Oral squamous cell carcinoma (OSCC) is one of the top 10 most common malignancies worldwide. Oral squamous cell carcinoma forms a major health problem in many countries. The reason for the high incidence of oral cavity cancers in Pakistan, and South Asia in general, is the frequent, persistent, and prevalent use of substances classified as oral cancer risk factors. These include betel quid, areca nut, smoking, smokeless tobacco, viral infections such as EBV, HPV, poor oral hygiene (including sharp teeth and decay), ultraviolet (UV) exposure, nutrition, and genetic predisposition.
For several decades the management of OSCC consisted of surgery with or without radiotherapy or chemoradiotherapy. Associated with worse prognosis, overexpression of EGFR may lead to invasion, cellular migration, enhanced angiogenesis, decreased cell apoptosis, and increased metastatic potential. However, advances in immunotherapy have had a major impact on cancer treatment.
This study was exclusively designed to highlight the immunohistochemical detection of expression pattern of EGFR in already diagnosed cases of lip and OSCC in histopathology dept of AFIP and its correlation with the grade of the tumor. In this study, contact details of all diagnosed cases of SCC of lips and oral cavity from June to November 2023 were taken from the archives of Histopathology department. The patients were contacted for consent approval for participation. The data was collected (as per proforma attached), including demographic details, location of tumor. Blocks were retrieved, grade of tumor was identified followed by the application of EGFR marker. Patterns of expression of EGFR were evaluated and compared with grade of tumor to strengthen the correlation of this association.

开始日期2023-08-26

申办/合作机构

Combined Military Hospital Rawalpindi

[+1]

IRCT20240402061403N1

/ CompletedN/AIIT

Role of Dexmedetomidine in reduction of Post-Operative Emergence Delirium in children undergoing Adenotonsillectomy

开始日期2023-06-10

申办/合作机构

National University of Medical Sciences

100 项与 National University of Medical Sciences 相关的临床结果

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0 项与 National University of Medical Sciences 相关的专利（医药）

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1,053

项与 National University of Medical Sciences 相关的文献（医药）

2025-06-01·Current Microbiology

Bioactive Metabolites from Radioresistant Bacterium Kocuria sp. TMM 11 and Their Role in Prevention of Ultraviolet-Induced Photodamages

Article

作者: Alam, Tayyaba ; Abdullah, Muhammad ; Khan, Samiullah ; Badshah, Malik ; Farman, Muhammad ; Shah, Aamer Ali ; Ali, Mahwish ; Din, Salah Ud ; Hasan, Fariha

A radioresistant bacterium identified as Kocuria sp.TMM 11 was isolated from Thal desert. Kocuria sp.TMM11 demonstrated resistance (57.3% survival rate) to an ultraviolet B radiations dose of 4.1 × 10³ J/m², 61.5% survival rate to hydrogen peroxide (60 mM) and 57.1% survivability to mitomycin C (6 μg/mL). An orange carotenoid pigment from strain TMM 11 was extracted using solvent mixture of methanol, ethyl acetate and acetone (7:5:3), subsequently purified using C18 cartridge column. The purified fraction was analyzed by liquid chromatography mass spectrometry and compounds identified were rhodovibrin, phytoene, 4'-Hydroxy-4,4'-diaponeurosporene-4-oic acid and 3,4,3',4'-Tetrahydrospirilloxanthin. The purified fraction with mixture of carotenoid compounds, was evaluated for its antioxidant activity, total flavonoids, phenolic content and radio protective potential. These assessments were conducted in relation to its ability to prevent protein and lipids oxidation, as well as DNA strand breaks in vitro. The fraction showed strong antioxidant activity, as indicated by its ability to scavenge super oxides, with an IC50 value of 50.8 μg/mL. Additionally, it displayed 75.76% iron chelation activity. The purified fraction strongly inhibited oxidative damage to proteins and lipids, comparable to the activity of standard ascorbic acid. The total phenolic and flavonoid contents in extract were measured 11.6 and 9.8 μg in terms of gallic acid and quercetin equivalents per milligram of dried mass. Hence, it is concluded that the carotenoid mixture from Kocuria sp.TMM 11 not only inhibited DNA strands from UV mediated photo damages but also protected lipid and protein peroxidation and therefore could be a good candidate in radio protective drugs and as sunscreen.

2025-06-01·Food Chemistry: Molecular Sciences

Application of bacterioruberin from Arthrobacter sp. isolated from Xinjiang desert to extend the shelf-life of fruits during postharvest storage

Article

作者: Mohamad, Osama Abdalla Abdelshafy ; Abbasi, Sumra Wajid ; Bukhari, Sayed Muhammad Ata Ullah Shah ; Muhammad, Murad ; Liu, Yong-Hong ; Sajjad, Wasim ; Li, Wen-Jun

Post-harvest losses and rapid fruit ripening at room temperature are major challenges in preserving fruit quality. This study aimed to reduce such losses by applying a red carotenoid pigment, bacterioruberin extracted from an Arthrobacter sp. The carotenoid was characterized as bacterioruberin and its derivative tetra anhydrous bacterioruberin (λmax 490 nm), and an m/z value of 675 and 742 (M+ 1H)⁺¹. The annotated LIPID MAP demonstrated the presence of over 360 isoprenoids annotated transcripts. The compound exhibited significant antioxidant activity, with an IC₅₀ of 22 μg/mL, iron chelation and antibacterial activities indicating its potential as a natural preservative. When applied to grapes, peaches, and apricots, bacterioruberin (2 %) effectively prevented spoilage for six days at room temperature. Statistical analysis using one-way ANOVA revealed a significant correlation (p = 0.05) between treated and control groups in subjective quality attributes. Computational investigation with phospholipase D and VQ22 motif protein further supported the preservative potential of bacterioruberin.

2025-05-04·Mycologia

Genetic diversity of true morels ( Morchella ) in Pakistan inferred from multilocus DNA sequence data augmented by a retrospective analysis of Pakistani and Indian morel sequences in GenBank

Article

作者: Nisa, Surat Un ; Badshah, Hussain ; Shah, Sayed Afzal ; O’Donnell, Kerry ; Mumtaz, Abdul Samad

The current study was conducted to obtain the first detailed assessment of true morel (Morchella) species diversity in Pakistan. A collection of 179 morels was obtained by conducting field surveys in 13 districts during spring and autumn of 2014 through 2020. Species were identified phylogenetically by analyzing portions of two (RPB1, RPB2) or four (RPB1, RPB2, TEF1, ITS rDNA) marker loci together with authentic reference sequences of known species and by conducting BLASTn queries of National Center for Biotechnology Information (NCBI) GenBank. A retrospective analysis of morel sequences from Pakistani (N = 52) and Indian (N = 69) collections accessioned in GenBank was also conducted to expand and compare our knowledge of Morchella species diversity in both countries. Analyses of our 179-morel collection and the 52 Pakistani sequences deposited in GenBank indicated that at least 20 species are present in Pakistan, including five putatively novel taxa within the Elata subclade here informally distinguished as Morchella spp. (Mel-43 to Mel-47). Phylospecies Mel-45 and Mel-46 were described formally as M. pycnogranulata and M. waziristanica, respectively. By comparison, analyses of the 69 Indian sequences deposited in GenBank indicated that at least 12 species are extant in India. However, only 4/9 Pakistani and 3/12 Indian species were correctly identified in GenBank. Two collections of the steppe morel, M. steppicola (Mes-1), from Afghanistan were also included in the present study, which extends the southeasternmost range of this morphologically distinct species.

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