Zavod Republike Slovenije ZA Transfuzijsko Medicino (Zavod Republike Slovenije ZA Transfuzijsko Medicino)

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关联

1

项与 Zavod Republike Slovenije ZA Transfuzijsko Medicino 相关的药物

M1

靶点

MIF

作用机制

MIF抑制剂

在研机构-

原研机构

Zavod Republike Slovenije ZA Transfuzijsko Medicino

在研适应症-

非在研适应症

炎症

最高研发阶段无进展

首次获批国家/地区-

首次获批日期-

1

项与 Zavod Republike Slovenije ZA Transfuzijsko Medicino 相关的临床试验

NCT01436019

/ Unknown statusN/A

Analysis of Anti-infliximab, Anti-adalimumab and Anti-etanercept Antibodies in Children and Young Adolescents With Juvenile Idiopathic Arthritis

The purpose of this study is to analyze the frequency of the formation of antibodies against three different anti-TNF biologic agents used for the therapy of juvenile idiopathic arthritis.

开始日期2011-10-01

申办/合作机构

Zavod Republike Slovenije ZA Transfuzijsko Medicino

[+1]

100 项与 Zavod Republike Slovenije ZA Transfuzijsko Medicino 相关的临床结果

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0 项与 Zavod Republike Slovenije ZA Transfuzijsko Medicino 相关的专利（医药）

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30

项与 Zavod Republike Slovenije ZA Transfuzijsko Medicino 相关的文献（医药）

2025-06-01·Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

Catalytic proteasome subunits are heterogeneously active in human platelets with β1i playing a role in signaling and granule release

Letter

作者: Gobec, Martina ; Stanišić, Slavica ; Smrdel, Lara ; Zajec, Živa

Human platelets contain catalytically active subunits of both constitutive and immunoproteasomes, with predominant expression of β5i, β5c, β1i, and β1c subunits.Anal. of 31 healthy donors revealed substantial interindividual variability in both the protein levels and active states of proteasome subunits in platelets.The proteasome catalytic subunit β1i was found to play a significant role in regulating platelet signaling pathways, particularly influencing the p38 and NFB pathway.Inhibition of β1i enhanced α-granule release, notably increasing CCL5 secretion, upon ADP activation, highlighting its potential role in platelet activation and immune regulation.

2024-11-01·Journal of Immunological Methods

Optimizing the method for expressing human monoclonal antibodies from a single peripheral blood cell from vaccinated donors

Article

作者: Omejec, Sandra ; Tompa, Manuela ; Šerbec, Vladka Čurin ; Kovač, Valerija

Human monoclonal antibodies are essential diagnostic and research tools and one of the most promising therapeutics. In the past years, single B cell technologies have evolved and over-come conventional methods' limitations, enabling the isolation of scarce B cell populations with desirable characteristics. In this study, we describe a simple and efficient method to isolate anti-gen-specific plasmablasts and memory B cells from hepatitis B virus vaccinated donors' peripheral blood and consequently amplification of immunoglobulin variable region genes. Amplified immunoglobulin variable region genes were cloned into expression vectors and transfected into a human cell line to produce human recombinant monoclonal antibodies. Major challenges in this protocol were isolation of antigen-specific B cells based on surface markers, recovering mRNA from a single cell for efficient amplification, and cloning the correct insert into a desired expression vector. The essential feature of our protocol was the separation of B cells from peripheral blood mononuclear cells before sorting. We identified antigen-specific binding B cells based on the expression of surface markers CD19, CD27, IgG, and binding to hepatitis B surface antigen. Efficient single-cell reverse transcription and polymerase chain reaction (RT-PCR) were achieved using a random primer mix and Kapa Hifi Hot Start Polymerase. Amplification efficiency differed among heavy and light chain variable regions (highest at heavy chain (68 %) and lowest at lambda light chain (22 %)). After co-transfection of HEK293T/17 with successfully cloned heavy and light chain vectors, 70 % of transfected cells produced recombinant monoclonal antibodies at a concentration ∼ 4 μg/ml and 7 % of them showed binding to HBsAg. Human monoclonal antibodies from peripheral blood have advantages over antibodies of mouse origin or phage display libraries, because of their high specificity and self-tolerance. Using the described protocol, we can generate fully human monoclonal antibodies to any other antigen for application in immunotherapy or basic research.

2021-10-01·International Immunopharmacology2区 · 医学

Programmed death ligand 1 (PD-L1) plays a vital part in DC tolerogenicity induced by IFN-γ

2区 · 医学

Article

作者: Rožman, Primož ; Švajger, Urban ; Tešić, Nataša

Interferon-γ (IFN-γ) is the sole representative of type II IFNs, with well recognized role in numerous inflammatory processes. Lately, its significant pleiotropic nature has been recognized in many scenarios, where IFN-γ contributes to maintenance or induction of tolerogenic responses in context of various immune cell types. In this manuscript we demonstrate, that IFN-γ-mediated induction of programmed death ligand 1 (PD-L1) on human monocyte-derived dendritic cells (DCs) represents an important tolerogenic aspect in immunological network of type II IFNs. When fully differentiated, immature DCs were treated with increasing concentrations of IFN-γ there was no sign of maturation, as revealed by CD80, CD83 and CD86 expression. In terms of co-stimulatory receptor response, we did observe a dose-dependent increase in CD40 expression. Phenotypic analysis of inhibitory molecules revealed that PD-L1 expression is particularly sensitive to IFN-γ, as its expression can be induced almost 10-fold in comparison to non-treated DCs. Functional analysis of such PD-L1^high DCs revealed significant immunosuppressive properties in a mixed lymphocyte reaction with whole or memory CD4⁺ T cells. When IFN-γ treated DCs were co-cultured with naive CD4⁺CD45RA⁺ T cells, they induced an increased percentage of CD4⁺CD25⁺CD127^-FoxP3⁺ Tregs. Inhibition of PD-1/PD-L1 axis using neutralizing anti-PD-L1 mAbs, reversed the immunosuppressive effect of IFN-γ-treated DCs to suppress CD4⁺ T cell proliferation and to induce Tregs. In summary, our findings demonstrate the importance of IFN-γ-mediated tolerogenic effects, exerted on DCs by inducing increased expression of PD-L1, which enhances their regulatory function.

100 项与 Zavod Republike Slovenije ZA Transfuzijsko Medicino 相关的药物交易

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100 项与 Zavod Republike Slovenije ZA Transfuzijsko Medicino 相关的转化医学

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