Delving into the Latest Updates on National Institute of Chemistry Slovenia with Synapse

概览

疾病领域	数量

感染	1

排名前五的药物类型	数量

小分子化药	1

排名前五的靶点	数量

TOP	1

关联

1

项与 National Institute of Chemistry Slovenia 相关的药物

Compound 12(NCI)

靶点

TOP

作用机制

TOP 抑制剂

在研机构

National Institute of Chemistry Slovenia [+1]

原研机构

University of Ljubljana [+1]

在研适应症

耐甲氧西林金黄色葡萄球菌感染

非在研适应症-

最高研发阶段临床前

首次获批国家/地区-

首次获批日期-

1

项与 National Institute of Chemistry Slovenia 相关的临床试验

NCT04679428 / Unknown statusN/AIIT

Immunological Characteristics of COVID-19 Patients and Determination of Neutralizing Antibodies Against SARS-CoV-2 Virus

The study will look at the immunological characteristics of COVID-19 patients and determine neutralizing antibodies against SARS-CoV-2 virus.

开始日期2020-05-07

申办/合作机构

National Institute of Chemistry Slovenia

100 项与 National Institute of Chemistry Slovenia 相关的临床结果

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0 项与 National Institute of Chemistry Slovenia 相关的专利（医药）

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1,245

项与 National Institute of Chemistry Slovenia 相关的文献（医药）

2024-12-01·Chemical Engineering Communications

Effect of 662 keV Cs ¹⁺ ion radiation on the dielectric behavior of the PVDF/Ba _0.5 Sr _0.5 TiO ₃ thick films

作者: Kaur, Shobhneek ; Gupta, Aayush ; Jaidka, Sachin ; Sabharwal, Arvind D. ; Likozar, Blaz

2024-11-01·Nature Chemical Biology

Massively parallel measurement of protein–protein interactions by sequencing using MP3-seq

Article

作者: Michel, Cirstyn ; Ljubetič, Ajasja ; Baker, David ; Baryshev, Alexandr ; La Fleur, Alyssa ; Seelig, Georg ; Groves, Benjamin

Protein-protein interactions (PPIs) regulate many cellular processes and engineered PPIs have cell and gene therapy applications. Here, we introduce massively parallel PPI measurement by sequencing (MP3-seq), an easy-to-use and highly scalable yeast two-hybrid approach for measuring PPIs. In MP3-seq, DNA barcodes are associated with specific protein pairs and barcode enrichment can be read by sequencing to provide a direct measure of interaction strength. We show that MP3-seq is highly quantitative and scales to over 100,000 interactions. We apply MP3-seq to characterize interactions between families of rationally designed heterodimers and to investigate elements conferring specificity to coiled-coil interactions. Lastly, we predict coiled heterodimer structures using AlphaFold-Multimer (AF-M) and train linear models on physics-based energy terms to predict MP3-seq values. We find that AF-M-based models could be valuable for prescreening interactions but experimentally measuring interactions remains necessary to rank their strengths quantitatively.

2024-10-02·Biochemical Journal

Entangling roles of cholesterol-dependent interaction and cholesterol-mediated lipid phase heterogeneity in regulating listeriolysin O pore-formation

Article

作者: Chattopadhyay, Kausik ; Anderluh, Gregor ; Lata, Kusum

Cholesterol-dependent cytolysins (CDCs) are the distinct class of β-barrel pore-forming toxins (β-PFTs) that attack eukaryotic cell membranes, and form large, oligomeric, transmembrane β-barrel pores. Listeriolysin O (LLO) is a prominent member in the CDC family. As documented for the other CDCs, membrane cholesterol is essential for the pore-forming functionality of LLO. However, it remains obscure how exactly cholesterol facilitates its pore formation. Here, we show that cholesterol promotes both membrane-binding and oligomerization of LLO. We demonstrate cholesterol not only facilitates membrane-binding, it also enhances the saturation threshold of LLO-membrane association, and alteration of the cholesterol-recognition motif in the LLO mutant (LLOT515G-L516G) compromises its pore-forming efficacy. Interestingly, such defect of LLOT515G-L516G could be rescued in the presence of higher membrane cholesterol levels, suggesting cholesterol can augment the pore-forming efficacy of LLO even in the absence of a direct toxin-cholesterol interaction. Furthermore, we find the membrane-binding and pore-forming abilities of LLOT515G-L516G, but not those of LLO, correlate with the cholesterol-dependent rigidity/ordering of the membrane lipid bilayer. Our data further suggest that the line tension derived from the lipid phase heterogeneity of the cholesterol-containing membranes could play a pivotal role in LLO function, particularly in the absence of cholesterol binding. Therefore, in addition to its receptor-like role, we conclude cholesterol can further facilitate the pore-forming, membrane-damaging functionality of LLO by asserting the optimal physicochemical environment in membranes. To the best of our knowledge, this aspect of the cholesterol-mediated regulation of the CDC mode of action has not been appreciated thus far.

100 项与 National Institute of Chemistry Slovenia 相关的药物交易

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100 项与 National Institute of Chemistry Slovenia 相关的转化医学

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