4-week continuous skin environmental improvement test of Blue rose extract powder capsule and placebo products - 4-week continuous skin environmental improvement test of Blue rose extract powder capsule and placebo products

开始日期2023-03-03

申办/合作机构

Kinjirushi Co. Ltd.

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项与 Kinjirushi Co. Ltd. 相关的文献（医药）

2023-06-01·Breeding science

Allyl isothiocyanate and 6-(methylsulfinyl) hexyl isothiocyanate contents vary among wild and cultivated wasabi (Eutrema japonium).

Article

作者: Murayama, Seiji ; Kobayashi, Keiko ; Yamada-Kato, Tomoe ; Ishida, Kaori ; Yamane, Kyoko ; Okunishi, Isao ; Haga, Natsuko

Wasabi (Japanese horseradish, Eutrema japonicum) is the only cultivated species in the genus Eutrema with functional components that provide a strong pungent flavor. To evaluate genetic resources for wasabi breeding, we surveyed variations in the two most abundant isothiocyanate (ITC) components in wasabi, allyl isothiocyanate (AITC) and 6-methylsulfinyl (hexyl) isothiocyanate (6-MSITC, hexaraphane). We also examined the phylogenetic relationships among 36 accessions of wild and cultivated wasabi in Japan using chloroplast DNA analysis. Our results showed that (i) the 6-MSITC content in currently cultivated wasabi accessions was significantly higher than in escaped cultivars, whereas the AITC content was not significantly different. (ii) Additionally, the 6-MSITC content in cultivated wasabi was significantly lower in the spring than during other seasons. This result suggested that the 6-MSITC content responds to environmental conditions. (iii) The phylogenetic position and the 6-MSITC content of accessions from Rebun, Hokkaido Prefecture had different profiles compared with those from southern Honshu, Japan, indicating heterogeneity of the Rebun populations from other Japanese wasabi accessions. (iv) The total content of AITC and 6-MSITC in cultivated wasabi was significantly higher than that of wild wasabi. In conclusion, old cultivars or landraces of wasabi, "zairai", are the most suitable candidates for immediate use as genetic resources.

2022-11-01·Free Radical Biology and Medicine

Establishment of Neh2-Cre:tdTomato reporter mouse for monitoring the exposure history to electrophilic stress

Review

作者: Kitamura, Hiroshi ; Yamamoto, Masayuki ; Katori, Yukio ; Murakami, Shohei ; Oishi, Tetsuya ; Yamada-Kato, Tomoe ; Okunishi, Isao ; Motohashi, Hozumi

Cells are often exposed to exogenous and endogenous redox disturbances and exert their protective mechanisms in response to stimuli. The KEAP1-NRF2 system plays pivotal roles in counteracting oxidative damage. Due to the transient nature of NRF2 activation, the identification of cells in which NRF2 is activated in response to systemic stimuli is sometimes not easy. To examine the electrophilic stress response at a single-cell resolution, we aimed to develop a new reporter mouse in this study. A cell-tracing strategy exploiting Cre recombinase-mediated activation of a reporter gene was chosen for stable detection of reporter expression instead of real-time monitoring of the cellular response. We established a transgenic mouse line expressing the Neh2-Cre recombinase fusion protein. As Neh2 is an amino-terminal domain of NRF2 that serves as a degron and mediates KEAP1-dependent degradation and electrophile-inducible stabilization, Neh2-Cre was expected to be activated in response to electrophiles. The Neh2-Cre transgenic mouse was crossed with the ROSA26-loxP-stop-loxP-tdTomato reporter mouse (ROSA-LSL-tdTomato mouse). The compound mutant reporter mice exhibited accumulation of tdTomato-positive cells in various organs after repeated administration of CDDO-Im, one of the NRF2-inducing electrophiles. The mice were also successfully used for the detection of cells that experienced a cisplatin-induced electrophilic stress response.

2021-01-01·Food Science and Technology Research

Assessment of mutagenicity, acute and sub-acute toxicity and human trial safety of wasabi leaf extract powder

作者: Miura, Yousuke ; Hira, Yoshitada ; Hou, De-Xing ; Nakamura, Yo ; Okunishi, Isao

Wasabi leaf has been reported to show human health benefits without assessment of its safety.This study aims to investigate the mutagenicity, acute and sub-acute toxicity and human trial safety of wasabi leafext. (WLE).The Ames test was used to assess mutagenicity, while acute and sub-acute toxicity were assessed by oral administration in five-week-old SIctlCR mice (SPF) and five-week-old Sprague-Dawley (SD) rats, resp.Human trial safety was further determined in a clin. trial.Twelve healthy subjects, aged 20-64 years and mildly obese (BM1 23.0 to 30.0 kg/m2), were enrolled in the clin. trial, and participants ingested 200 mg WLE daily for 12 wk.The effect of WLE on fat metabolism was evaluated by visceral fat area (VFA), s.c. fat area (SFA), VFA/SFA(V/S) area ratio, body weight, BMI, TC, T-Cho, HDL-C, LDL-C, waist circumference, and body fat percentage.In the Amcs test, WLE did not show mutagenicity in the range of 1.2-5000 pg/plate.No acute toxicity was observed in Slc:ICR mice (SPF) administered 5000 mg/kg/day WLE, and no sub-acute toxicity was observed in CrltCD (SD) rats administered 2500 mg/kg/day WLE.In the human clin. trial, there were no significant differences between the WLE and placebo groups for any outcome measure assessed.Thus, ingestion of200 mg/day of WLE was demonstrated for the first time to be safe.Taken together, our data on the mutagenicity, acute and sub-acute toxicity and human trial safety of WLE provide the first standard references for wasabi leaf supplement application.

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