PURPOSEThe aim of this study was to identify predictive factors for spontaneous internal limiting membrane (ILM) peeling (SP) after idiopathic epiretinal membrane (ERM) removal and to compare outcomes between patients with and without SP.DESIGNThe PEELING study was a national randomized clinical trial.PARTICIPANTSPatients with symptomatic idiopathic ERM were recruited from five ophthalmology departments.INTERVENTIONVitrectomy and ERM dissection were performed. When the ILM spontaneously peeled off over an area of at least two optic disc diameters around the fovea, patients were not randomized and were included in the spontaneous ILM peeling group (SPG). Otherwise, patients were randomized intraoperatively to either the no ILM peeling group (NPG) or the active ILM peeling group (APG).MAIN OUTCOME MEASURESMicroperimetry, best-corrected visual acuity (BCVA) measurements and optical coherence tomography findings were assessed at month 1 (M1), M6, M12. The primary outcome was the difference in microscotoma number between baseline and M6.RESULTSOf 213 patients, 101 experienced SP and 100 were randomized (APG, n=51 and NPG, n=49). In the SPG, 99 patients were included in the baseline characteristics analysis and 75 in the follow-up characteristics analyzes. Baseline characteristics were similar between all groups. The difference in microscotomas number between baseline and M6 was not statistically significant between groups (-4.8 ±9.9 in NPG,-2.2 ±7.3, in APG and -2.7 ±6.9 in SPG). At M1, the difference in microscotoma number was significantly higher in the APG (+1.6 ±8.9) than in the SPG (-1.6 ±6.2, p <0.001) and NPG (-2.1 ± 10.2, p=0.006). The BCVA was significantly better in the SPG compared to the APG at M1 (p <0.01) and M6 (p=0.03) and to the NPG at M6 (p <0.01) and M12 (p=0.01). The anatomical ERM recurrence rate was lower in the SPG (4%, n=3) than in the NPG (19.6%, n=9, p=0.0096) but similar between the SPG and APG (0%). Two patients in the NPG underwent revision surgery.CONCLUSIONThe difference in microscotomas number between baseline and M6 was not statistically significant between groups. Spontaneous ILM peeling was common and associated with better clinical outcomes. No predictive factors were identified.