作者: Xu, Dayuan ; Yao, Yuntao ; Zhang, Jie ; Wang, Xiaonan ; Lin, Qing ; Wang, Yue ; Huang, Zongqiang ; Chen, Wenfang ; Gong, Meiqiong ; Zhang, Chongyou ; Xian, Shuyuan ; Zhang, Wei ; Qian, Weijin ; Huang, Runzhi ; Lu, Bingnan ; Yin, Huabin ; Tong, Xirui ; Guo, Xinya ; Li, Yixu ; Chen, Yang ; Ji, Shizhao ; Lin, Min ; Gao, Yan ; Du, Erbin ; Qu, Xue ; Liu, Yifan ; Yang, Xinyue

The medical learning environment (MLE) exerted a profound influence on students' physical, psychological, and professional development. University prestige and ranking significantly shape students' self-perception and self-esteem. Nevertheless, the relationship between university category and MLE perception remained unexplored. Our study collected 10,901 questionnaires from 12 universities, spanning six categories. John Hopkins Learning Environment Scale (JHLES) was administrated to assess MLE perceptions. Utilizing a cross-sectional design, we categorized participants into high or low JHLES groups. Next, we conducted Chi-square and parametric or non-parametric tests to analyze the relationship between perception of MLE and university category. Subsequently, we performed a multivariate logistic regression and developed a nomogram to predict the likelihood of low JHLES scores. And the model performance was assessed through receiver operating characteristic (ROC) curves, decision curve analysis (DCA), and calibration curves. Our study revealed a significant association between university category and MLE perception. Specifically, students from top-tier universities were prone to negative MLE perceptions. Through multivariate logistic regression analysis, we validated university category as an influencing factor (p < 0.001). Moreover, our nomogram demonstrated reliability and consistency in predicting students' MLE perspectives. The university category emerged as a predictor for perception of MLE. Students from prestigious institutions manifested an increased likelihood of negative MLE perceptions.

2025-12-01·Rice

Endophytes Enhance Rice Inorganic Nitrogen Use Efficiency and Mitigate Nitrogen Loss Via Dissimilatory Nitrate Reduction To Ammonium in Paddy Soils

Article

作者: Xiao, Xiao ; An, Huanhuan ; Zhang, Zixian ; Yao, Jinzhi ; Qin, Shuping ; Liu, Mengting ; Luo, Xubiao ; Wei, Pangzhi ; Liu, Ting

Rice cultivation involves the large amounts of fertilizers application, but nitrogen (N) use efficiency remains low. Endophytes are considered key microorganisms that regulate nitrogen utilization and gaseous nitrogen loss in rice paddy ecosystems. However, systematic studies on the effectiveness and underlying mechanisms of endophytes in nitrogen utilization by crops within paddy fields are still scarce. This study employed microcosmic experiments to investigate the effects of endophytes on gaseous nitrogen loss from paddy soil and inorganic nitrogen utilization in rice plants. Results demonstrated that colonization of endophytes increased the efficiency of inorganic N use by approximately twofold. The simultaneous addition of rice roots colonized with endophytes to the soil resulted in a significant increase in ammonium (NH₄⁺) concentrations by 121-138% as well. Notably, colonization with endophytes reduced cumulative nitrous oxide (N₂O) emissions by 13-21% compared to the control. Importantly, the endophytes were shown to enhance soil redox capacity by increasing Clostridium abundance and Fe²⁺ concentration, thereby promoting the dissimilatory nitrate reduction to ammonium (DNRA) and mitigating soil N loss. These findings underline the potential of rice endophytes in paddy field management to enhance soil nitrogen retention and reduce nitrogen loss.

2025-12-01·WATER RESEARCH

Significant generational effects of tetracyclines upon the promoting plasmid-mediated conjugative transfer between typical wastewater bacteria and its mechanisms

Article

作者: Luo, Xubiao ; Wu, Ligui ; Zhang, Baoyin ; Zou, Xiaoming ; Mo, Yuanmin ; Yu, Jiayin ; Zhang, Hailin ; Rong, Lingling ; Yuan, Qing ; Chen, Hong ; Qiu, Hongmu

The generational effects of antibiotics are well-documented within the field of pharmacology, but their specific influence on the enhancement of plasmid-mediated conjugative transfer remains insufficiently understood, although plasmid-mediated conjugative transfer represents a crucial process for the horizontal dissemination of antibiotic resistance genes (ARGs). We pioneeringly investigate the effects of eight tetracyclines (TCs) across three generations on the conjugative transfer of RP4 plasmid, utilizing Escherichia coli strains isolated from typical wastewater. Results indicate that TCs enhance plasmid conjugative transfer, exhibiting significant generational effects. Average hormetic zone for conjugative transfer (ΔA^R) induced by 1st-generation TCs is 4.46 to 5.95 times greater than that induced by 2nd- and 3rd-generation TCs, respectively. Furthermore, this intriguing phenomenon is unrelated to TCs' degradation characteristics (P > 0.05), but is closely associated with bacterial biochemical responses (P < 0.05), including reactive oxygen species (ROS), cell membrane permeability, and ATP synthesis. Moreover, mechanistic results reveal that key structural parameters of TCs significantly impact their interactions with the essential protein UspD, which regulates biochemical processes and relevant genes on RP4 plasmid in donor bacteria, thereby substantially enhancing the plasmid conjugative transfer frequency and exhibiting significant generational effects (P < 0.001). Our findings shed light on the generational effects of TCs on plasmid conjugative transfer in wastewater treatment systems, offering theoretical supports for ARGs dissemination control.

项与井冈山大学相关的新闻（医药）

2024-10-08

·今日头条

Nature Communications | 张赫团队揭示长非编码RNA与编码基因协同调控肿瘤发生新机制

【导读】肿瘤细胞具有高度异质性，在同一或不同基因座上，常常存在异常长非编码RNA（long noncoding RNAs, lncRNAs）和异常编码基因协同调控肿瘤发生的现象【1-2】。然而，许多关键的lncRNA和编码基因源自不同的基因座，通过独特的协同作用和机制，导致肿瘤发生发展【3】。因此，探索这些来自同一或不同基因座的lncRNA和编码基因如何协同调控恶性肿瘤发生发展，具有重要科学意义和潜在临床价值。最新研究表明，这些异常lncRNA和编码基因受到从一维至三维层面上遗传和表观遗传因素的复杂转录调控。真核基因组在进化上高度保守，但其空间构象展现出细胞特异性和高度可塑性【4】。在这一背景下，染色质通过缠绕与折叠，形成了独特的三维基因组，其中基因座位点的开放或闭合状态直接关联着转录的活跃或沉默，从而为调控因子的精准结合创造了理想条件。然而，这种多层次调控的精确协同机制仍不完全清楚。 2024年9月27日，同济大学生命科学与技术学院张赫团队在 Nature Communications 上发表了题为“Prohibitin 2 orchestrates transcription of long noncoding RNA and coding gene to accelerate tumorigenesis”的最新研究论文，首次发现Prohibitin-2（PHB2）作为穿梭因子，调控了CANT2 lncRNA和编码基因CCBE1的异常协同转录，加速恶性黑色素瘤的发生发展，阐明了肿瘤表观遗传调控新模式。首先，该研究对黑色素瘤细胞进行ATAC-seq和RNA-seq分析，发现chr6p22.3位点处于开放染色质状态，并由此转录出一个新转录本，进一步分析转录本的编码能力和亚细胞定位后，明确其为核内lncRNA，并将其命名为CANT2。该研究发现黑色素瘤细胞中CANT2 lncRNA的启动子染色质可及性程度高，这为PHB2创造了理想结合环境，PHB2随后招募组蛋白甲基转移酶MLL2到CANT2启动子，增加H3K4三甲基化水平，激活致癌lncRNA CANT2的转录激活，显著促进肿瘤生长和转移。该研究接下来对CANT2 lncRNA敲除后的差异表达基因进行筛选，并结合分析ChIRP-MS数据，发现PHB2进一步与激活的CANT2转录本结合，定位到抑癌基因CCBE1的启动子区域。在此过程中，PHB2招募组蛋白去乙酰化酶HDAC1，降低CCBE1启动子区域的H3K27乙酰化水平，抑制CCBE1转录，显著促进黑色素瘤发生发展。当敲降PHB2后，PHB2与CANT2 lncRNA和编码基因CCBE1启动子的结合水平分别下降，两种组蛋白修饰因子MLL2和HDAC1结合也同步下降，显著改变CANT2和CCBE1启动子区组蛋白修饰水平，使CANT2和CCBE1的转录水平同时变化。此外，通过构建靶向CCBE1启动子的enChIP体系，该研究发现PHB2与CANT2均通过与CCBE1启动子直接结合来发挥作用。这些数据表明PHB2是新发现的具有协同调控lncRNA-基因转录的关键穿梭因子，为肿瘤潜在治疗提供了新的靶点选择。综上所述，该研究揭示了穿梭因子PHB2协同调控lncRNA和编码基因的新机制，为肿瘤发病机理提供了有趣的“一石二鸟”模型，同时PHB2这一特性也为未来发现更多类似疾病调控穿梭因子，并针对这类穿梭因子设计靶向药物提供了新路径。作为同济大学对口支援井冈山大学的联合学术成果，这是井冈山大学历史上首次以共同通讯单位在Nature系列子刊发表研究论文。同济大学丁天宜博士、博士研究生徐浩文和张晓宇博士是该文的共同第一作者，同济大学张赫教授为该文唯一通讯作者。同时，该工作还获得了同济大学生命科学与技术学院、同济大学附属东方医院、器官发育与表观遗传江西省重点实验室、井冈山大学附属医院临床医学研究中心、井冈山大学医学部和生命科学学院的大力支持，受到了国家重点研发计划、国家自然科学基金委和上海市科委等项目的资助。论文链接：https://www.nature.com/articles/s41467-024-52425-z 参考文献： 1. White NM, Maher CA. The potential use of lncRNAs found in the 8q24 region as biomarkers for colon cancer. Ann Oncol 28, 1688-1689 (2017). 2. Ahmadiyeh N, et al. 8q24 prostate, breast, and colon cancer risk loci show tissue-specific long-range interaction with MYC. Proc Natl Acad Sci U S A 107, 9742-9746 (2010). 3. Du M, et al. Remote modulation of lncRNA GCLET by risk variant at 16p13 underlying genetic susceptibility to gastric cancer. Sci Adv 6, eaay5525 (2020). 4. Finn EH, Misteli T. Molecular basis and biological function of variability in spatial genome organization. Science 365, (2019). 【关于投稿】转化医学网（360zhyx.com）是转化医学核心门户，旨在推动基础研究、临床诊疗和产业的发展，核心内容涵盖组学、检验、免疫、肿瘤、心血管、糖尿病等。如您有最新的研究内容发表，欢迎联系我们进行免费报道（公众号菜单栏-在线客服联系），我们的理念：内容创造价值，转化铸就未来！转化医学网（360zhyx.com）发布的文章旨在介绍前沿医学研究进展，不能作为治疗方案使用；如需获得健康指导，请至正规医院就诊。热门推荐活动点击免费报名 🕓 上海｜11月15日-16日 ▶ 2024第一届中国类器官转化医学大会点击对应文字查看详情

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