Sorghum (Sorghum bicolor L.) production in Ethiopia is often affected by a range of abiotic factors, mainly drought.This study aimed to evaluate the genetic variation and heritability of shoot and root traits that contribute to drought tolerance in sorghum genotypes.A total of 158 genotypes were analyzed using a high-throughput phenotyping platform.Highly significant (p < .001) variations were observed for all traits considered, with strong and pos. correlations among root-related traits at p < .001 (r = 0.76-0.99) and shoot traits at p < .001 (r = 0.27-0.98).The observed range of the root angles varied from 7.75° to 25.17°, with an average of 14.81°.The heritability estimates ranged from 35.51% to 76.25%, with an average of 62.40%.At an eigenvalue of greater than 1, four principal components explained 87.20% of the variability of the data sets, with PC1 and PC2 accounting for 38.10% and 34.10% of the total variability.Cluster anal. grouped the genotypes into four genetic groups.We believe that this study provides valuable insights into the genetic diversity and heritability of root and shoot-related traits in sorghum.In addition, these findings can assist in decision-making regarding the prioritization of sorghum genotypes in breeding programs targeting drought.