Best production process of amikacin sulfate injection was discussed.Antioxidants, complexing agents, activated carbon adsorbent with different doses were chosen and under same process condition, clarity, color, pH, content and pyrogen were determinedThe optimized condition was antioxidant sodium metabisulfite of 0.20%, complexing agent sodium citrate of 0.10%, and adsorbent activated carbon of 0.03%.The process was stable and with good quality.