Objective Elevation of postprandial blood triglyceride levels are associated with increased risk of cardiovascular diseases. Some specific dietary fibers are known to suppress postprandial blood triglyceride elevation, however, the effect of tomato-derived dietary fiber (TDF) on postprandial blood triglyceride levels remains to be elucidated. Methods A placebo-controlled, crossover study was conducted. TDF-fortified beverage (T, 1.6 g of TDF) and placebo (P, without TDF) were used. Seventy-nine healthy subjects consumed either of the two beverages with eating high-fat diet (total fat: 42.3 g). Blood triglyceride levels before meals and, 2, 3, 4, 6 h after meals were measured, and the maximum concentration of Δblood triglyceride levels (ΔCmax), and the incremental area under the curve (IAUC) for 6 h were calculated Results The anal. in all subjects showed T did not significantly suppress blood triglyceride levels at each time point, ΔCmax, and IAUC. Subgroup anal. in subjects with a Cmax of 200 mg/dL or more showed that T significantly suppressed blood triglyceride levels at 4 h, ΔCmax, and IAUC compared with P (P<0.01, P<0.01, P<0.01, resp.). Conclusions The clin. study shows TDF may attenuate the postprandial rise of blood triglyceride in subjects with a high postprandial blood triglyceride level.