A prospective, comparative, randomized, parallel arm, open label, control group study toevaluate the safety and efficacy of Botroclot in surgical wound closure in C-Section

开始日期2023-05-30

申办/合作机构

Jagdale Industries Pvt Ltd.

CTRI/2023/05/052517 / CompletedN/A

A prospective, multi-centre, randomized, parallel arm, open label, control groupstudy to evaluate the efficacy of Mulmina Apple in children with mild tomoderate Attention Deficit Hyperactivity Disorder (ADHD).

开始日期2023-05-15

申办/合作机构

Jagdale Industries Pvt Ltd.

CTRI/2023/03/050888 / Recruiting临床3期

A Prospective, Open-Label, Multi-Centred, Comparative, Randomized Controlled Trial to Evaluate The Safety And Efficacy of Botroclot In Diabetic Foot Ulcers

开始日期2023-03-24

申办/合作机构

Jagdale Industries Pvt Ltd.

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2020-10-01·3 Biotech4区 · 工程技术

Botrops derived hemocoagulase formulation a probable agent for diabetic wound healing

4区 · 工程技术

Article

作者: Jagdale, Rajesh N ; Gourishetti, Karthik ; Keni, Raghuvir ; Kinra, Manas ; Ahmed, Syed Mushtaq ; Raghavendra, K V ; Nayak, Pawan G ; Shenoy, Rekha ; Nandakumar, Krishnadas

AbstractBotroclot is a marketed preparation containing hemocoagulase, which is an enzyme having coagulant activity, isolated from the snake Botrops atrox. This formulation is used in dental surgeries and other minor surgical wounds. However, the formulation remains untested in diabetic wounds. Hence, we proposed a study for the topical application of Botroclot in high-fat diet (HFD) + Streptozotocin (STZ) induced diabetic rats. HFD was fed initially to rats which facilitates the development of insulin resistance. Thereafter, an injection of STZ (40 mg/kg, i.p.) was given. This resulted in the development of diabetes with elevated fasting glucose and impaired glucose tolerance. After stabilization of blood glucose values, wounds were created by punch biopsy on the dorsal side of the palm of the rat to mimic the diabetic wounds frequently seen in the case of humans. Later, the application of Botroclot on these wounds was carried out for 15 days. Topical application of hemocoagulase improved the wound closure and there was a gradual decrease in inflammatory markers and a substantial increase in collagen deposition occurred. Histopathological findings indicated the same, with an increase in granulation tissue suggesting that the topical application moderately improves the wound healing in diabetic rats. We conclude that Botroclot can have a mild to moderate effect in improving collagen deposition and thus wound contraction, improving wound closure in diabetic wounds in rats. This study also establishes the basis for exploration of agents from venom-based sources in diabetic wound healing.

2016-12-01·Toxicon3区 · 医学

Serine proteinases from Bothrops snake venom activates PI3K/Akt mediated angiogenesis

3区 · 医学

Article

作者: Ullah, Anwar ; Masood, Rehana ; Bhat, Shreesha K ; Arni, Raghuvir K ; Joshi, Manjunath B ; Biligiri, Setlur G ; Satyamoorthy, Kapaettu

The discovery of rapid acting and powerful angiogenic proteins are of significant interest in the treatment of various human disorders associated with insufficient angiogenesis such as ischemia, menorrhagia and delayed wound healing. Snake venoms consist of a mixture of bioactive proteins and polypeptides and are rich sources of pharmacologically important molecules. Serine proteinases are one of the abundant proteins present in Bothrops snake venoms and possess multiple biological functions including the regulation of the blood coagulation cascade. In this study, serine proteinases from Bothrops atrox (B. atrox) and Bothrops brazili (B. brazili) that modulate angiogenesis were purified and characterized. Molecular size exclusion chromatography, affinity chromatography followed by ion exchange chromatography of the serine proteinases indicated molecular masses of around 32 kDa. Serine proteinases from both the species exhibited diverse catalytic activities such as the ability to induce amidolytic, fibrinogenolytic, gelatinolytic activities and also coagulated plasma with a minimal coagulation concentration of 2.4 μg/mL. Serine proteinases facilitated the sprouting of human umbilical vein endothelial cells (HUVEC) in three-dimensional culture systems and induced tubule formation in monolayer culture systems. Serine proteinase stimulated Akt^ser473 and eNOS^ser1177 phosphorylation in endothelial cells and addition of PI3K inhibitor LY294002 abrogated the effects of serine proteinases on sprout formation of endothelial cells in 3D collagen gels, suggesting that serine proteinase facilitated angiogenesis was mediated by PI3K/eNOS signaling axis. We also show in agarose plug assays using a mouse model, serine proteinases from Bothrops venoms significantly enhanced neovascularization. Our data suggests pro-angiogenic activity by the serine proteinases from B. atrox and B. brazili venom and further studies are warranted to explore the therapeutic applications.

Indian Drugs

Synthesis of sodium carboxymethyl guar and its characterization

作者: Thampi, P. P. ; Paranjothy, K. L. K.

Sodium chloroacetate (20 g) was added to a mixture of guar gum (10 g) and 1:1 aqueous iso-Pr alc. (40 mL) to give a slurry which was heated to 60° for 2 h; the product was filtered and washed with an 80:20 aqueous MeOH mixture and adjusted to pH 7.Properties of sodium carboxymethyl guar gum are more controllable than guar gum with respect to thickening, suspending, gel formation and emulsification.The process described here is suitable for bulk manufactureSodium carboxymethyl guar gum prepared by this method was nontoxic in mice up to 10,000 mg/kg.

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