To establish a HPLC method for the content determination of ginsenoside Rg1 in Gucining tablets, the HPLC was performed on a Merck C18 (250 mm × 4.6 mm)chromatog. column gradient eluted with acetonitrile-water at the flow rate of 1.0 mL/min.The detection wavelength was set at 203 nm.Results showed that the linear response range was 0.201-0.603 μg/mL (r = 0.9994).The mean recovery was 100.3% (n=9), with RSD = 1.34%.This method was simple, quick, and accurate.Therefore it was suitable for the quality control of Gucining tablets.