The objective of this paper is to set up a method for determining the content of matrine and oxymatrine simultaneously in Yunxiangjing.HPLC method was used with Agilent ZORBAX-NH2 column (4.6 mm X 250 mm, 5 μm).The mobile phase was anhydrous ethanol-acetonitrile-3%phosphoric acid solution (9:82:9), and the detecting wavelength was at 210 nm.The results showed that the linearity of matrine and oxymatrine was in the range of 0.08-0.60 μg and 0.30-2.25 μg, resp.The average recovery was 100.09% and 100.10%, resp.RSD was 1.50% and 1.55%, resp.It was concluded that this method is accurate and reliable with good reproducibility, and can be used for the simultaneous determination of matrine and oxymatrine in Yunxiangjing to control the quality.