更新于：2024-11-01

University Hospital Hradec Kralove

Czechia

1,000-5,000

Czechia

1,000-5,000

更新于：2024-11-01

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项与 University Hospital Hradec Kralove 相关的临床试验

NCT03644641 / Not yet recruitingN/AIIT

The Influence of Prone Position for Spinal Surgery on Visual Acuity, a Comparison of Volatile Anesthesia With Desflurane to Total Intravenous Anesthesia With Propofol

This study investigated the effect of desflurane and propofol anesthesia on visual acuity in patients in prone position for spinal surgery. Many trials have investigated the effects of different anesthetic agents on intraocular pressure, propofol may reduce intraocular pressure more than other intravenous anesthetics.

开始日期2025-11-28

申办/合作机构

University Hospital Hradec Kralove

NCT06252454 / Recruiting临床2/3期IIT

The Impact of Inhaled Furosemide and Perorally Administered Levodropropizine on Dyspnea in Patients With Respiratory Diseases

The goal of this monocentric double blinded cross over trial is to evaluate safety and tolerability of inhaled furosemide in patients with pulmonary diseases and dyspnea.
The main question[s] it aims to answer are:
is inhaled furosemide safe and well tolerated?
is inhaled furosemide capable of dyspnea suppression and is there any additive effect of levodropropizine
Participants will be given:
inhaled furosemide or placebo in double blinded cross over manner. Dyspnea severity and vital functions will be measured, adverse reactions monitored
all participants will receive levodropropizine open label. Dyspnea severity and vital functions will be measured, adverse reactions monitored

开始日期2024-02-27

申办/合作机构

University Hospital Hradec Kralove

[+1]

NCT06431945 / RecruitingN/AIIT

Early Detection of Hepatitis C in Injection Drug Users

The project is a national, prospective, multicenter, non-interventional pilot project of screening HCV in PWID in the Czech Republic.
The main goal of the project is to methodically prepare, implement and evaluate a pilot project that will verify the suitability of the proposed procedure of early detection of Hepatitis C and setting up and testing new methods and implementation into the system of social health care.

开始日期2023-12-15

申办/合作机构

Institute of Clinical and Experimental Medicine

[+1]

100 项与 University Hospital Hradec Kralove 相关的临床结果

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0 项与 University Hospital Hradec Kralove 相关的专利（医药）

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1,282

项与 University Hospital Hradec Kralove 相关的文献（医药）

2024-12-31·Autophagy Reports

Autophagy Determines Distinct Cell Fates in Human Amnion and Chorion Cells

Article

作者: Mysorekar, Indira U ; Khanipov, Kamil ; Severino, Mary Elise L ; Radnaa, Enkhtuya ; Dalmacio, Leslie Michelle M ; Menon, Ramkumar ; Kacerovsky, Marian ; Richardson, Lauren ; Kammala, Ananth Kumar

Human fetal membranes (amniochorion) that line the intrauterine cavity consist of two distinct cell layers; single-layer amnion epithelial cells (AEC) and multilayer chorion trophoblast cells (CTC). These layers are connected through a collagen-rich extracellular matrix. Cellular remodeling helps support membrane growth and integrity during gestation and helps to maintain pregnancy. Preterm prelabor rupture of the human amniochorionic (fetal) membrane (pPROM) is antecedent to 40% of all spontaneous preterm birth. Oxidative stress (OS) induced activation of the p38 MAPK due to various maternal risk exposures and the amniochorion cells' senescence are reported pathological features of pPROM. Our transcriptomics analysis implicated dysregulated autophagy and epithelial-mesenchymal transition (EMT) in fetal membranes from pPROM. The molecular interplay between OS-induced p38 MAPK activation, autophagy, and EMT was investigated in AECs and CTCs to better understand the involvement of autophagy and EMT. We report the differential impact of OS on the autophagic machinery in AECs and CTCs, resulting in distinct cell fates. In AECs, OS-induced p38 MAPK activation causes autophagosome accumulation and reduced autophagic flux mediated by decreased ULK1 activity and kinase activity, leading to senescence. In CTCs, induction of autophagy has a limited effect; however, inhibition of autophagy led to SQSTM1-mediated EMT of trophoblast cells. Autophagy, EMT, and senescence were associated with proinflammatory changes. Thus, AECs and CTCs respond differently to OS via differential autophagy response, partly mediated via p38 MAPK. Besides senescence, OS-induced autophagy dysregulation in amniochorion cells may play a mechanistic role in pPROM pathophysiology.

2024-12-31·Journal of Obstetrics and Gynaecology

Absolute counts of leukocyte subsets in peripheral blood in pregnancies complicated by preterm prelabour rupture of membranes

Article

作者: Stranik, Jaroslav ; Kukla, Rudolf ; Kacerovska Musilova, Ivana ; Kacerovsky, Marian ; Soucek, Ondrej ; Andrys, Ctirad ; Bolehovska, Radka

BACKGROUNDThis study aimed to assess variations in the absolute counts of various leukocyte subsets in the peripheral blood of women with pregnancies affected by preterm prelabour rupture of membranes (PPROM), in relation to the presence of intra-amniotic inflammation (IAI).METHODSThe study included fifty-two women with singleton pregnancies experiencing PPROM. Absolute counts of different leukocyte subpopulations, such as granulocytes, monocytes, lymphocytes, T cells and their subsets, B cells and their subsets, and NK cells and their subsets, were measured in maternal peripheral blood samples using multicolour flow cytometry. IAI was identified by elevated concentrations of interleukin 6 (IL-6) in the amniotic fluid, which was collected through transabdominal amniocentesis.RESULTSWomen with IAI exhibited higher absolute counts of leukocytes (p = 0.003), granulocytes (p = 0.008), and monocytes (p = 0.009). However, the presence of IAI did not significantly affect the absolute counts of lymphocytes or their subpopulations.CONCLUSIONSThe study found that IAI is associated with changes in the absolute counts of leukocytes from the innate immunity compartment in the peripheral blood of women with pregnancies complicated by PPROM. Conversely, it does not significantly alter the counts of cells from the adaptive immune system. The changes observed may reflect the natural, temporal, and localised characteristics of IAI.

2024-12-31·Platelets

Correlations among different platelet aggregation pathways in a group of healthy volunteers

Article

作者: Javorská, Lenka ; Hrubša, Marcel ; Fadraersada, Jaka ; Skořepa, Pavel ; Paclíková, Markéta ; Matoušová, Kateřina ; Carazo, Alejandro ; Musil, František ; Mladenka, Přemysl ; Konečný, Lukáš ; Karlíčková, Jana ; Blaha, Vladimír ; Šmahelová, Alena ; Gunaseelan, Catherine ; Kujovská Krčmová, Lenka

Platelet aggregation is a complicated process mediated by different signaling pathways. As the process is highly complex and apparently redundant, the relationships between these pathways are not yet fully known. The aim of this project was to study the interconnections among seven different aggregation pathways in a group of 53 generally healthy volunteers aged 20 to 66 years. Platelet aggregation was induced with thrombin receptor activating peptide 6 (TRAP), arachidonic acid (AA), platelet activating factor 16 (PAF), ADP, collagen, thromboxane A₂ analogue U46619 or ristocetin (platelet agglutination) ex vivo in fasting blood samples according to standardized timetable protocol. Additionally, some samples were pre-treated with known clinically used antiplatelet drugs (vorapaxar, ticagrelor or acetylsalicylic acid (ASA)). Significant correlations among all used inducers were detected (Pearson correlation coefficients (r_P): 0.3 to 0.85). Of all the triggers, AA showed to be the best predictor of the response to other inducers with r_P ranging from 0.66 to 0.85. Interestingly, the antiplatelet response to ticagrelor strongly predicted the response to unrelated drug vorapaxar (r_P = 0.71). Our results indicate that a response to one inducer can predict the response for other triggers or even to an antiplatelet drug. These data are useful for future testing but should be also confirmed in patients.

100 项与 University Hospital Hradec Kralove 相关的药物交易

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100 项与 University Hospital Hradec Kralove 相关的转化医学

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