Suez Canal University

Cadmium (Cd) induces oxidative stress and inflammation, leading to hepatotoxicity in animals. Honokiol (HNK) has gained much attention owing to its anti-inflammatory and antioxidant properties, and may offer protection against liver diseases. However, whether HNK can improve Cd-induced ultrastructural and functional variation in hepatocytes is largely unknown. In this study, day-old broiler were divided into four treatment groups including control/untreated group Cd (50mg/L), HNK (50mg/kg), and Cd+HNK (50mg/L+50mg/kg) for 42 days, respectively. In Silico analysis was conducted to reveal the potential interaction of HNK with Bax and Bcl-2 proteins to determine how HNK affected these two protein targets to mediate the effects of Cd toxicity. Results revealed that Cd exposure caused significant damage to the ultrastructure and functional activity of hepatocytes compared to the control group. Notably, HNK treatment helped recover and maintain the integrity of the nucleus and mitochondrial cristae in hepatocytes. In addition, HNK reduced oxidative stress with the increased activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and decreased malondialdehyde (MDA) content in the liver tissue. The HNK prevented lipid accumulation in the liver tissue induced by cadmium toxicity. Furthermore, HNK decreased the expression of apoptotic protein and gene expression of Caspase-3, and increased expression of the anti-apoptotic protein Bcl-2 by immunohistochemistry and qPCR. In conclusion, findings of the present study revealed the potential of HNK to alleviate Cd-induced ultrastructural and functional perturbations that cause hepatotoxicity in chicken, making it a promising therapeutic agent for Cd poisoning in animals.

The impact of maternal diabetes on the fetal heart is complex and not fully understood. The study aimed to investigate how different types and stages of maternal diabetes affect fetal heart function and ventricular remodeling during prenatal and postnatal periods. The study included 45 pregnant women with diabetes and 15 with uncomplicated pregnancies. Fetal echocardiograms were performed between 28 and 32 weeks of gestation using different techniques to assess cardiac function. Measurements of ventricular transverse and longitudinal diameters, as well as the sphericity index, were determined. Postnatal follow-up echocardiograms were also performed. The diabetic pregnant group was divided into three subgroups: 15 with GDM and 30 with PGD, including 15 wellcontrolled and 15 uncontrolled cases. The E and E/A ratio values for the mitral and tricuspid valves were significantly lower in fetuses and neonates affected by diabetes compared to the healthy group. The Tei-Doppler index for the left ventricle was significantly higher in pregnancies affected by diabetes, affecting both fetuses and neonates. The right ventricle exhibited significant differences in all groups of diabetic pregnancies in fetuses and postnatally in cases of pregestational diabetes. The sphericity index differed significantly in all fetal diabetic groups and in neonates compared to controls, except for the LV in the GDM cohort. Postnatally, the sphericity index of the RV increased compared to fetal values, while the LV sphericity index remained unchanged. Maternal diabetes mellitus affects fetal cardiac function and structure, which may continue into the early days of life.

Patient safety (PS) is a cornerstone of nursing education, yet evidence on how PS competencies relate to evidence-based practice (EBP) among nursing students remains limited. This study examined PS competencies and their association with EBP across 3 Middle Eastern countries.

To assess levels of PS competencies among undergraduate nursing students, compare results across demographic and academic groups, and evaluate EBP as a predictor of PS.

A cross-sectional survey was conducted with 615 undergraduate nursing students from 4 universities in Oman, Jordan, and Egypt. Participants completed validated tools measuring PS and EBP competencies.

Overall PS competence was moderate (M = 152.1, SD = 28.1). Among subscales, skills scored the highest, followed by attitudes, while knowledge was lowest. EBP competencies were also moderate and significantly correlated with PS ( r = .59, P < .001). Regression analysis showed that EBP was the strongest predictor of PS ( B = 0.71, P < .001), explaining 34.8% of the variance in scores.

Nursing students across 3 countries demonstrated moderate PS and EBP competencies, with notable gaps in safety knowledge. The strong predictive role of EBP highlights the need to integrate both domains into curricula to better prepare students for safe, evidence-based practice.