The effect adding acid-soluble wheat protein (ASP), mainly composed of gliadin, to wheat flour and rice flour blend bread in mechanized bread-making was investigated using the following method. Rice flour was used to replace 40% of the wheat flour, and wheat protein (gluten and ASP) was added to constitute 10% of the total mixture, thus replacing 20% to 40% of the gluten with ASP. In the sponge and dough method, the addition of ASP improved the gas holding capacity of the sponge, and the dough development time shortened during the mixing process. In addition, molding conditions for mass production were improved due the suppression of changes in dough rheol. properties caused by the time lag during the dividing process. Microstructural observation of sheeted dough showed that the gluten network of the dough extended in a uniform direction. Consequently, it was shown that the addition of ASP resulted in increased extensibility of the dough. As a result, the addition of ASP improved uneven crumb structure after baking and decreased the rate of crumb firmness during storage (20°C, 24-96 h). In conclusion, the addition of ASP improved the stabilization of the bread-making process and increased the product quality of wheat flour and rice flour blend bread m mechanized bread-making.