Objective To improve the quality standard of Qingre Zhili MixtureMethods Thin-layer chromatog.( TLC) method was used for qual. identification of Qingre Zhili MixtureHigh-performance liquid chromatog.( HPLC) method was used for the content determination of gallic acid, puerarin, paeoniflorin and baicalin in this preparationResults The TLC spots of Radix Pulsatillae, Acacia catechu, Cortex Phellodendri, Rhizoma Coptidis and Radix Scutellariae were clear, the separation was good, and the specificity was strong.Gallic acid, puerarin, paeoniflorin and baicalin showed good linear relationships in the ranges of 4. 557 1-41. 014 3 μg/m L, 3. 450 0-31. 050 0 μg/m L, 10. 907 1-98. 164 3 μg/m L and 18. 500 0-166. 500 0 μg/m L( r ≥ 0. 999 4), resp.The average recoveries were 100. 43%, 100. 71%, 98. 28% and 100. 65% with RSDs of 0. 86%, 1. 60%, 0. 84% and 1. 16%( n = 6).Conclusion This method is accurate, reliable and reproducible, which can be used for the quality control of Qingre Zhili Mixture
