作者: K, Gowtham ; Neog, Kartik ; Moorthy, S Manthira ; Singh, Abhishek ; Gadad, Hanamant ; Deepika, Iyyangar ; Ponnuvel, Kangayam M ; Shukla, Pawan ; Gnanasekaran, Roja ; Kumar, Indira ; Arunkumar, K P ; Subramanya, H S ; Dubey, Himanshu ; Ghosh, Sanjay ; Debnath, Rajal ; Santhoshkumar, Rashmi ; Sivaprasad, Vankadara ; Subrahmanyam, Gangavarapu ; Sangannavar, Prashanth ; Sivakumar, G ; Kumar, Vikram

A potential viral pathogen, cypovirus causing cytoplasmic polyhedrosis in the Indian golden muga silkworm, Antheraea assamensis Helfer, was isolated and characterized. Electron microscopy analysis confirmed the presence of typical occlusion bodies with icosahedral virions embedded in polyhedral matrices. The viral genome was dsRNA totaling approximately 24.9 kb in size, divided into 10 discrete segments containing one single open reading frame (ORF) each. Genomic analysis revealed conserved non-coding sequences such as 'AGUAAU' and 'AUAGAGC' at 5' and 3' termini, respectively. Functional annotation identified the presence of structural proteins, such as major and minor capsid proteins, as well as enzymatic proteins, including RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis using deduced amino acid sequences of viral RdRp gene indicated a close evolutionary relationship between the cypovirus isolated from Antheraea assamensis and Antheraea mylitta cypovirus 4 (AmCPV4), thus we named the Indian golden muga silkworm cypovirus as Antheraea assamensis cypovirus 4 (AaCPV4). Nevertheless, AaCPV4 does not have a genomic segment 11 which was earlier reported in AmCPV4. Bioassay studies demonstrated high virulence, with 100 % larval mortality observed within 12 days of infection due to cytoplasmic polyhedrosis. Tissue tropism studies revealed significant viral replication in the midgut, hemolymph, and malpighian tubules, with the midgut exhibiting the highest viral load (9.65 log copy number of RdRp gene/µg of RNA). Cross-transmission experiments demonstrated AaCPV4 host specificity, infecting only in Antheraea species (A. mylitta and A. assamensis), while other lepidopteran insects including silkworms Bombyx mori, and Samia ricini were not susceptible to AaCPV4 infection. According to morphological, genetic, and biological characteristics, we suggest that AaCPV4 is an isolate of the species Cypovirus antheraeae (family Spinareoviridae).

2025-06-01·Gene

Construction and characterization of cloning vector and temperature sensitive vectors for Gordonia sp. IITR100

Article

作者: Chandrakanta, Chudamani ; Shefrin, Seyad ; Jain, Prarthana ; Karmakar, Shreyoshi ; Srivastava, Preeti ; Kumar, Vipul ; Sundar, Durai

Gordonia spp. are Gram-positive, non-sporulating bacteria which have several industrial as well as environmental applications. In order to enhance their potential, metabolic engineering is required which often involves genome manipulation. For this purpose, temperature sensitive plasmids are useful as the desired genes for integrases or recombinases can be supplied transiently, followed by curing of the plasmid. Here, we report the construction of a cloning vector and temperature sensitive vectors based upon the pKB1 replicon from Gordonia westfalica. The amino acid residues or regions for creating temperature sensitive mutants were predicted based on in silico methods, and the dynamics of these mutant proteins were studied using docking and molecular dynamic simulations. The desired mutations were incorporated in the replication protein by site directed mutagenesis. The results were validated by growth kinetics of the wild type and mutants at permissive and non-permissive temperatures. This is the first report on temperature sensitive vectors based on native replicon from a plasmid originating from Gordonia.

2025-02-01·International Immunopharmacology

Global transcriptome profiling of ST09 treated breast cancer cells identifies miR-197-5p/GPX3 antioxidant axis as a regulator of tumorigenesis

Article

作者: Ravindran, Febina ; Thumsi, Jayanthi ; Shahana, M V ; Choudhary, Bibha ; Mhatre, Anisha ; Nirgude, Snehal ; Sharma, Shivangi ; Rai, Prashant Kumar ; Desai, Sagar ; Mahadeva, Raghunandan

ROS (Reactive Oxygen Species) has a dual role in tumorigenesis. Some cancers have high ROS conditions, and others have low ROS. TNBC thrives on high ROS compared to other Breast Cancer subtypes. Several antioxidant enzymes catalyze the detoxification of reactive oxygen species and prevent free radicals from damaging DNA and accumulation of mutation. Curcumin, a polyphenol dietary supplement, acts as a potent antioxidant, is known to reduce inflammation, and has anticancer properties. Here, we aim to understand alterations in the transcriptome (miRNA and mRNA expression) induced by ST09 in breast cancer cell lines. We identified an antioxidant system that is upregulated in breast cancer cell lines. Among the antioxidant enzymes regulated by miRNA was GPX3. A novel miRNA-mRNA antioxidant axis, miR-197-5p/GPX3, was observed in the TNBC cell line. We further validated the regulation of GPX3 by miRNA using luciferase assay. GPX3 overexpression, knockdown, and activity assay indicated the anti-tumorigenic role of GPX3 in the TNBC cell line. Further, treatment of TNBC xenograft with ST09 showed tumor reduction in vivo. ST09 potentiates the effect of standard-of-care (SOC) drug Cisplatin in vivo. ST09 can be exploited as a single chemotherapeutic agent or in combination treatment modalities, reducing the dosage of potent drugs.

项与 Institute of Bioinformatics & Applied Biotechnology 相关的新闻（医药）

2005-10-19

·BioSpace

Dharam Singh Promises Rs. 10 Crores For Biotech Park

The Chief Minister, Dharam Singh, said here on Sunday that the State Government, while being committed to the welfare of farmers and the poor, would accord high priority to information technology and biotechnology, as had been done by the previous Congress Government. Inaugurating the fourth biotechnology trade show of the State, Bangalore Bio 2004, Mr. Singh promised an initial investment of Rs. 10 crores for the proposed biotechnology park, Bangalore Helix, on the outskirts of the city. The money would be used to upgrade infrastructure of the Institute of Biotechnology and Applied Biology and the Centre for Human Genetics, both started with partial State assistance. About Rs. 3 crores had already been released, and the Centre had been requested to provide Rs. 50 crores for the park, he added. The institute is to be shifted to phase 1 of the biotechnology park, which will come up on 40 hectares of land adjacent to the Electronics City. It offers a three-semester postgraduate programme in bioinformatics, including a six-month internship in the industry.

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