People with chronic kidney disease (CKD) undergoing hemodialysis (HD) experience muscular complications, such as sarcopenia, which worsen their functional capacity and increase mortality. Exercise programs during HD are an effective strategy to combat sedentary behavior, and implementing them through innovative technologies could facilitate their adoption in clinics, benefiting more patients. Therefore, the study's objectives are to assess the feasibility and safety of a group exercise program supported by audiovisual media conducted during HD sessions. Additionally, it will analyze factors influencing adherence, identify potential causes for interruptions or failure to perform the prescribed exercise, and evaluate the risk and prevalence of sarcopenia, its association with low muscle strength, poor muscle quality, and physical inactivity.

开始日期2024-10-01

申办/合作机构

Universidade Federal de Juiz de Fora

[+1]

RBR-10rvpcp3 / Not yet recruitingN/AIIT

Acute effect of Osteopathic Manipulative Treatment on ankle range of motion and spatio-temporal and kinematic gait variables in individuals with limited dorsiflexion: pragmatic crossover randomized clinical trial

开始日期2024-10-01

申办/合作机构

Universidade Federal de Juiz de Fora

[+1]

100 项与 Universidade Federal de Juiz de Fora 相关的临床结果

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0 项与 Universidade Federal de Juiz de Fora 相关的专利（医药）

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19,371

项与 Universidade Federal de Juiz de Fora 相关的文献（医药）

2025-01-01·Journal of Ethnopharmacology

Lantana camara L. induces a multi-targeted cell death process in Leishmania amazonensis

Article

作者: Campos, Lara Melo ; Fabri, Rodrigo Luiz ; Antinarelli, Luciana Maria Ribeiro ; Midlej, Victor do Valle ; Bastos, João Pedro Reis Costa ; Silva Neto, Adolfo Firmino da ; Lemos, Ari Sérgio de Oliveira ; Machado, Patrícia de Almeida ; Coimbra, Elaine Soares ; Granato, Juliana da Trindade

ETNOPHARMACOLOGICAL RELEVANCELantana camara L. is a species known for its broad spectrum of bioactivities and is commonly used in folk therapy to address inflammatory, dermatological, gastrointestinal, intestinal worms and protozoan diseases. It boasts a diverse array of secondary metabolites such as terpenes, flavonoids, and saponins. However, despite its rich chemical profile, there remains a scarcity of studies investigating its antileishmanial properties.AIM OF THE STUDYThis research aims to explore the antileishmanial potential of L. camara, focusing also on its mechanism of action against Leishmania amazonensis.MATERIAL AND METHODSThe ethanolic extract of L. camara leaves (LCE) was obtained through static maceration, and its phytoconstituents were identified using UFLC-QTOF-MS. The colorimetric MTT method was conducted to determine the effect of LCE on promastigotes of L. amazonensis and murine macrophages. The anti-amastigote activity was evaluated by counting intracellular parasites in macrophages after Giemsa staining. Additionally, investigations into the mechanisms underlying its action were conducted using cellular and biochemical approaches.RESULTSLCE exhibited significant activity against both promastigotes and intracellular amastigotes of L. amazonensis, with IC₅₀ values of 12.20 μg/mL ± 0.12 and 7.09 μg/mL ± 1.24, respectively. These IC₅₀ values indicate very promising antileishmanial activity, comparable to those found for the positive control miltefosine (5.10 μg/mL ± 1.79 and 8.96 μg/mL ± 0.50, respectively). Notably, LCE exhibited negligible cytotoxicity on macrophages (IC₅₀ = 223.40 μg/mL ± 47.02), demonstrating selectivity towards host cells (SI = 31.50). The antileishmanial activity of LCE involved a multi-targeted cell death process, characterized by morphological and ultrastructural alterations observed through SEM and TEM analyses, as well as oxidative effects evidenced by the inhibition of trypanothione reductase, elevation of ROS and lipid levels, and mitochondrial dysfunction evaluated using DTNB, H₂DCFDA, Nile red, and JC-1 assays. Additionally, extraction of ergosterol and double labeling with annexin V and PI revealed modifications to the organization and permeability of the treated parasite's plasma membrane. LCE was found to consist predominantly of terpenes, with lantadenes A, B, and C being among the eleven compounds identified through UFLC-QTOF-MS analysis.CONCLUSIONSThe extract of L. camara presents a diverse array of chemical constituents, prominently featuring high terpene content, which may underlie its antileishmanial properties through a combination of apoptotic and non-apoptotic mechanisms of cell death induced by LCE. This study underscores the therapeutic potential of L. camara as a candidate for antileishmanial treatment, pending further validation.

2025-01-01·Talanta

Enhanced enzymatic electrochemical detection of an organophosphate Pesticide: Achieving Wide linearity and femtomolar detection via gold nanoparticles growth within polypyrrole films

Article

作者: Andrade, Gustavo F S ; Brolo, Alexandre G. ; Morawski, Rodrigo ; Santos, Jacqueline F L ; Griep, Jordana ; Demingos, Pedro G ; Demingos, Pedro G. ; Santos, Jacqueline F.L. ; Loguercio, Lara F. ; Loguercio, Lara F ; Andrade, Gustavo F.S. ; Brolo, Alexandre G

The indiscriminate use of pesticides in agriculture demands the development of devices capable of monitoring contaminations in food supplies, in the environment and biological fluids. Simplicity, easy handling, high sensitivities, and low limits-of-detection (LOD) and quantification are some of the required properties for these devices. In this work, we evaluated the effect of incorporating gold nanoparticles into indigo carmine-doped polypyrrole during the electropolymerization of films for use as an acetylcholinesterase (AChE) enzyme-based biosensor. As proof of concept, the pesticide methyl parathion was tested towards the inhibition of AChE. The enzyme was immobilized simply by drop-casting a solution, eliminating the need for any prior surface modification. The biosensors were characterized with cyclic voltammetry, scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy. The assays for the detection of methyl parathion with films containing polypyrrole, indigo carmine and AChE (PPy-IC-AChE) presented a sensitivity of 5.7 μA cm^-2 g^-1 mL and a LOD of 12 nmol L^-1 (3.0 ng L^-1) with a linear range from 1.3 x 10^-7 mol L^-1 to 1.0 x 10^-5 mol L^-1. The introduction of gold nanoparticles (AuNP) into the film (PPy-IC-AuNP-AChE) led to remarkable improvements on the overall performance, such as a lower redox potential for the enzymatic reaction, a 145 % increase in sensitivity (14 μA cm^-2 g^-1 mL), a wider detection dynamic range (from 1.3x10^-7 to 1.0x10^-3 mol L^-1), and a very low LOD of 24 fmol L^-1 (64 ag mL^-1). These findings underscore the potential of using AuNPs to improve the enzymatic performance of biosensor devices.

2025-01-01·Arquivos Brasileiros de Oftalmologia

Optic disc edema and visual loss from paracentral acute middle maculopathy mimicking optic neuritis

Article

作者: Benassi, Thais S A ; Monteiro, Mário L R ; Preti, Rony Carlos ; Souza, Eduardo C de ; Pereira, Clarissa R ; Mello, Luiz Guilherme M ; Cunha, Leonardo P ; Zacharias, Leandro C

Optic neuritis is an important cause of unilateral and acute visual loss in young adults, but other differential diagnoses should be considered, especially when the disease has an atypical presentation. This report presents the case of a young woman with reduced visual acuity in her right eye, associated with optic disc edema and a relative afferent pupillary defect, that was initially misdiagnosed as optic neuritis and subsequently found to have paracentral acute middle maculopathy, possibly secondary to subtle impending central retinal vein occlusion. This case emphasizes the need to remember that retinal vascular diseases can occasionally mimic optic neuritis. Detailed anamnesis and ophthalmic examination can avoid unnecessary interventions.

100 项与 Universidade Federal de Juiz de Fora 相关的药物交易

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100 项与 Universidade Federal de Juiz de Fora 相关的转化医学

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