更新于：2024-11-01

KLK4

更新于：2024-11-01

基本信息

别名

EMSP、EMSP1、enamel matrix serine proteinase 1

+ [10]

简介

Has a major role in enamel formation (PubMed:15235027). Required during the maturation stage of tooth development for clearance of enamel proteins and normal structural patterning of the crystalline matrix (By similarity).

关联

项与 KLK4 相关的药物

WO2024052922

专利挖掘

靶点

KLK4

作用机制-

在研机构

Yeda Research & Development Co. Ltd.

原研机构

Yeda Research & Development Co. Ltd.

在研适应症

肿瘤

非在研适应症-

最高研发阶段药物发现

首次获批国家/地区-

首次获批日期1800-01-20

100 项与 KLK4 相关的临床结果

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100 项与 KLK4 相关的转化医学

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0 项与 KLK4 相关的专利（医药）

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761

项与 KLK4 相关的文献（医药）

2024-12-01·Mammalian Genome

Comprehensive analysis of chromosomal breakpoints and candidate genes associated with male infertility: insights from cytogenetic studies and expression analyses

Article

作者: Kakavand, Kianoush ; Sabbaghian, Marjan ; Hassanzadeh, Vahideh ; Masoudi, Najmeh Sadat ; Meybodi, Anahita Mohseni ; Moradi, Shabnam Zarei ; Shahhoseini, Maryam ; Zamanian, Mohammadreza ; Hossein Garakani, Melika ; Ghaheri, Azadeh

The study aimed to investigate prevalent chromosomal breakpoints identified in balanced structural chromosomal anomalies and to pinpoint potential candidate genes linked with male infertility. This was acchieved through a comprehensive approach combining RNA-seq and microarray data analysis, enabling precise identification of candidate genes. The Cytogenetics data from 2,500 infertile males referred to Royan Research Institute between 2009 and 2022 were analyzed, with 391 cases meeting the inclusion criteria of balanced chromosomal rearrangement. Of these, 193 cases exhibited normal variations and were excluded from the analysis. By examining the breakpoints, potential candidate genes were suggested. Among the remaining 198 cases, reciprocal translocations were the most frequent anomaly (129 cases), followed by Robertsonian translocations (43 cases), inversions (34 cases), and insertions (3 cases).Some patients had more than one chromosomal abnormality. Chromosomal anomalies were most frequently observed in chromosomes 13 (21.1%), 14 (20.1%), and 1 (16.3%) with 13q12, 14q12, and 1p36.3 being the most prevalent breakpoints, respectively. Chromosome 1 contributed the most to reciprocal translocations (20.2%) and inversions (17.6%), while chromosome 14 was the most involved in the Robertsonian translocations (82.2%). The findings suggested that breakpoints at 1p36.3 and 14q12 might be associated with pregestational infertility, whereas breakpoints at 13q12 could be linked to both gestational and pregestational infertility. Several candidate genes located on common breakpoints were proposed as potentially involved in male infertility. Bioinformatics analyses utilizing three databases were conducted to examine the expression patterns of 78 candidate genes implicated in various causes of infertility.‏ In azoospermic individuals, significant differential expression was observed in 19 genes: 15 were downregulated (TSSK2, SPINK2, TSSK4, CDY1, CFAP70, BPY2, BTG4, FKBP6, PPP2R1B, SPECC1L, CENPJ, ‏SKA3, FGF9, NODAL, CLOCK), while four genes were upregulated ‏(‏HSPB1, MIF, PRF1, ENTPD6). In the case of Asthenozoospermia, seven genes showed significant upregulation (PRF1, DDX21, KIT, SRD5A3, MTCH1, DDX50, NODAL). Though RNA-seq data for Teratozoospermia were unavailable, microarray data revealed differential expression insix genes: three downregulated (BUB1, KLK4, PIWIL2) and three upregulated (AURKC, NPM2, RANBP2). These findings enhance our understanding of the molecular basis of male infertility and could provide valuable insights for future diagnostic and therapeutic strategies.

2024-12-01·Science of The Total Environment

The succession of microbial community and distribution resistance gene in response to enrichment cultivation derived from a long-term toxic metal(loid)s polluted soil

Article

作者: Shi, Jiaxin ; Liu, Hongwei ; Yang, Jiejie ; Liang, Yili ; Liu, Xueduan ; Jiang, Luhua ; Peng, Yulong ; Miao, Bo ; Tang, Wangwang ; Al-Dhabi, Naif Abdullah ; Yin, Huaqun ; Guo, Ziwen

Microbial communities as the most important and active component of soil play a crucial role in the geochemical cycling of toxic metal(loid)s in the Pb and Zn smelting site soils. However, the relationships between soil microbial communities and the fractions of toxic metal(loid)s and the succession of soil microbial community and functions after enrichment cultivation have rarely been analyzed. In this study, the diversity and composition of microbial communities in soils before and after enrichment cultivation were investigated by high-throughput sequencing. And the co-occurrence relationships between soil microbial community after enrichment cultivation and MRGs genes were also analyzed through the BacMet database. Results showed that the dominant genus in the soils was Lactobacillus and Stenotrophomonas. The soil microbial community exhibited a notable correlation with Cd, Pb, and As, among which Cd exerted the most profound impact. Alishewanella, Pseudomonas, Massilia and Roseibacillus were significantly correlated with the fraction of Cd. After enrichment cultivation, the number of genera decrease to 96. And the dominant genus changed to Acinetobacter, Bacillus, Comamonas, Lysobacter, and Pseudoxanthomonas. High abundance of metal resistance genes (MRGs) including zntA, fpvA, zipB, cadA, czcA, czcB, czcC, zntA, arsR, pstS and pstB was found in the microbial community after enrichment cultivation. The potential host genus for MRGs was Acinetobacter, Comamonas, Lysinibacillus, Azotobacter, Bacillus, Lysobacter, Cupriavidus, Pseudoxanthomonas, and Thermomonas. Additionally, these microbial community after enrichment cultivation possessing pathways of bacterial chemotaxis and two-component systems was enabled them to adapt to the polluted environment. These observations provided potential guidance for microbe isolation and the development of strategies for the bioremediation of toxic metal(loid)s polluted soils.

2024-12-01·Current Opinion in Biotechnology

Phosphate-binding proteins and peptides: from molecular mechanisms to potential applications

Review

作者: McLamore, Eric S ; Mayer, Brooke K ; Venkiteshwaran, Kaushik ; Hutchison, Justin M ; Torres, Maria

Selective binding of phosphate is vital to multiple aims including phosphate transport into cells and phosphate-targeted applications such as adsorption-based water treatment and sensing. High-affinity phosphate-binding proteins and peptides offer a nature-inspired means of efficiently binding and separating phosphate from complex matrices. The binding protein PstS is characterized by a Venus flytrap topology that confers exceptional phosphate affinity and selectivity, and is effective even at low phosphate concentrations, all of which are essential for applications such as phosphate sensing, removal, and recovery. The binding event is reversible under controlled conditions, making it germane to catch-and-release objectives that advance phosphorus sustainability. Peptides such as the P loop motif are also promising for such applications. Future advances in protein/peptide design can contribute to increased implementation in engineered systems.