The purpose of this research study is to determine whether the amount of radiation given to the normal areas around the anal cancer can be reduced by using Proton Therapy while reducing the side effects that are seen with standard therapy.

开始日期2017-01-04

申办/合作机构

University of Cincinnati (Ohio)

EUCTR2010-020348-36-GB / Not yet recruitingN/A

Randomized double-blind Phase III trial of FOLF(HA)iri vs FOLFIRI forsecond or third line therapy in irinotecan-naïve patients with metastaticcolorectal cancer - HA-Irinotecan Phase III trial ACO-002

开始日期2011-01-31

申办/合作机构-

NCT01151761 / Terminated临床2期IIT

Phase II Study of Stereotactic Body Radiotherapy (SBRT) and Chemotherapy for Unresectable Cholangiocarcinoma Followed by Liver Transplantation

The purpose of this study is to determine progression-free survival at 12 months for stereotactic body radiotherapy (SBRT) and chemotherapy for unresectable hilar cholangiocarcinoma (CCA).

开始日期2011-01-01

申办/合作机构

Stanford University

100 项与 TYMS x CD44 相关的临床结果

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100 项与 TYMS x CD44 相关的转化医学

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0 项与 TYMS x CD44 相关的专利（医药）

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项与 TYMS x CD44 相关的文献（医药）

2023-02-01·Neuropathology

Identification and characterization of sex‐dependent gene expression profile in glioblastoma

Article

作者: Chen, Kefu ; Qin, Shangyao ; Wu, Yulong ; Pu, Yingyan ; Liu, Hong ; Su, Zhida ; Yuan, Yimin

Glioblastoma (GBM) is the most lethal primary tumor in the human brain and lacks favorable treatment options. Sex differences in the outcome of GBM are broadly acknowledged, but the underlying molecular mechanisms remain largely unknown. To identify the sex‐dependent critical genes in the progression of GBM, raw data from several microarray datasets with the same array platform were downloaded from the Gene Expression Omnibus (GEO) database. These datasets included tumorous and normal tissue from patients with GBM and crucial sex features. Then, the differentially expressed genes (DEGs) in female and male tumors were identified via bioinformatics analysis, respectively. Functional signatures of the identified DEGs were further annotated by Gene Ontology (GO) and pathway enrichment analyses. Venn diagram and functional protein–protein interaction (PPI) network analyses were performed to screen out the sex‐specific DEGs. Survival analysis of patients with differences in the expression level of selected genes was then carried out using the data from The Cancer Genome Atlas (TCGA). Here, we showed that ECT2, AURKA, TYMS, CDK1, NCAPH, CENPU, OIP5, KIF14, ASPM, FBXO5, SGOL2, CASC5, SHCBP1, FN1, LOX, IGFBP3, CSPG4, and CD44 were enriched in female tumor samples, whereas TNFSF13B, CXCL10, CXCL8, CXCR4, TLR2, CCL2, and FCGR2A were enriched in male tumor samples. Among these key genes, interestingly, ECT2 was associated with increased an survival rate for female patients, whileTNFSF13B could be regarded as a potential marker of poor prognosis in male patients. These results suggested that sex differences in patients may be attributed to the heterogeneous gene activity, which might influence the oncogenesis and the outcomes of GBM.

2022-09-01·Biomedicine & Pharmacotherapy

Disulfiram increases the efficacy of 5-fluorouracil in organotypic cultures of colorectal carcinoma

Article

作者: Vlček, Petr ; Vašíčková, Jana ; Šmarda, Jan ; Hermanová, Markéta ; Beneš, Petr ; Říhová, Kamila ; Stiborek, Marek ; Hendrych, Michal ; Navrátilová, Jarmila ; Preisler, Jan ; Adamová, Barbora ; Kanický, Viktor ; Hradil, Vojtěch

Drug efficacy determined in preclinical research is difficult to transfer to clinical practice. This is mainly due to the use of oversimplified models omitting the effect of the tumor microenvironment and the presence of various cell types participating in the formation of tumors in vivo. In this study, we used robust three-dimensional models including spheroids grown from colon cancer cell lines and organotypic cultures prepared from the colorectal carcinoma tissue to test novel therapeutic strategies. We developed a multi-modal approach combining brightfield and fluorescence microscopy for evaluating drug effects on organotypic cultures. Combined treatment with 5-fluorouracil and disulfiram/copper efficiently eliminated cancer cells in these 3D models. Moreover, disulfiram/copper down-regulated the expression of markers associated with 5-fluorouracil resistance, such as thymidylate synthase and CD133/CD44. Thus, we propose combined therapy of 5-fluorouracil and disulfiram/copper for further testing as a treatment for colorectal carcinoma. In addition, we show that organotypic cultures are suitable models for anti-cancer drug testing.

2022-01-01·American journal of cancer research3区 · 医学

TYMS-TM4SF4 axis promotes the progression of colorectal cancer by EMT and upregulating stem cell marker.

3区 · 医学

Article

作者: Ye, Jiecheng ; Guo, Wenjing ; Zhang, Fen ; Zhang, Fenfen ; Han, Anjia ; Wang, Liyuan

BACKGROUNDThe expression of thymidylate synthase (TYMS) is significantly up-regulated in various cancers and associated with the poor prognosis of patients. However, the role of TYMS in the progression of colorectal cancer (CRC) is unclear.METHODSCell function assay, biology information analysis, and RNA sequencing were used to investigate the role of TYMS in the progression of CRC and underlining molecular mechanism. SPSS22.0 statistical software and GraphPad Prism 5 (Graphpad software) were used for statistical analysis.RESULTSOur results showed that TYMS expression was higher in CRC tissues than that in non-tumor colorectal mucosa tissues. TYMS knockdown inhibited the proliferation, migration and invasion of HCT116 and HT29 cells, and the spheroid formation of HCT116 cells. The underling mechanism demonstrated that TYMS promoted the progression of CRC by regulating EMT-related proteins including E-cadherin, Vimentin, MMP-9 and stem cell biomarkers including CD133 and CD44. Furthermore, DEG sequencing showed that TYMS knockdown enriched the pathways of metastasis and metabolism by GO and KEGG analysis. We identified TM4SF4 was the downstream target of TYMS in CRC cells. TM4SF4 overexpression increased migration and invasion of CRC cells by regulating EMT and CD133 expression.CONCLUSIONSOur findings suggest that TYMS-TM4SF4 axis may promote the progression of CRC by EMT and upregulating stem cell markers.