Objective To establish a HPLC method for determining the content of adenosine in compound Weining tablets.Methods HPLC was used to determine the content of adenosine with C18 column (4.6 mm × 250 mm, 5μm) and methanol-0.02 mol/L disodium hydrogen phosphate solution (20:80, V/V) as the mobile phase.The flow rate was 1.0 mL/min.Results The calibration curve was linear in the range of 0.8304-8.3040μg/mL for adenosine.The average recovery was 97.86%, and RSD was 2.6% (n = 9).Conclusion This method is precise, convenient and rapid, and can be used for the quality control of adenosine in compound Weining tablets.