The objective of this paper was to evaluate the ability of a new panel of biomarkers to predict early renal injuries in rats with kidney injury induced by different drugs. SD rats were i.p. given gentamicin with 30, 100 and 200 mg·kg-1 once daily for 14 days, and urine and blood samples were collected at day 4, day 7, day 10 and day 15 before administration. SD rats were iv given doxorubicin with 3, 6 and9 mg·kg-1 once, and urine and blood samples were collected at day 4, day 7, day 10 and day 15. SD rats were ig given adenine with 30 and 60 mg·kg-1 once daily for 10 days, and urine and blood samples were collected at day 4, day 7 and day 11 before administration. Urine samples were collected and stored for kidney injury mol.-1(Kim-1), clusterin(Clu), cystatin C(Cys C) and β2-microglobulin(β2-MG) anal. by ELISA while the blood sample was used for Cre and BUN anal. The kidney was obtained for histol. examination after HE staining. Inflammatory cell infiltration, epithelial cells degeneration and necrosis, protein casts and tubular dilation were noted for animals treated with gentamycin for 10 days at 30 mg·kg-1, 7 days at 100 or 200 mg·kg-1. For animals treated at 30 mg·kg-1, Kim-1 and Cys C were significantly increased at day 4, β2-MG at day 7, while Cre and BUN showed no obvious change. For animals treated at 100 mg·kg-1, Clu, Cys C and β2-MG were significantly increased at day 4, BUN at day 10, while Cre showed no obvious change. For animals treated at 200 mg·kg-1, Kim-1, Clu, Cys C and β2-MG were significantly increased at day 4, while Cre and BUN at day 4 and day 7, resp. Tubular epithelial cell degeneration glass drops, protein casts and tubular dilation were noted for animals treated with doxorubicin for 15 days at 6 or 9 mg·kg-1. For animals treated at 6 mg·kg-1, Clu, Cys C and β2-MG were significantly increased at day 15, while Cre and BUN showed no obvious change. For animals treated at 9 mg·kg-1, β2-MG was significantly increased at day 10, Kim-1, Clu and Cys C at day 15, BUN was at day 15, and Cre showed no obvious change. Epithelial cells degeneration and tubular dilation were noted for animals treated with adenine for 11 days at 30 mg·kg-1 or 4 days at 60 mg·kg-1. For animals treated at 30 mg·kg-1, Kim-1 was significantly increased at day 4, Clu and Cys C at day 7, β2-MG at day 11, while Cre and BUN showed no obvious change. For animals treated at 60 mg·kg-1, Kim-1 was significantly increased at day 4, Clu and Cys C at day 7, β2-MG at day 11, while BUN and Cre at day 4. Pairwise comparison of ROC curves for biomarkers showed that the area under ROC curve of Kim-1 and Clu was significantly larger than that of BUN in rats treated with gentamicin. In the models induced by doxorubicin, AUC of Cys C and Clu was higher than that of Cre and BUN resp. β2-MG was of higher diagnostic value for early kidney injury induced by gentamicin and doxorubicin. Clusterin showed good ability to predict kidney injuries and could be used as biomarker for kidney injury.