In an operative room, medical images serve as intraoperative navigation or support for surgeons to ensure safe surgery. However, conventional two-dimensional images from drip infusion cholangiography with CT (DIC-CT) or magnetic resonance cholangiopancreatography (MRCP) are difficult to understand the anatomy intuitively. Then, we have developed a virtual reality (VR)/mixed reality (MR) system to offer a noninvasive laparoscopic surgery as well as to minimize serious complications. We also performed the MR-guided surgery with favorable outcome. In MR-guided laparoscopic cholecystectomy, using HoloLens can display precise three-dimensional models of the cystic duct, biliary tree, cystic artery, and hepatic artery stably on the real visual field as hologram.