The purpose of this study is to determine if measurements of active collagen deposition using [68Ga]CBP8 positron emission tomography (PET) and tissue injury using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) can predict an individual patient's pace of disease progression in non-idiopathic pulmonary fibrosis interstitial lung disease (non-IPF ILD) and identify which individuals will develop progressive pulmonary fibrosis.

开始日期2024-09-01

申办/合作机构

The Massachusetts General Hospital

NCT05619302 / Recruiting临床3期IIT

Molecular Imaging of Myocardial Fibrosis in Cardiac Amyloidosis

The primary aim of our pilot study is to determine whether fibrosis in the heart can be measured with [68Ga]CBP8, a positron emission tomography (PET) probe, using PET/magnetic resonance imaging (MRI) imaging, in 30 individuals with documented cardiac amyloidosis. The investigators will also enroll 15 individuals with recent myocardial infarction and 15 individuals with hypertrophic cardiomyopathy as positive controls for fibrosis, and the investigators will enroll 5 individuals without cardiovascular disease to undergo [68Ga]CBP8 PET/MRI imaging as a healthy control group.
The primary hypothesis of this study is that [68Ga]CBP8 will bind to interstitial collagen and quantify myocardial fibrosis in patients with cardiac amyloidosis. The investigators hypothesize that [68Ga]CBP8 uptake will be greater in patients with cardiac amyloidosis, myocardial fibrosis, and hypertrophic cardiomyopathy than in healthy controls. Secondly, the investigators also hypothesize that [68Ga]CBP8 activity more strongly correlates with standard MRI measures in patients with recent myocardial infarction and hypertrophic cardiomyopathy (where extracellular expansion is caused by myocardial fibrosis/collagen deposition) than in patients with cardiac amyloidosis (where myocardial fibrosis is combined with infiltration).

开始日期2023-01-03

申办/合作机构

The Brigham & Women's Hospital, Inc.

NCT05417776 / Recruiting临床2期IIT

Collagen-targeted PET Imaging for Early Interstitial Lung Disease

The goal of this study is to investigate the ability of [68Ga]CBP8 to detect collagen deposition in early interstitial lung disease.

开始日期2022-09-28

申办/合作机构

The General Hospital Corp.

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项与胶原蛋白I 相关的文献（医药）

2025-01-01·Biomaterials Advances

3D-printed surfaces of titanium implant: the fibroblasts response

Article

作者: Gregory, Shaun D ; Zeng, Lingxiao ; Zhu, Yuman ; Armstrong, Sophie ; Dyson, Jennifer M ; Gregory, Shaun D. ; Huang, Aijun ; Dyson, Jennifer M.

Ti-6Al-4V (wt%) is the most widely used titanium alloy and its additive manufactured (or 3D printed) parts with near net-shape have provided great advantages for biomedical applications. While the impact of surface roughness on the biocompatibility of 3D-printed Ti-6Al-4V part is recognized, further exploration is needed to fully understand this complex relationship. Hence, this study presents a comprehensive evaluation of as-printed Ti-6Al-4V structures, both with and without surface texturing, with particular focus on the fibroblast response. Alongside a flat surface, or as-printed surface, two different types of surface textures: diamond texture and diamond crystal texture, were meticulously designed and printed through laser powder bed fusion (LPBF). The viability, cell adhesion, and morphology of human and murine fibroblasts seeded on the surface patterns was investigated, as well as the distribution of extracellular matrix (ECM) proteins (collagen I, fibronectin). The results demonstrated that the as-fabricated surface morphologies did not impact fibroblast viability, however, a reduced density of human fibroblasts was observed on the diamond texture surface, likely owing to the upright strut structure preventing cell adhesion. Interestingly, spreading of the human, but not murine, fibroblasts was limited by the remaining partially-sintered powders. The relative intensity of ECM protein signals was unaffected, however, ECM protein distribution across the surfaces was also altered. Thus, the as-printed substrates, particularly with diamond crystal struts, present a promising avenue for the cost-effective and efficient fabrication of Ti-6Al-4V components for medical applications in the future.

2024-12-31·Scandinavian Cardiovascular Journal

Serum microRNA-125b-5p expression in patients with dilated cardiomyopathy combined with heart failure and its effect on myocardial fibrosis

Article

作者: Zhang, Yingjie ; Xiang, Yi ; Wu, Jiaru ; Huang, Quan ; Ou, Boqing ; Deng, Daqing

OBJECTIVEThis paper was performed to decipher the serum microRNA (miR)-125b-5p expression in patients with dilated cardiomyopathy (DCM) combined with heart failure (HF) and its effect on myocardial fibrosis.METHODSSerum miR-125b-5p expression, LVEDD, LVESD, LVEF, LVFS, and NT-proBNP levels were evaluated in clinical samples. A rat DCM model was established by continuous intraperitoneal injection of adriamycin and treated with miR-125b-5p agomir and its negative control. Cardiac function, serum TNF-α, hs-CRP, and NT-proBNP levels, pathological changes in myocardial tissues, cardiomyocyte apoptosis, and the expression levels of miR-125b-5p and fibrosis-related factors were detected in rats.RESULTSIn comparison to the control group, the case group had higher levels of LVEDD, LVESD, and NT-pro-BNP, and lower levels of LVEF, LVFS, and miR-125b-5p expression levels. Overexpression of miR-125b-5p effectively led to the improvement of cardiomyocyte hypertrophy and collagen arrangement disorder in DCM rats, the reduction of blue-stained collagen fibers in the interstitial myocardium, the reduction of the levels of TNF-α, hs-CRP, and NT-proBNP and the expression levels of TGF-1β, Collagen I, and α-SMA, and the reduction of the number of apoptosis in cardiomyocytes.CONCLUSIONOverexpression of miR-125b-5p is effective in ameliorating myocardial fibrosis.

2024-12-31·Annals of Medicine

M2 macrophages promote subconjunctival fibrosis through YAP/TAZ signalling

Article

作者: Li, Jingguo ; Cui, Haohao ; Li, Chengcheng ; Guo, Zhihua ; Cheng, Boyuan ; Geng, Xingchen ; Chu, Dandan ; Li, Zhanrong ; Wang, Yiwei ; Liu, Ruixing

PURPOSETo evaluate the role of M2 macrophages in subconjunctival fibrosis after silicone implantation (SI) and investigate the underlying mechanisms.MATERIALS AND METHODSA model of subconjunctival fibrosis was established by SI surgery in rabbit eyes. M2 distribution and collagen deposition were evaluated by histopathology. The effects of M2 cells on the migration (using wound-scratch assay) and activation (by immunofluorescence and western blotting) of human Tenon's fibroblasts (HTFs) were investigated.RESULTSThere were more M2 macrophages (CD68+/CD206+ cells) occurring in tissue samples around silicone implant at 2 weeks postoperatively. Dense collagen deposition was observed at 8 weeks after SI. In vitro experiment showed M2 expressed high level of CD206 and transforming growth factor-β1 (TGF-β1). The M2-conditioned medium promoted HTFs migration and the synthesis of collagen I and fibronectin. Meanwhile, M2-conditioned medium increased the protein levels of TGF-β1, TGF-βR II, p-Smad2/3, yes-associated protein (YAP), and transcriptional coactivator with PDZ-binding motif (TAZ). Verteporfin, a YAP inhibitor, suppressedTGF-β1/Smad2/3-YAP/TAZ pathway and attenuated M2-induced extracellular matrix deposition by HTFs.CONCLUSIONSTGF-β1/Smad2/3-YAP/TAZ signalling may be involved in M2-induced fibrotic activities in HTFs. M2 plays a key role in promoting subconjunctival fibrosis and can serve as an attractive target for anti-fibrotic therapeutics.

项与胶原蛋白I 相关的新闻（医药）

2024-06-26

·生物谷

Adv Mater | 西班牙研究团队成功制造微型肾脏，模拟复杂血管结构，开辟类器官研究新篇章

在一项新的研究中，来自西班牙加泰罗尼亚生物工程研究所的研究人员介绍了一种在实验室中制造微型肾脏（mini-kidney）的新方法，这项技术成功复制了人类肾脏的复杂血管结构，并成功将三维肾脏类器官（kidney organoids）与内皮类器官（endothelial organoids）相结合，推进了类器官研究的边界。相关研究结果近期发表在Advanced Materials期刊上，论文标题为“Natural Hydrogels Support Kidney Organoid Generation and Promote In Vitro Angiogenesis”。类器官作为模拟真实器官功能的三维体外模型，在疾病模型构建和药物筛选中至关重要，尽管如此，它们在复现如神经系统、免疫系统及血管系统的复杂性方面历来存在局限。十多年来，在实验室中再现肾脏这一高度灌注组织的复杂血管系统一直是科学家们面临的挑战。论文共同通讯作者、加泰罗尼亚生物工程研究所首席研究员Núria Montserrat Pulido 解释说，这项新的研究描述了一种从未尝试过的方法：“我们开创性地将肾类器官与血管类器官整合，这要求精确掌握两者发育的时间节点，确保在最理想的时机进行组合。”她补充道，“两类器官一旦结合，便会迅速启动交互作用，仅三小时，就展现出惊人的协同效应。” 此研究成果不仅深化了对肾类器官发展机制的理解，也铺垫了促进类器官内部血管系统形成的崭新路径，有望在类器官移植和涉及血管功能障碍疾病的临床研究中发挥关键作用。图片来自Advanced Materials, 2024, doi:10.1002/adma.202400306 论文共同通讯作者兼论文第一作者、加泰罗尼亚生物工程研究所Montserrat团队研究员Elena Garreta Bahima说，“对于那些通过血液循环影响肾脏的全身性疾病，比如自身免疫病和糖尿病，我们的类器官模型需要具备相应的血管系统。我们认为，在这项研究中使用的方法也可能应用于其他得类器官模型。” Montserrat说，下一步研究团队计划在微流控平台上集成这些血管化的类器官，以进行更高级别的疾病模拟实验，并探索与心脏类器官等其他类器官的血管连通。在之前的研究中，研究人员已发现他们构建的肾类器官含有血管前体细胞——内皮细胞，即最终形成血管的细胞。基于此，他们利用猪或人的肾组织，制造出一种可用作三维打印墨水的生物相容性水凝胶。在本研究中，研究人员进一步优化了技术，使其更方便、更经济。在构成水凝胶的所有蛋白中，他们选择了肾组织胞外基质中含量最高的蛋白之一：胶原蛋白I（collagen I），并生成了一种生物相容性凝胶，来验证他们的实验结果。这项新的研究是一个重要里程碑，标志着在应用尖端科技应对复杂健康问题的先进治疗领域取得了重大进展。参考资料： Elena Garreta et al. Natural Hydrogels Support Kidney Organoid Generation and Promote In Vitro Angiogenesis. Advanced Materials, 2024, doi:10.1002/adma.202400306. 本文仅用于学术分享，转载请注明出处。若有侵权，请联系微信：bioonSir 删除或修改！

抗体药物偶联物

2023-05-09

·PRNewswire

Study Shows Preventive Effects and Protective Potential of Astaxanthin on Collagen Damage

BRANCHBURG, N.J., May 9, 2023 /PRNewswire/ -- A study recently published in the Journal of Food & Nutritional Sciences, found that Lycored's astaxanthin prevented against collagen damage from neutrophils, discovering its potential to protect collagen and enhance skin health. This study assessed the addition of astaxanthin to neutrophils, the key players in the skin's immune response and photoaging, and the combined effects on collagen presence. The two most prevalent types of collagen are I and III, which play major roles in skin elasticity. When skin is exposed to damaging elements it triggers an immune response in the skin that signals neutrophils, which try to eliminate the harmful effects, and in the process release free radicals that damage or break down collagen. In this study, researchers mimicked this process of triggering a neutrophil immune response using Tumor Necrosis Factor alpha (TNF-a). When astaxanthin levels (5-50uM) were added to TNF-a primed neutrophils, a dose-dependent restraint was found in free radical activity released by neutrophils, and subsequent collagen I and III damage. The addition of 50 uM of astaxanthin prevented collagen I loss by 28.1±8%, and collagen III loss by 49.4±5.9%. It also did not impact cell numbers, or affect the expression of collagen I and III, which shows astaxanthin's effect is specific to the inhibition of free radical activity released by neutrophils, which damage cells during a response to environmental triggers (e.g., exposure to sunlight). Not only does this indicate astaxanthin's potential for enhanced skin benefits, but since collagen makes up the majority of cartilage in knees and joints, it also indicates potential support for those with an active lifestyle. Lycored's astaxanthin uses algae-derived (Haematococcus pluvialis) astaxanthin carotenoid with anti-oxidative and anti-inflammatory properties that provides a multitude of science-backed benefits. Previous studies have also linked astaxanthin to positive sports recovery results, cellular health, heart health, joint health, immune systems support and mitigation of oxidative damage and inflammation. Astaxanthin cannot be synthesized by humans and, therefore, must be consumed in the diet via food or supplements. Elizabeth Tarshish, PhD, Head of Claims and Clinical Affairs at Lycored states, "This study not only showcases astaxanthin's capabilities for skin health, but also underscores how beneficial astaxanthin can be for joint health and recovery for active individuals. At Lycored, we strive to help people discover beauty from within, so we're excited about what these results mean for the preventive health benefits of astaxanthin throughout the body." View the full study at: Astaxanthin Supports Normal Human Dermal Fibroblasts from Neutrophil-Induced Collagen Damage in Co-Culture (starlingscience.com) About Lycored Lycored is an international company at the forefront of discovering beauty from within by combining nature's goodness with cutting edge science to deliver a sensory journey that impacts wellbeing. Established in 1995 in Israel, Lycored is the global leader in natural carotenoids for food, beverage and dietary supplement products. For more information visit . Contact Katie Hill Padilla +1 480 285 8148 [email protected] Logo - SOURCE Lycored

临床结果

2023-03-24

·NS Medical Devices

Cambrium Launches NovaColl: First Micro-Molecular & Skin-Identical Collagen Ingredient for Personal Care Industry

The biotechnology company will debut its first product at the in-cosmetics Global trade show from March 28-30, in Barcelona NovaColl product image. (Credit: GlobeNewswire, Inc./ Cambrium) Biotechnology company Cambrium has launched its first product: NovaColl. It is the first micro-molecular & 100% skin-identical collagen specifically designed for premium personal care formulations. Owing to its structure and small molecular size, NovaColl is able to create powerful collagen benefits across skin layers. This new class of collagen active is vegan certified and produced through Cambrium’s precision fermentation process, offering formulators a unique combination of efficacy, skin-compatibility and sustainability. NovaColl’s claims have been scientifically validated in third party in vitro studies, which demonstrated a broad range of activities working to holistically protect, maintain and enhance native collagen. Beyond bio-similarity, NovaColl is 100% skin-identical, offering previously inaccessible levels of biocompatibility for personal care applications. To achieve this, Cambrium’s multi-disciplinary team of scientists and engineers searched through billions of protein sequences for skin-active features and indicators of efficacy. They identified NovaColl, a highly bioactive region of collagen I, the most prevalent collagen type in our human skin. Rather than extracting collagen from animals, Cambrium uses yeast cells to grow their micro-molecular collagen in a cruelty-free and sustainable fermentation process, minimizing energy, land, and water use. Source: Company Press Release