Objective:To establish and evaluate quant. detection method for IL-6 based on magnetic microparticale chemilumineseent immunoassay(CLIA).Methods:The anal. system was composed of magnetic microparticale coated with anti-human IL-6 antibody, serum and anti-human IL-6 antibody labeled with horseradish peroxidase. Based on the CLIA platform, the double antibody sandwich mode was adopted to test quant. of IL-6 antigen. Moreover, the reaction conditions of IL-6 were optimized and the anal. performance was evaluated.Results:It was indicated that the limit of blank(LoB),limit of detection(LoD) and functional sensitivity(FS) were 0.5,2 and 2.5 pg/mL.The precision of the within variantion and interanal. variantion and total imprecision were lower than 7%,10%,12%,resp.The linear range was within the range between 3 and 1 000 pg/mL,and the maximum dilution limit was 1:7.The recovery was within(100±15)%.The results measured by adding lower than 200 mg/dL Hb,2 000 mg/dL triglyceride and 45 mg/dL bilirubin to human sera with high and low concentrations of IL-6 were undisturbed.The clin. serum samples were tested simultaneously by this method and Roche system, and the curve regression equation of the results was Y=0.955 X+9.263,R∼2=0.993 2. Conclusion:The quant. detection method for IL-6 introduced in this study was demonstrated to have effective performance and can meet the need of clin. requirement.