocyanates with secondary amines, employing an environmentally friendly and recyclable reagent, tetrabutylphosphonium tribromide (TBPTB). In order to understand the mechanics of ligand-protein interaction, ADME/Toxicity, and drug-likeliness aspects, in silico studies were incorporated.

Results::

An efficient and easy method for synthesis of guanidine compounds has been devised. Comprehensive assessment of the biological activity of the synthesized guanidine compounds, specifically in the context of T2DM, has been rigorously conducted.

Conclusion::

Computational analyses have unveiled their substantial potential as promising antidiabetic agents. Results highlight the relevance of these compounds in the ongoing pursuit of novel therapeutic solutions for T2DM.

2025-01-01·JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Non-targeted screening of illegal added hypoglycemic drugs and their derivatives in functional foods using characteristic fragment ions scanning based on liquid chromatography-high-resolution mass spectrometry

Article

作者: Liu, Yajie ; Feng, Feng ; Zhang, Feng ; Du, Rongzhu ; Wang, Xiujuan ; Feng, Xuesong

Functional foods that are illegally adulterated with chemical hypoglycemic drugs or their derivatives pose serious risks to human health. However, the detection of these compounds is a challenge due to the unknown nature of their structures, particularly as many of these compounds are newly synthesized and lack standardized references. In this study, we developed a non-targeted screening strategy for detecting illegally added hypoglycemic drugs and their derivatives in oral solution and hard capsule functional foods. This strategy utilizes a self-established characteristic fragment ions mass spectrum database based on ultrahigh-performance liquid chromatography-quadrupole/orbitrap high-resolution mass spectrometry. Nine characteristic fragment ions were identified by analyzing the mass spectrometric fragmentation patterns of hypoglycemic drugs. Meanwhile, the precursor and fragment ions of 38 known hypoglycemic drugs were collected and incorporated into the mass database. The detection limits of hypoglycemic drugs in oral solution and hard capsule samples were 0.01-10 μg/L and 0.01-50 μg/kg, respectively. Applying the non-targeted method to 20 batches of suspicious samples, we found that 3 batches of samples contained illegal added drugs. Furthermore, we identified a previously unrecorded hypoglycemic drug not present in the mass database. These results indicate that the developed strategy is a powerful tool for the rapid and highly sensitive identification of both known and unknown hypoglycemic drugs, as well as their derivatives in functional foods.

100 项与盐酸丁福明相关的药物交易

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