Research utilization: Integrated healthcare with non-medical caregiver support to improve continuous primary care for elders who need assistance (WinCare 2.0) within the urban context in health promoting hospitals transferred to provincial administrative organizations

开始日期2025-04-21

申办/合作机构

Phramongkutklao College of Medicine

TCTR20250322001

/ Not yet recruiting临床2期IIT

Pyridoxine-pyrrolidone carboxylate (Metadoxine) for the treatment of metabolic dysfunction associated steatotic liver disease and increased alcohol intake (MetALD)

开始日期2025-04-15

申办/合作机构

Phramongkutklao College of Medicine

TCTR20250304001

/ Not yet recruiting临床2期IIT

Safety of high-level disinfection and sterilization for reprocessing the single-operator cholangioscopy in biliary tract disease

开始日期2025-03-10

申办/合作机构

Phramongkutklao College of Medicine

100 项与 Phramongkutklao College of Medicine 相关的临床结果

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0 项与 Phramongkutklao College of Medicine 相关的专利（医药）

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909

项与 Phramongkutklao College of Medicine 相关的文献（医药）

2025-12-31·Renal Failure

Critical appraisal of systematic reviews and meta-analyses: a step-by-step guide for nephrologists

Article

作者: Kaewput, Wisit ; Cheungpasitporn, Wisit ; Thongprayoon, Charat ; Tapolyai, Mihály ; Wathanavasin, Wannasit ; Fülöp, Tibor

BACKGROUNDSystematic reviews and meta-analyses play a pivotal role in evidence-based medicine, including nephrology, by consolidating findings from multiple studies. To maximize their utility, rigorous quality assessment during peer review is essential. Challenges such as heterogeneity, bias, and methodological flaws often undermine these studies, necessitating a structured appraisal process.METHODSThis guide outlines a framework for nephrologists on appraising systematic reviews and meta-analyses. Key areas include heterogeneity assessment using the I² statistic, interpretation of forest plots for pooled effect estimates, and the use of funnel plots with Egger's test to identify potential publication bias. Risk of bias is evaluated using RoB 2 for randomized controlled trials and ROBINS-I for non-randomized studies. Subgroup and sensitivity analyses, along with meta-regression, address heterogeneity and examine the robustness of findings.RESULTSThe I² statistic quantifies heterogeneity by estimating the proportion of variability in a meta-analysis. Funnel plots and Egger's test help detect publication bias. Major biases, such as selection, performance, detection, and publication bias, are identified using structured tools like AMSTAR 2, Cochrane RoB 2, and ROBINS-I. The GRADE framework further assesses the overall certainty of the evidence. Emphasis is placed on PRISMA compliance, protocol pre-registration, and transparent reporting of statistical analyses, subgroup, and sensitivity assessments. The inclusion of grey literature remains optional.CONCLUSIONBy focusing on key areas such as heterogeneity, risk of bias, and robust statistical methods, this guide enables nephrologists to critically appraise systematic reviews and meta-analyses, fostering better clinical decision-making and improved patient care in nephrology.

2025-12-31·Medical Education Online

Reliability and validity of simulation-based electrocardiogram assessment rubrics for cardiac life support skills among medical students using generalizability theory

Article

作者: Lertsakulbunlue, Sethapong ; Kantiwong, Anupong ; Thammasoon, Kaophiphat

Simulation-based learning (SBL) is effective for EKG interpretation training in the advanced cardiac life support (ACLS) context, enhancing motivation, confidence, and learning outcomes. However, research on the psychometrics of assessment rubrics for ACLS skills among pre-clinical students is limited. This study investigates the validity and reliability of assessment rubrics for ACLS skills, including EKG interpretation, scenario and pharmacological management, and teamwork. An SBL course that integrates basic EKG interpretation into ACLS Stations was conducted at Phramongkutklao College of Medicine, utilizing high-fidelity mannequins to simulate realistic scenarios, enrolling 96 medical students. The course consisted of five independent stations, and each student was assessed once by two raters using ten-item assessment rubrics. The rubrics included three domains: (1) EKG and ACLS algorithm skills, (2) management and mechanisms of action, and (3) affective domains. Validity evidence on the content was gathered, and construct validity was confirmed with confirmatory factor analysis (CFA). Inter-rater and internal consistency reliability were calculated. Generalizability theory was utilized to analyse the data. Three expert reviews yielded an item-objective congruence index of 0.67-1.00, with iterative validation through alpha and beta tests. The CFA demonstrated a good fit, but two questions with loading factors below 0.30 were removed, resulting in an eight-item assessment form. An inter-rater correlation of 0.70 (p < 0.001) and a Cronbach's alpha of 0.76 was demonstrated. To achieve a Phi-coefficient ≥0.80, three raters and at least 10 items are required in a p×i×r crossed design. With eight items, r:(p×i) nested design reliability was 0.69, 0.79, and 0.83 for one, two, and three raters, respectively. While a single rater with 10 items achieved a Phi-coefficient of 0.74. The rubrics for assessing ACLS skills among pre-clinical students demonstrated acceptable validity and reliability. A condensed eight-item rubric with acceptable reliability is proposed as a practical tool for optimizing assessment in future evaluations relevant to the pre-clinical context.

2025-06-01·Biochimie

Crystal structure of Leishmania orientalis triosephosphate isomerase at 1.88 Å resolution and its specific inhibitors

Article

作者: Choowongkomon, Kiattawee ; Pornthanakasem, Wichai ; Leelayoova, Saovanee ; Leartsakulpanich, Ubolsree ; Kuaprasert, Buabarn ; Kiriwan, Duangnapa ; Suginta, Wipa ; Mungthin, Mathirut ; Riangrungroj, Pinpunya

Leishmania orientalis, previously called L. siamensis, is a new species characterized as causing cutaneous leishmaniasis in Thailand. This study solves the crystal structure of the L. orientalis triosephosphate isomerase (LoTIM) in apo form at 1.88 Å resolution by using molecular replacement method. Tyrosine118 presents in the LoTIM protein sequence, whereas L. mexicana and Trypanosoma cruzi TIMs have a relative Cys118, which plays a major role in their specific ligand binding. Sulfur atom of the Cys57 thiol group is covalently bound to an arsenic (As) atom present in the precipitating solution. Although the electron density of loop-6 (Gly174-Tyr175-Gly176-Lys177-Val178) is missing in the structure due to this region lacking rigidity, the biological assembly of the two monomers of the LoTIM crystal structures are like that of L. mexicana and T. cruzi. 3D molecular protein-ligand docking was performed using the dimeric interfacial pocket of the enzyme as a ligand-binding receptor to identify its specific inhibitors. Five potential inhibiting compounds, including NSC639174, NSC606498, NSC110039, NSC58446, and NSC345647, were obtained with their IC₅₀ 2.79 ± 0.10, 3.28 ± 0.80, 3.67 ± 0.11, 4.59 ± 0.87 and 15.44 ± 0.14 μM, respectively. However, specific inhibition assays against TIMs from L. orientalis and rabbit muscle indicate that NSC639174 and NSC110039 are the most potent inhibitors for LoTIM, whereas NSC58446 inhibits well both the parasitic and rabbit enzymes.

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