The retentivity and enantioselectivity of chiral HPLC columns containing immobilized stereoselective proteins as the stationary phase were investigated by varying the parameters of sample size, and inorganic buffer concentrationColumns containing bovine serum albumin, ovomucoid, and α1-acid glycoprotein were evaluated and retention characteristics were compared by separating acidic and basic test compoundsEnantioselectivity of each of these protein-based chiral columns is affected somewhat differently by the chromatog. parameters; chiral separations were optimized by adjustment of the appropriate parameters.