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更新于：2025-05-07

RNase H

更新于：2025-05-07

基本信息

别名

endoribonuclease H、RNase H、RNH

简介-

关联

项与 RNase H 相关的药物

CA3218205

专利挖掘

靶点

RNase H

作用机制-

在研机构

Q-State Biosciences, Inc.

原研机构

Q-State Biosciences, Inc.

在研适应症

神经系统表现

非在研适应症-

最高研发阶段药物发现

首次获批国家/地区-

首次获批日期1800-01-20

100 项与 RNase H 相关的临床结果

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100 项与 RNase H 相关的转化医学

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0 项与 RNase H 相关的专利（医药）

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3,306

项与 RNase H 相关的文献（医药）

2025-10-01·Talanta

A triple-helix molecular switch-based fluorescent aptasensor using Klenow fragment-assisted target recycling and Ribonuclease H-powered DNA walker cascade amplification for detection of MRSA

Article

作者: Xu, Jiaxin ; Lu, Fangguo ; Zhang, Zidong ; Ning, Yi

MRSA is an antibiotic resistant bacterium that poses a significant threat to the environment and human health due to its bioaccumulation and potential widespread contamination. The prompt and accurate identification of MRSA is essential for enhancing environmental monitoring and clinical management. Here, we develop a triple-helix molecular switch (THMS) fluorescent aptasensor for the determination of MRSA using Klenow fragment (KF)-assisted target recycling and Ribonuclease H (Rnase H)-powered DNA walker cascade amplification. In this method, the target opens the THMS by specifically binding with the aptamer, resulting in the release of target/aptamer complex and DNA walker. KF then initiates the target recycling process via strand-displacement polymerization reaction under the assistance of carboxyfluorescein (FAM)-labeled primer and dNTPs, creating plenty of double-stranded DNA (dsDNA) products. These dsDNA products show low affinity to graphene oxide (GO) and generate strong fluorescence. This fluorescence is considerably significantly amplified in the presence of SYBR Green I (SGI), attributable to the synergistic interaction between dsDNA and SGI. In the interim, Rnase H drives the released DNA walker to automatically walk on the carboxylated graphene oxide surface by cleaving FAM-labeled RNA signal probe (SP), causing the FAMs to dissociate from the carboxylated graphene oxide (CGO). Therefore, fluorescent signal originating from the two reaction pathways can be detected at excitation/emission wavelengths of 480/514 nm. The target measured by this strategy demonstrates a broad linear working range from 10² colony-forming units (CFU)/mL to 10⁷ CFU/mL, with a detection limit (LOD) of 15 CFU/mL. Moreover, this method performs well in milk and pus sample analysis. These results reveal that this aptasensor is highly specific and sensitive for detecting MRSA and is endowed with good potential for food monitoring and clinical diagnosis applications.

2025-05-31·Clinical Psychopharmacology and Neuroscience

Association of Human Endogenous Retrovirus HERV-K18 Variant with Bipolar Disorder Type I

Article

作者: Sarisoy, Gokhan ; Aral, Ayse Erguner ; Yegin, Zeynep ; Avsar, Cumhur ; Koc, Haydar

ObjectiveHuman endogenous retroviruses (HERVs) and associated sequences occupy ∼8% of the human genome and dysregulation of HERV transcripts may have significant impacts on human health including psychiatric disorders. HERV-K18 is still active in the human genome and its envelope gene encodes a superantigen (SAg) which may result in deregulation of the immune system. In the study, the possible associations of the two variants localized in the SAg-coding region of HERV-K18 with bipolar disorder type I (BD-I) were evaluated.MethodsThe subjects included 100 patients with BD-I and 100 age- and sex-matched healthy controls. The effects of the two HERV-K18 variants (HERV-8594 and HERV-8914) were analyzed with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The possible associations of the genotypes/alleles in BD-I patients with several clinical and demographic data were also evaluated.ResultsHERV-8914 TT genotype had approximately 5.36 times higher risk of BD-I than those with the CC genotype (odds ratio, 5.386; 95% confidence interval, 1.602-18.110). Moreover, the prevalence of the CC genotype in patients with hypomania (31.25%) was found to be higher than that observed in patients without hypomania (10.71%) (Fisher's exact test = 5.931, p = 0.036).ConclusionThis is the first study implying that HERV-K18 variations may be associated with the pathogenesis of BD-I.

2025-04-10·Nucleic Acids Research

UV induces codirectional replication–transcription conflicts and an alternative DnaA-dependent replication origin in the rnhA B mutants of Escherichia coli

Article

作者: Cronan, Glen E ; Kuzminov, Andrei ; Kouzminova, Elena A

AbstractThe rnhAB mutants of Escherichia coli lacking both RNase H enzymes are unexpectedly UV-sensitive, being unable to restore normal levels of post-UV replication. Examining patterns of chromosomal replication in the rnhAB mutants after UV could identify the problem sites. We show that normal rnhA (B) mutant replication initiates at three distinct oriK areas in the origin macrodomain, none of them coinciding with oriC proper, the dominant origin being some 400 kb away. Interestingly, initiation after UV switches to the DnaA-dependent oriK closest to oriC and continues from there until the growth replication pattern is restored, like in the rnhA single mutants. However, in the rnhAB double mutant, post-UV forks initiated at the new origin have difficulty reaching the terminus, with the major stalling sites at the rrn operons. In the rnhAB recBC mutants, additionally deficient in linear DNA degradation/repair, post-UV replication forks cannot traverse the origin-distal ribosomal RNA operons, rrnG and rrnH, showing that restoration of disintegrated replication forks is essential for replication in the rnhAB mutant. In contrast, the rnhAB rpoB* mutant, in which transcription complexes are unstable, is UV-resistant and resumes normal replication even faster than WT cells, indicating that the rnhAB mutants suffer from UV-induced replication–transcription conflicts.

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